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Upload si/shRNA file
si/shRNA File
:
Select the file containing the si/shRNAs.
Indicate File Type
:
Input file contains both active and inactive RNAi reagents
Input file contains only active RNAi reagents
Sample File
Indicate input strand
:
Input file contain sense (passenger) strand sequences
Input file contain antisense (guide) strand sequences
Indicate RNAi reagent type
:
Input file contains siRNA sequences
Input file contains shRNA sequences
Do you want to trim shRNA sequences
:
No trim is needed
Trim one nucleotide
Trim two nucleotides
Trim three nucleotides
Indicate reference data types or upload database file
Database
:
Use built in database
Upload custom database file
Region
:
3'UTR
5'UTR
CDS
Full transcript for protein coding genes
Full transcript for all genes
Organism
:
Human
Mouse
Drosophila
Custom Database File:
Select the file containing sequences
Options
Seed sequence length
:
6
7
8
Minimum number of seed matches
:
1
2
3
4
Choose strand(s) to use
:
Antisense (Guide) only
Sense (Passenger) only
Both
Either
p-value
:
+ Advanced Options
Which transcripts do you want to be listed in the results file:
Full list
Only Significant transcripts (p value <= 0.05)
Would you like to include transcripts that are enriched in the background to the results file?
No
Yes
Would you like to scramble all si/shRNA seed sequences as control to run a parallel test?
No
Yes
Enter a name for your job (Required):
Submit