RNA interference (RNAi) is widely used for loss-of-function
studies.The biological relevance of these studies depends on the
appropriate mapping of reagents to genes. At first glance, matching
RNAi reagents to genes appears to be a simple problem. One reagent is
typically designed to target only one gene, but the reagent-gene
relationship is complex. The sequences of things like the
oligonucleotides used to generate RNAi reagents are static but the
reference genome sequences and gene annotations are regularly updated.
Thus, when a researcher chooses an RNAi reagent or analyzes data, the
most current interpretation of the target and specificity of the RNAI
reagent might have changed. UP-TORR provides accurate and up-to-date
annotation of cell-based and in vivo RNAi reagents targeting Drosophila
genes, including reagents from all major public collections (DRSC,
DKFZ, NIG-Japan, VDRC and TRiP). UP-TORR can also provide up-to-date
annotation for RNAi reagents targeting C. elegans, mouse or
human genes (you will need to enter the reagent sequences).
For citations, please use:
Hu Y, Roesel C, Flockhart I, Perkins L,
Perrimon N, Mohr S. UP-TORR: Online Tool for Accurate and
Up-to-Date Annotation of RNAi
Reagents. Genetics. 2013 Sep;195(1):37-45. doi:
10.1534/genetics.113.151340. Epub 2013 Jun 21.