TF2TG User Guide

Introduction

TF2TG (“transcription factor” to “target gene") was developed as a comprehensive resource that combines both in vitro and in vivo datasets to link transcription factors (TFs) to their target genes based on TF–DNA binding preferences along with the protein-protein interaction data, tissue-specific transcriptomic data, and chromatin accessibility data. TF2TG allows researchers to inquire: 1.) What TFs might regulate a given gene by binding to the promoter, intron or downstream of a gene? 2.) What are the potential target genes (TG) of a given TF? 3.) What are the potential TF binding sites in this chromosome region of interesting? 4.) What are the potential TF binding sites in the sequence of input? 5.) What distance enhance region interact with the chromosome region of interest by Hi-C data and all the potential binding sites found in this region as well as the interacting regions?

Instructions

1.) What TFs might regulate a given gene by binding to the promoter, intron or downstream of a gene?

Figure 1

2.) What are the potential target genes (TG) of a given TF?

Figure 2

3.) What are the potential TF binding sites in this chromosome region of interesting?

4.) What are the potential TF binding sites in the input sequence?

Figure 3

5.) What distance enhance regions interact with the chromosome region of interest by Hi-C data and all the potential TF binding sites found in this region as well as the interacting regions?

Figure 4