PathON - Pathway Analysis Tool

PathON - Pathway Analysis Tool For Drosophila

1. Enter Data (choose data format first)

a. Select Type of Input
b. Select Type of Input
Enter genes or proteins, score (one per line)

Upload from file (txt, csv or xlsx). more info about input files (new page)

2. Choose Analysis:

Pathway specific transcription factor target genes

Pathway core components

Confidence:

high

high and moderate

3. Choose Threshold:

Threshold by Value (values above and under the - of this threshold)
Threshold by Percent (values in the top and bottom %)

Example of input files:

Tab/comma delimited text file or excel spread sheet of RNA-Seq data (eg. FPKM or TPM values) for control and experiment samples. Please name the column headers according to the example file

FBgn	control1	control2	data1	data2
FBgn0000003	259508	294806	296957	322142
FBgn0000008	1.056016	0.871348	7.8228371	7.371974
FBgn0000014	3.426636	1.892972	2.948463	3.357747
FBgn0000015	1.706773	0.796946	0.6492518	0.5241473
FBgn0000017	2.125105	1.005055	3.083105	0.5969986
FBgn0000018	1.3666	1.15488	1.40831	0.985265
FBgn0000022	0	0.060552	0.298234	0.0127875

Tab/comma delimited text file or excel spread sheet of RNA-Seq data (eg. FPKM or TPM values) for one type of sample with or without replicates

FBgn	data1	data2
FBgn0000042	199.3337	405.0041
FBgn0000043	19.5393	60.9437
FBgn0000044	34.2968	13.0075
FBgn0000045	0.511129	0.423392
FBgn0000046	3.324057	3.495537
FBgn0000047	0.473196	0.28821
FBgn0000052	29.25787	35.66972

Tab/comma delimited text file or excel spread sheet of pre-calculated ratios (eg. log2 ratio of the fold changes of experimental vs control)

FBgn	Ratio
FBgn0000008	2.88565534
FBgn0000014	0.24545322
FBgn0000015	-0.4365741
FBgn0000017	0.38343027
FBgn0000018	-0.0662497
FBgn0000022	0