FlyPredictome: A structural atlas of predicted protein–protein interactions in Drosophila

FlyPredictome is an open resource for structurally resolved Drosophila protein–protein interactions (PPIs). We used AlphaFold-Multimer to predict pairwise complexes at proteome scale, scored each with the integrated Local Interaction Score (iLIS) — a local-confidence metric that performs robustly for flexible and intrinsically disordered proteins — and made every prediction searchable at residue-level resolution.

Browse the atlas, search any Drosophila protein pair, retrieve structural models with PAE heatmaps and per-residue interface annotations, or explore the interactome network interactively.

• iLIS — local confidence metric that captures PPI involving flexible and disordered proteins (10% FPR threshold: iLIS ≥ 0.223). Code: https://github.com/flyark/AFM-LIS
• Evidence-supported network viewer — hierarchically clustered PPI network organized into functional modules. https://flyark.github.io/FlyPredictome-network
• Kim, A.-R., Comjean, A., Veal, A., Rodiger, J., Han, M., Hu, Y., Perrimon, N. (2026). FlyPredictome: A structural atlas of predicted protein–protein interactions in Drosophila. bioRxiv.