CRISPR GuideXpress

» Search for Orthologs
To search for orthologous genes between Drosophila melanogaster and Anopheles gambiae
(Gene set: AgamP4.12) enter an FBgn, Gene Symbol, or VectorBase ID, select methods, then
click the 'submit' button.

» Search for Mosquito CRISPR sgRNA Designs
To search for CRISPR sgRNA designs for gene knockout in Anopheles, Aedes, or Culex, enter a VectorBase ID or genomic location, then click the 'submit' button. Results shown on a summary page with a table and embedded JBrowse view.

CRISPR design in Drosophila melanogaster: https://www.flyrnai.org/crispr3

**NEW** features:
  • Search for orthologous genes between Drosophila melanogaster and supported mosquito species
  • Search for mosquito CRISPR sgRNA designs
  • Batch search by sgRNA sequences
  • Machine Learning based efficiency prediction
  • sgRNA Distance to Start Codon + % Coverage
  • sgRNA isoform coverage

Ortholog Search


Enter one ID per line.


Enter one ID per line.


Enter one ID per line.



sgRNA Search


Search by gene.

Search by genomic location.


sgRNA Batch Search



Enter one VectorBase ID or Gene Name per line.


Enter one gRNA sequence per line.