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ails Page displays data (positive and negative) submitted to the RSVP. It is up to you to interpret that data. To help you do this, it is important to keep a few points in mind:
- The RSVP captures both positive and negative data. In the example below, with two different GAL4 drivers, no phenotypes were obtained. This DOES NOT mean that the TRiP stock, HMS00833, does not work. It simply means that the sima gene may not function in the tissues where it was expressed.
There are cases where a TRiP Stock has been determined to be ineffective and has been removed from the collection. This will be noted on the RSVP Details Page for that Stock.
- There are many cases in the RSVP database where a particular TRiP line produces an expected and potent phenotype with one driver and no phenotype with another driver. This probably reflects that the gene is only required in some tissues.
- When using RSVP data to select a TRiP stock to use in your experiments make sure that you do not restrict your search only to TRiP lines with RSVP Validation Data. E.g., searching for FAK TRiP lines with RSVP validation data yields nothing. But searching for FAK TRiP lines regardless of RSVP data (on the Search Page: do not check the box at the lower right), yields 4 FAK TRiP stocks with no associated RSVP data.
- Below is the Search for msk (moleskin) in the RSVP database.
When choosing one or more TRiP stocks to work with, be sure to pick the stock that will work express at the appropriate stage in development. For example, if you are screening TRiP stocks to ID genes that affect embryonic muscle development, do not choose TRiP.GL00435, which works in the germline, but not embryonic muscles (below). For a complete description of the TRiP vectors and when and where in development they are designed to work, go to the TRiP website: Design Approach: TRiPSoma and TRiPGermline.
Three of these moleskin TRiP stocks work well in the soma (HMS00020, HMS01408, JF02727). Examining the Details Pages for each of these lines reveals that HMS00020 and JF02727 produce potent knockdown phenotypes with the Dmef2-R-Gal4 driver, while no phenotype is observed when HMS01408 is driven by the same GAL4 line. This is good evidence that HMS01408 may be an ineffective TRiP line, and HMS00020 and JF02727 are effective RNAi reagents.