Protocols

Protocol Search


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Automation

    publications

  • 2015 - Dexterous robotic manipulation of alert adult Drosophila for high-content experimentation. Savall J,Ho ET,Huang C,Maxey JR,Schnitzer MJ pmid: 26005812

Cell culture

    publications

  • 2008 - Flow cytometric analysis of Drosophila cells. de la Cruz AF,Edgar BA pmid: 18641961
  • 2016 - Analysis of Single-Locus Replication Timing in Asynchronous Cycling Cells. Federica LS pmid: 27659974
  • 2014 - Cell lines. Cherbas L,Gong L pmid: 24434506
  • 2008 - Drosophila cell lines as model systems and as an experimental tool. Baum B,Cherbas L pmid: 18641962
  • 2016 - Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression. Backovic M,Krey T pmid: 26820867
  • 2016 - Methods to Establish Drosophila Cell Lines. Debec A,Megraw TL,Guichet A pmid: 27730593
  • 2009 - Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening. Bai J,Sepp KJ,Perrimon N pmid: 19798083
  • 2007 - Drosophila cell culture and transformation. Cherbas L,Cherbas P pmid: 21357155
  • 2014 - Notch signaling assays in Drosophila cultured cell lines. Li J,Housden BE,Bray SJ pmid: 25053486
  • 2013 - Organelle transport in cultured Drosophila cells: S2 cell line and primary neurons. Lu W,Del Castillo U,Gelfand VI pmid: 24300413
  • 2015 - Insect cell entrapment, growth and recovering using a single-use fixed-bed bioreactor. Scaling up and recombinant protein production. Ventini-Monteiro D,Dubois S,Astray RM,Castillo J,Pereira CA pmid: 26481831

web_pages

  • Media preparation         ( https://dgrc.bio.indiana.edu/Protocols?tab=vectors )
  • Freezing and thawing         ( https://dgrc.bio.indiana.edu/Protocols?tab=vectors )
  • Fixed cell imaging         ( http://www.flyrnai.org/DRSC-Protocol-cellfix.html )
  • Fixed cell imaging         ( https://dgrc.bio.indiana.edu/Protocols?tab=vectors )
  • Media preparation         ( http://www.flyrnai.org/DRSC-PRC.html )
  • Transfection of cells         ( http://www.bio-protocol.org/e190 )
  • Embryonic cell line isolation         ( https://dgrc.bio.indiana.edu/Protocols?tab=vectors )
  • Stable transfected cell line production         ( http://www.flyrnai.org/DRSC-PRL.html )
  • 2009 - Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening.         ( http://www.flyrnai.org/DRSC-PCP.html )
  • Total ATP detection         ( http://www.flyrnai.org/DRSC-Protocol-atp-levels.html )
  • Single-cell cloning         ( http://www.flyrnai.org/DRSC-Protocol_single_cell_cloning.html )
  • Cell-based RNAi

      publications

    • 2014 - RNAi screening in Drosophila cells and in vivo. Mohr SE pmid: 24576618
    • 2016 - Methods for High-Throughput RNAi Screening in Drosophila Cells. Billmann M,Boutros M pmid: 27730577
    • 2008 - RNAi screening in cultured Drosophila cells. Steinbrink S,Boutros M pmid: 18641945
    • 2008 - High-throughput RNAi screen in Drosophila. DasGupta R,Gonsalves FC pmid: 19109710

    web_pages

  • Protocol notes-tagged entries at FlyRNAi blog         ( http://flyrnai.blogspot.com/search/label/protocol%20notes )
  • Arrayed RNAi screening         ( http://www.flyrnai.org/DRSC-PRR.html )
  • dsRNA synthesis         ( http://www.flyrnai.org/DRSC-PRS.html )
  • Clonal and mosaic analysis

      publications

    • 2012 - Power tools for gene expression and clonal analysis in Drosophila. del Valle Rodriguez A,Didiano D,Desplan C pmid: 22205518
    • 2014 - Generating mosaics for lineage analysis in flies. Lee T pmid: 24902835

    web_pages

  • FLP-FRT         ( http://www.bio-protocol.org/e1458 )
  • Conditional expression systems

      publications

    • 2016 - The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression. Caygill EE,Brand AH pmid: 27730574
    • 2016 - The Q-System: A Versatile Expression System for Drosophila. Riabinina O,Potter CJ pmid: 27730575
    • 2008 - The GAL4 system : a versatile system for the expression of genes. Elliott DA,Brand AH pmid: 18641942
    • 2015 - Improved and expanded Q-system reagents for genetic manipulations. Riabinina O,Luginbuhl D,Marr E,Liu S,Wu MN,Luo L,Potter CJ pmid: 25581800

    Cryopreservation

      publications

    • 2016 - Arginine and proline applied as food additives stimulate high freeze tolerance in larvae of Drosophila melanogaster. Kostal V,Korbelova J,Poupardin R,Moos M,Simek P pmid: 27489218

    Fluorescence in situ hybridization (FISH)

      publications

    • 2008 - Fluorescent in situ hybridization protocols in Drosophila embryos and tissues. Lecuyer E,Parthasarathy N,Krause HM pmid: 18641955
    • 2008 - High-resolution fluorescent in situ hybridization of Drosophila embryos and tissues. Lecuyer E,Necakov AS,Caceres L,Krause HM pmid: 21356853
    • 2015 - Simple method for fluorescence DNA in situ hybridization to squashed chromosomes. Larracuente AM,Ferree PM pmid: 25591075

    Fly basics

    DIS techniques

  • A convenient method for supplying food to Drosophila. Perry, C., A. Boseman, and R. Pu
  • An efficient, practical, and reliable Drosophila trap. Freda, P.J., and J.M. Braverman
  • Male-specific GFP marker strain of Drosophila melanogaster. Hayashi, S.
  • The Citric-Arabic-glycerated gum: An alternative to Foure gum. Munoz-Hernandez, A., and P. Ramos-Morales
  • The Dover wild type strain and four derived isogenic lines. Possidente, B., C. Norton, and D. Possidente
  • Pigmentation scoring method for Drosophila. Rajpurohit, S., and A.J. Marlon
  • An instant fly medium and a convenient method to dispense it. Kliethermes, C.L.
  • publications

  • 2007 - Maintenance of a Drosophila laboratory: general procedures. Ashburner M,Roote J pmid: 21357030
  • 2016 - Maintenance of a Drosophila melanogaster Population Cage. Caravaca JM,Lei EP pmid: 27023790
  • 2008 - Getting started : an overview on raising and handling Drosophila. Stocker H,Gallant P pmid: 18641939
  • 2016 - Using FlyBase, a Database of Drosophila Genes and Genomes. Marygold SJ,Crosby MA,Goodman JL pmid: 27730573
  • 2007 - Drosophila embryo collection. Rothwell WF,Sullivan W pmid: 21357165
  • 2007 - Drosophila embryo dechorionation. Rothwell WF,Sullivan W pmid: 21357166
  • 2008 - Antibody staining of Drosophila tissues--helpful hints. Rebay I,Fehon RG pmid: 21356899
  • 2008 - Preparation of baskets for Drosophila egg collections, treatments, and incubations. Shermoen AW pmid: 21356900
  • videos

  • Fly Nomenclature         ( https://www.youtube.com/embed/vauo17YoF6w?list=PLBXEefaOGKebpBonK6m_Xl...... )
  • Observing Phenotypes and Crossing Drosophila melanogaster         ( https://www.youtube.com/embed/DkiCFkB9cSo )
  • Drosophila Melanogaster Mites         ( https://www.youtube.com/embed/PfSAAsrVANc?list=PLBXEefaOGKebpBonK6m_Xl...... )
  • Fly Basics 1: Girls and Boys         ( https://www.youtube.com/embed/64Cc_HLIvK8?list=PLBXEefaOGKebpBonK6m_Xl...... )
  • Anesthetizing Drosophila melanogaster         ( https://www.youtube.com/embed/S7FkmBjrnAs )
  • https://www.youtube.com/embed/DkiCFkB9cSo?list=PLBXEefaOGKebpBonK6m_Xlazv6nuMtvEh
  • Sexing Drosophila melanogaster: How to tell the sexes apart         ( https://www.youtube.com/embed/qfZlafhFjec )
  • Fruit Fly Developmental Stages         ( https://www.youtube.com/embed/DsK9R-yfgF4?list=PLBXEefaOGKebpBonK6m_Xl...... )
  • Drosophila Media Preparation         ( https://www.youtube.com/embed/Rph1xnB1_aw )
  • web_pages

  • Media preparation         ( http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/media-recipes....... )
  • Stock keeping         ( http://flystocks.bio.indiana.edu/Fly_Work/culturing.htm#stockkeeping )
  • Collection of virgin flies         ( http://flystocks.bio.indiana.edu/Fly_Work/culturing.htm#virgins )
  • Addressing mites         ( http://flystocks.bio.indiana.edu/Fly_Work/culturing.htm#mites )
  • Grape juice agar plates         ( http://theduroniolab.web.unc.edu/files/2013/10/Grape-Juice-Agar-Plates...... )
  • Genetic analysis         ( http://flystocks.bio.indiana.edu/Fly_Work/culturing.htm#matings )
  • Nomenclature         ( http://flystocks.bio.indiana.edu/Fly_Work/nomenclature.htm )
  • Addressing microbe infection         ( http://flystocks.bio.indiana.edu/Fly_Work/culturing.htm#microbes )
  • Genome engineering and mutagenesis

      books

    publications

  • 2014 - Targeted genome engineering techniques in Drosophila. Beumer KJ,Carroll D pmid: 24412316
  • 2014 - CRISPR/Cas9 mediated genome engineering in Drosophila. Bassett A,Liu JL pmid: 24576617
  • 2016 - Design and Generation of Donor Constructs for Genome Engineering in Drosophila. Housden BE,Perrimon N pmid: 27587780
  • 2016 - deGradFP: A System to Knockdown GFP-Tagged Proteins. Caussinus E,Affolter M pmid: 27730581
  • 2016 - Methods for TALEN-Mediated Genomic Manipulations in Drosophila. Liu J,Guo Y,Li C,Chen Y,Jiao R pmid: 26443222
  • 2016 - Creating Heritable Mutations in Drosophila with CRISPR-Cas9. Port F,Bullock SL pmid: 27730579
  • 2014 - Chemical mutagens, transposons, and transgenes to interrogate gene function in Drosophila melanogaster. Venken KJ,Bellen HJ pmid: 24583113
  • 2016 - Design and Generation of Drosophila Single Guide RNA Expression Constructs. Housden BE,Hu Y,Perrimon N pmid: 27587779
  • 2008 - The use of P-element transposons to generate transgenic flies. Bachmann A,Knust E pmid: 18641941
  • 2014 - Genetic screens to identify new Notch pathway mutants in Drosophila. Giagtzoglou N pmid: 25053478
  • 2015 - Genome engineering: Drosophila melanogaster and beyond. Venken KJ,Sarrion-Perdigones A,Vandeventer PJ,Abel NS,Christiansen AE,Hoffman KL pmid: 26447401
  • 2008 - EMS screens : from mutagenesis to screening and mapping. Bokel C pmid: 18641944
  • 2014 - Genome engineering with TALENs and ZFNs: repair pathways and donor design. Carroll D,Beumer KJ pmid: 24704173
  • 2014 - TALEN-mediated Drosophila genome editing: protocols and applications. Liu J,Chen Y,Jiao R pmid: 24751823
  • 2008 - P-element mutagenesis. Hummel T,Klambt C pmid: 18641943
  • 2013 - Using mutants, knockdowns, and transgenesis to investigate gene function in Drosophila. St Johnston D pmid: 24014449
  • 2014 - A simple TALEN-based protocol for efficient genome-editing in Drosophila. Zhang X,Ferreira IR,Schnorrer F pmid: 24680697
  • 2008 - Recombinases and their use in gene activation, gene inactivation, and transgenesis. Bischof J,Basler K pmid: 18641947
  • web_pages

  • Choosing a CRISPR-Cas9 strategy         ( http://www.crisprflydesign.org/choose-a-strategy/ )
  • gRNA cloning         ( http://www.crisprflydesign.org/grna-expression-vectors/ )
  • Mutation detection         ( http://www.crisprflydesign.org/t7-endo-i-assay/ )
  • gRNA design         ( http://www.crisprflydesign.org/avoid-off-targets/ )
  • CRISPR-tagged entries at FlyRNAi blog         ( http://flyrnai.blogspot.com/search/label/CRISPRs )
  • gRNA transcription         ( http://www.crisprflydesign.org/grnatranscription/ )
  • Mutation detection         ( http://www.crisprflydesign.org/direct-sequencing/ )
  • Imaging probes and approaches

    publications

  • 2015 - A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Yu D,Baird MA,Allen JR,Howe ES,Klassen MP,Reade A,Makhijani K,Song Y,Liu S,Murthy Z,Zhang SQ,Weiner OD,Kornberg TB,Jan YN,Davidson MW,Shu X pmid: 26098020
  • 2012 - Elastic volume reconstruction from series of ultra-thin microscopy sections. Saalfeld S,Fetter R,Cardona A,Tomancak P pmid: 22688414
  • 2015 - High-performance probes for light and electron microscopy. Viswanathan S,Williams ME,Bloss EB,Stasevich TJ,Speer CM,Nern A,Pfeiffer BD,Hooks BM,Li WP,English BP,Tian T,Henry GL,Macklin JJ,Patel R,Gerfen CR,Zhuang X,Wang Y,Rubin GM,Looger LL pmid: 25915120
  • 2012 - Faster STORM using compressed sensing. Zhu L,Zhang W,Elnatan D,Huang B pmid: 22522657
  • 2012 - Fiji: an open-source platform for biological-image analysis. Schindelin J,Arganda-Carreras I,Frise E,Kaynig V,Longair M,Pietzsch T,Preibisch S,Rueden C,Saalfeld S,Schmid B,Tinevez JY,White DJ,Hartenstein V,Eliceiri K,Tomancak P,Cardona A pmid: 22743772
  • 2015 - 3D high- and super-resolution imaging using single-objective SPIM. Galland R,Grenci G,Aravind A,Viasnoff V,Studer V,Sibarita JB pmid: 25961414
  • 2012 - Multiview light-sheet microscope for rapid in toto imaging. Krzic U,Gunther S,Saunders TE,Streichan SJ,Hufnagel L pmid: 22660739
  • 2012 - Multicolor two-photon tissue imaging by wavelength mixing. Mahou P,Zimmerley M,Loulier K,Matho KS,Labroille G,Morin X,Supatto W,Livet J,Debarre D,Beaurepaire E pmid: 22772730
  • 2012 - Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Cocheme HM,Logan A,Prime TA,Abakumova I,Quin C,McQuaker SJ,Patel JV,Fearnley IM,James AM,Porteous CM,Smith RA,Hartley RC,Partridge L,Murphy MP pmid: 22517261
  • web_pages

  • Propidium iodide staining         ( http://theduroniolab.web.unc.edu/files/2013/10/Propidium-Iodide-Staini...... )
  • Cobalt staining of Pupae         ( http://theduroniolab.web.unc.edu/files/2013/10/Cobalt-staining-of-pupa...... )
  • DAB staining         ( http://theduroniolab.web.unc.edu/files/2013/10/DAB-Staining.pdf )
  • In situ hybridization

    In vivo RNAi

      publications

    • 2016 - Design and Methods of Large-Scale RNA Interference Screens in Drosophila. Zhou J,Tong C pmid: 27581292
    • 2016 - A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila. Kaya-Copur A,Schnorrer F pmid: 27730578
    • 2014 - A Drosophila RNAi collection is subject to dominant phenotypic effects. Green EW,Fedele G,Giorgini F,Kyriacou CP pmid: 24577271

    web_pages

  • in vivo RNAi-tagged entries at FlyRNAi blog         ( http://flyrnai.blogspot.com/search/label/in%20vivo%20RNAi )
  • dsRNA injection into Larvae         ( https://www.scientificprotocols.org/protocols/injection-of-dsrna-into-...... )
  • Live imaging

      publications

    • 2014 - Live imaging in Drosophila: The optical and genetic toolkits. Rebollo E,Karkali K,Mangione F,Martin-Blanco E pmid: 24814031
    • 2016 - Following the Motion of Polycomb Bodies in Living Drosophila Embryos. Cheutin T,Cavalli G pmid: 27659993

    Molecular and protein biology basics

    web_pages

  • Alkaline lysis         ( http://www.bio-protocol.org/e30 )
  • Single fly PCR         ( http://theduroniolab.web.unc.edu/files/2013/10/Single-Fly-PCR.pdf )
  • Bradford assay         ( http://www.bio-protocol.org/e45 )
  • SDS-PAGE         ( http://www.bio-protocol.org/e80 )
    • publications

    • 2014 - Navigating and mining modENCODE data. Boley N,Wan KH,Bickel PJ,Celniker SE pmid: 24636835

    Omics approaches

    publications

  • 2008 - Purification of Drosophila protein complexes for mass spectrometry. Juschke C,Knoblich JA pmid: 18641959
  • 2014 - Enhanced Northern Blot detection of small RNA species in Drosophila melanogaster. Laneve P,Giangrande A pmid: 25177861
  • 2014 - Comparing mRNA levels using in situ hybridization of a target gene and co-stain. Wunderlich Z,Bragdon MD,DePace AH pmid: 24434507
  • 2015 - Combining protein and mRNA quantification to decipher transcriptional regulation. Xu H,Sepulveda LA,Figard L,Sokac AM,Golding I pmid: 26098021
  • 2015 - TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos. Bertin B,Renaud Y,Aradhya R,Jagla K,Junion G pmid: 26381166
  • 2013 - TALE-mediated modulation of transcriptional enhancers in vivo. Crocker J,Stern DL pmid: 23817068
  • 2013 - Building quantitative, three-dimensional atlases of gene expression and morphology at cellular resolution. Knowles DW,Biggin MD pmid: 24123936
  • 2014 - Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis. Ma J,Weake VM pmid: 24686501
  • 2012 - Transgenic expression and purification of myosin isoforms using the Drosophila melanogaster indirect flight muscle system. Caldwell JT,Melkani GC,Huxford T,Bernstein SI pmid: 22178692
  • 2013 - Assessment of transcript reconstruction methods for RNA-seq. Steijger T,Abril JF,Engstrom PG,Kokocinski F,Hubbard TJ,Guigo R,Harrow J,Bertone P pmid: 24185837
  • 2012 - Systematic evaluation of factors influencing ChIP-seq fidelity. Chen Y,Negre N,Li Q,Mieczkowska JO,Slattery M,Liu T,Zhang Y,Kim TK,He HH,Zieba J,Ruan Y,Bickel PJ,Myers RM,Wold BJ,White KP,Lieb JD,Liu XS pmid: 22522655
  • 2014 - Isolating intestinal stem cells from adult Drosophila midguts by FACS to study stem cell behavior during aging. Tauc HM,Tasdogan A,Pandur P pmid: 25548862
  • 2014 - Large-scale imaginal disc sorting: A protocol for "omics"-approaches. Marty F,Rockel-Bauer C,Simigdala N,Brunner E,Basler K pmid: 24736056
  • 2012 - Genome-wide localization of replication factors. Lubelsky Y,MacAlpine HK,MacAlpine DM pmid: 22465279
  • 2014 - An in vivo crosslinking approach to isolate protein complexes from Drosophila embryos. Gao M,McCluskey P,Loganathan SN,Arkov AL pmid: 24797807
  • 2014 - Mass spectrometry-driven phosphoproteomics: patterning the systems biology mosaic. Junger MA,Aebersold R pmid: 24902836
  • 2014 - High-resolution mapping of transcription factor binding sites on native chromatin. Kasinathan S,Orsi GA,Zentner GE,Ahmad K,Henikoff S pmid: 24336359
  • 2013 - Identifying RNA editing sites using RNA sequencing data alone. Ramaswami G,Zhang R,Piskol R,Keegan LP,Deng P,O'Connell MA,Li JB pmid: 23291724
  • 2015 - Detection of in situ protein-protein complexes at the Drosophila larval neuromuscular junction using proximity ligation assay. Wang S,Yoo S,Kim HY,Wang M,Zheng C,Parkhouse W,Krieger C,Harden N pmid: 25650626
  • 2013 - Identifying protein-protein interaction in Drosophila adult heads by Tandem Affinity Purification (TAP). Tian X,Zhu M,Li L,Wu C pmid: 24335807
  • 2014 - A user-friendly chromatographic method to purify small regulatory RNAs. Grentzinger T,Armenise C,Pelisson A,Brun C,Mugat B,Chambeyron S pmid: 23727218
  • 2013 - Enhanced dissection of the regulatory genome. Slattery M,White KP pmid: 23900252
  • web_pages

  • Protein extraction         ( http://www.bio-protocol.org/e1459 )
  • ChIP-on-chip         ( http://furlonglab.embl.de/methods/ )
  • ChIP chromatin assays         ( https://www.scientificprotocols.org/protocols/chromatin-preparation-an...... )
  • TAP         ( http://www.bio-protocol.org/e245 )
  • RNAseq         ( http://furlonglab.embl.de/methods/ )
  • Polytene chromosome in situ hybridization

    Protein activity assays

      publications

    • 2013 - A fluorescence-based exonuclease assay to characterize DmWRNexo, orthologue of human progeroid WRN exonuclease, and its application to other nucleases. Mason PA,Boubriak I,Cox LS pmid: 24378758

    Protein expression and purification

      publications

    • 2016 - Expression and Purification of Mitochondrial RNA Polymerase and Transcription Factor A from Drosophila melanogaster. Gajewski JP,Arnold JJ,Salminen TS,Kaguni LS,Cameron CE pmid: 26530684

    Proteomics

      publications

    • 2016 - Analysis of SUMOylated Proteins in Cells and In Vivo Using the bioSUMO Strategy. Pirone L,Xolalpa W,Mayor U,Barrio R,Sutherland JD pmid: 27631805

    Aging and longevity

    publications

  • 2016 - Protocols to Study Aging in Drosophila. Piper MD,Partridge L pmid: 27730590
  • 2014 - Studying aging in Drosophila. He Y,Jasper H pmid: 24751824
  • Apoptosis and autophagy

      publications

    • 2016 - Detection of Cell Death in Drosophila Tissues. Vasudevan D,Ryoo HD pmid: 27108437
    • 2015 - How and why to study autophagy in Drosophila: it's more than just a garbage chute. Nagy P,Varga A,Kovacs AL,Takats S,Juhasz G pmid: 25481477
    • 2008 - Experimental control and characterization of autophagy in Drosophila. Juhasz G,Neufeld TP pmid: 18425447
    • 2014 - Detecting apoptosis in Drosophila tissues and cells. Sarkissian T,Timmons A,Arya R,Abdelwahid E,White K pmid: 24613678
    • 2016 - Using Fluorescent Reporters to Monitor Autophagy in the Female Germline Cells in Drosophila melanogaster. Jacomin AC,Nezis IP pmid: 27557573
    • 2014 - Assays to monitor autophagy in Drosophila. Mauvezin C,Ayala C,Braden CR,Kim J,Neufeld TP pmid: 24667416
    • 2013 - Live imaging of apoptotic cell clearance during Drosophila embryogenesis. Shklyar B,Shklover J,Kurant E pmid: 23979068

    Behavior

    DIS techniques

  • The free-running period of Drosophila melanogaster is not affected by the length of the tube in a Drosophila Activity Monitor (DAM). Seggio, J.A.
  • Climbing assay. Klosowski, R., and B.T. Hovemann
  • The impact of pheromones on sexual behavior in D. melanogaster: Recommendations for laboratory protocols. Beck, A.P., E.F. Hamlin, E.L. Hume, and R.M. Hallock
  • Fluorescein dye as a tagging agent for Drosophila dispersal studies. Fingerut, J., K. Orbe, and S. McRobert
  • Assaying basal and ethanol-induced locomotion in flies using a custom built apparatus and freely available software Kliethermes, C.
  • publications

  • 2016 - Foraging Path-length Protocol for Drosophila melanogaster Larvae. Anreiter I,Vasquez OE,Allen AM,Sokolowski MB pmid: 27167330
  • 2015 - A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster. Murphy TP,Luu DD,DeSimone JA,O'Brien TC,Lally CJ,Lindblad JJ,Webster SM pmid: 26780205
  • 2016 - Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior. Sun X,Heckscher ES pmid: 27929468
  • 2016 - Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer. Lin CC,Riabinina O,Potter CJ pmid: 27585032
  • 2016 - A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster. Zer S,Ryvkin J,Wilner HJ,Zak H,Shmueli A,Shohat-Ophir G pmid: 28060352
  • 2016 - Protocols to Study Behavior in Drosophila. Neckameyer WS,Bhatt P pmid: 27730591
  • 2016 - Artificial Induction of Associative Olfactory Memory by Optogenetic and Thermogenetic Activation of Olfactory Sensory Neurons and Octopaminergic Neurons in Drosophila Larvae. Honda T,Lee CY,Honjo K,Furukubo-Tokunaga K pmid: 27445732
  • 2015 - A New Approach that Eliminates Handling for Studying Aggression and the "Loser" Effect in Drosophila melanogaster. Trannoy S,Chowdhury B,Kravitz EA pmid: 26780386
  • 2016 - High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster. Gou B,Zhu E,He R,Stern U,Yang CH pmid: 27077482
  • 2015 - Conditions Affecting Social Space in Drosophila melanogaster. McNeil AR,Jolley SN,Akinleye AA,Nurilov M,Rouzyi Z,Milunovich AJ,Chambers MC,Simon AF pmid: 26575105
  • 2014 - Straightforward assay for quantification of social avoidance in Drosophila melanogaster. Fernandez RW,Nurilov M,Feliciano O,McDonald IS,Simon AF pmid: 25549275
  • 2014 - The FlyBar: administering alcohol to flies. van der Linde K,Fumagalli E,Roman G,Lyons LC pmid: 24895004
  • 2013 - A single-fly assay for foraging behavior in Drosophila. Zaninovich OA,Kim SM,Root CR,Green DS,Ko KI,Wang JW pmid: 24299900
  • 2015 - An inexpensive, scalable behavioral assay for measuring ethanol sedation sensitivity and rapid tolerance in Drosophila. Sandhu S,Kollah AP,Lewellyn L,Chan RF,Grotewiel M pmid: 25939022
  • 2014 - Electrophysiological recording from Drosophila labellar taste sensilla. Delventhal R,Kiely A,Carlson JR pmid: 24638081
  • 2014 - Methods to characterize spontaneous and startle-induced locomotion in a rotenone-induced Parkinson's disease model of Drosophila. Liao J,Morin LW,Ahmad ST pmid: 25178101
  • 2013 - JAABA: interactive machine learning for automatic annotation of animal behavior. Kabra M,Robie AA,Rivera-Alba M,Branson S,Branson K pmid: 23202433
  • 2014 - FIM imaging and FIMtrack: two new tools allowing high-throughput and cost effective locomotion analysis. Risse B,Otto N,Berh D,Jiang X,Klambt C pmid: 25591081
  • 2014 - Testing Drosophila olfaction with a Y-maze assay. Simonnet MM,Berthelot-Grosjean M,Grosjean Y pmid: 24961243
  • 2013 - Functional analysis of the larval feeding circuit in Drosophila. Bhatt PK,Neckameyer WS pmid: 24300174
  • 2015 - Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders. Madabattula ST,Strautman JC,Bysice AM,O'Sullivan JA,Androschuk A,Rosenfelt C,Doucet K,Rouleau G,Bolduc F pmid: 26132637
  • 2014 - Determination of the spontaneous locomotor activity in Drosophila melanogaster. Woods JK,Kowalski S,Rogina B pmid: 24747955
  • 2014 - Acquisition of high-quality digital video of Drosophila larval and adult behaviors from a lateral perspective. Zenger B,Wetzel S,Duncan J pmid: 25350294
  • 2014 - idTracker: tracking individuals in a group by automatic identification of unmarked animals. Perez-Escudero A,Vicente-Page J,Hinz RC,Arganda S,de Polavieja GG pmid: 24880877
  • 2015 - Dyeing insects for behavioral assays: the mating behavior of anesthetized Drosophila. Verspoor RL,Heys C,Price TA pmid: 25938821
  • 2014 - A low-cost method for analyzing seizure-like activity and movement in Drosophila. Stone B,Burke B,Pathakamuri J,Coleman J,Kuebler D pmid: 24637378
  • 2014 - Drosophila adult olfactory shock learning. Malik BR,Hodge JJ pmid: 25145496
  • 2015 - A Novel Method for Tracking Individuals of Fruit Fly Swarms Flying in a Laboratory Flight Arena. Cheng XE,Qian ZM,Wang SH,Jiang N,Guo A,Chen YQ pmid: 26083385
  • videos

  • 2015 - A Novel Method for Tracking Individuals of Fruit Fly Swarms Flying in a Laboratory Flight Arena.         ( https://www.youtube.com/embed/9pL0TzjOchM )
  • Installation of software for sleep analysis         ( https://www.youtube.com/embed/videoseries?list=PL4774CD9BD372A130 )
  • Cell and tissue mechanics

      publications

    • 2016 - Laser Ablation to Probe the Epithelial Mechanics in Drosophila. Shivakumar PC,Lenne PF pmid: 27730586

    Cell cycle and division

      publications

    • 2016 - Model Organisms for Studying the Cell Cycle. Tang Z pmid: 26254916
    • 2016 - Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila Cells. Zielke N,van Straaten M,Bohlen J,Edgar BA pmid: 26254933

    Cell migration

      publications

    • 2016 - Live Imaging of Border Cell Migration in Drosophila. Dai W,Montell DJ pmid: 27271901

    Cells in tissues

      publications

    • 2014 - IV. Tools and methods for studying cell migration and cell rearrangement in tissue and organ development. Kanca O,Ochoa-Espinosa A,Affolter M pmid: 24631575

    Chromosomes

    publications

  • 2016 - Chromosome Conformation Capture in Drosophila. Li HB pmid: 27659987
  • 2016 - Rapid Ovary Mass-Isolation (ROMi) to Obtain Large Quantities of Drosophila Egg Chambers for Fluorescent In Situ Hybridization. Jambor H,Mejstrik P,Tomancak P pmid: 27730587
  • 2016 - Chromosome Conformation Capture on Chip (4C): Data Processing. Leblanc B,Comet I,Bantignies F,Cavalli G pmid: 27659990
  • 2016 - Chromatin Immunoprecipitation for Analyzing Transcription Factor Binding and Histone Modifications in Drosophila. Ghavi-Helm Y,Zhao B,Furlong EE pmid: 27730588
  • 2016 - Practical Analysis of Genome Contact Interaction Experiments. Carty MA,Elemento O pmid: 27008015
  • 2016 - Determination of Polycomb Group of Protein Compartmentalization Through Chromatin Fractionation Procedure. Marasca F,Marullo F,Lanzuolo C pmid: 27659984
  • 2016 - In Vivo Models to Address the Function of Polycomb Group Proteins. Bantignies F pmid: 27659991
  • 2016 - Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos. Loser E,Latreille D,Iovino N pmid: 27659972
  • 2012 - Chromosome conformation capture on chip in single Drosophila melanogaster tissues. Tolhuis B,Blom M,van Lohuizen M pmid: 22525789
  • Circadian rhythms

      publications

    • 2014 - Studying circadian rhythms in Drosophila melanogaster. Tataroglu O,Emery P pmid: 24412370
    • 2014 - Design and analysis of temperature preference behavior and its circadian rhythm in Drosophila. Goda T,Leslie JR,Hamada FN pmid: 24457268

    Cytoskeletal proteins

      publications

    • 2016 - Methods to Study Centrosomes and Cilia in Drosophila. Jana SC,Mendonca S,Werner S,Bettencourt-Dias M pmid: 27514925
    • 2016 - Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells. Applewhite DA,Davis CA,Griffis ER,Quintero OA pmid: 26498780

    Egg activation

      publications

    • 2016 - Imaging Calcium in Drosophila at Egg Activation. Derrick CJ,York-Andersen AH,Weil TT pmid: 27584955

    Embryogenesis

    publications

  • 2016 - Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos. Hauptmann G,Soll I,Krautz R,Theopold U pmid: 26862978
  • 2008 - Cuticle preparation of Drosophila embryos and larvae. Alexandre C pmid: 18641948
  • deGradFP: A System to Knockdown GFP-Tagged Proteins. Caussinus E,Affolter M pmid: 27730582
  • 2008 - Mass production of Drosophila embryos and chromatographic purification of native protein complexes. Kunert N,Brehm A pmid: 18641960
  • 2008 - Immunolabeling of embryos. Muller HA pmid: 18641949
  • 2016 - Performing Chromophore-Assisted Laser Inactivation in Drosophila Embryos Using GFP. Pelissier-Monier A,Sanson B,Monier B pmid: 27730580
  • 2008 - Extraction and immunoblotting of proteins from embryos. Wodarz A pmid: 18641958
  • 2016 - Live Staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands. Bali N,Lee HK,Zinn K pmid: 27514816
  • 2014 - A method of permeabilization of Drosophila embryos for assays of small molecule activity. Rand MD pmid: 25046169
  • 2008 - Imaging cellular and molecular dynamics in live embryos using fluorescent proteins. Cavey M,Lecuit T pmid: 18641950
  • 2007 - Fixation of Drosophila embryos. Rothwell WF,Sullivan W pmid: 21357167
  • 2015 - Tissue cartography: compressing bio-image data by dimensional reduction. Heemskerk I,Streichan SJ pmid: 26524242
  • 2007 - Propidium iodide staining of Drosophila embryos. Rothwell WF,Sullivan W pmid: 21356944
  • 2012 - Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy. Tomer R,Khairy K,Amat F,Keller PJ pmid: 22660741
  • 2007 - Collection, dechorionation, and preparation of Drosophila embryos for quantitative microinjection. Kiehart DP,Crawford JM,Montague RA pmid: 21357063
  • 2013 - Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos. Gisselbrecht SS,Barrera LA,Porsch M,Aboukhalil A,Estep PW 3rd,Vedenko A,Palagi A,Kim Y,Zhu X,Busser BW,Gamble CE,Iagovitina A,Singhania A,Michelson AM,Bulyk ML pmid: 23852450
  • 2013 - Image analysis and empirical modeling of gene and protein expression. Trisnadi N,Altinok A,Stathopoulos A,Reeves GT pmid: 23104159
  • 2007 - DAPI Staining of Drosophila Embryos. Rothwell WF,Sullivan W pmid: 21356945
  • 2015 - Whole-animal functional and developmental imaging with isotropic spatial resolution. Chhetri RK,Amat F,Wan Y,Hockendorf B,Lemon WC,Keller PJ pmid: 26501515
  • videos

  • WingJ: unsupervised segmentation of the Drosophila embryo         ( https://www.youtube.com/embed/othDMRkjhAg )
  • web_pages

  • BrdU labeling of ovaries         ( http://theduroniolab.web.unc.edu/files/2013/10/BrdU-on-ovaries-Pelin-C...... )
  • in situ hybridization of embryos         ( http://theduroniolab.web.unc.edu/files/2013/10/Embryo-in-situ.pdf )
  • Fixation of embryos         ( http://theduroniolab.web.unc.edu/files/2013/10/Embryo-Fixation.pdf )
  • Eye development

      publications

    • 2008 - Wingless signaling in Drosophila eye development. Legent K,Treisman JE pmid: 19109709
    • 2014 - Eye development. Baker NE,Li K,Quiquand M,Ruggiero R,Wang LH pmid: 24784530
    • 2015 - Live-imaging of the Drosophila pupal eye. Hellerman MB,Choe RH,Johnson RI pmid: 25651413
    • 2013 - The Tomato/GFP-FLP/FRT method for live imaging of mosaic adult Drosophila photoreceptor cells. Dourlen P,Levet C,Mejat A,Gambis A,Mollereau B pmid: 24084155
    • 2008 - Microscopic analysis of the adult Drosophila retina using semithin plastic sections. Gaengel K,Mlodzik M pmid: 18641954
    • 2014 - Dissection and mounting of Drosophila pupal eye discs. Tea JS,Cespedes A,Dawson D,Banerjee U,Call GB pmid: 25406645

    Flight

      publications

    • 2014 - An improved method for accurate and rapid measurement of flight performance in Drosophila. Babcock DT,Ganetzky B pmid: 24561810

    Gene expression

      publications

    • 2016 - Data analysis algorithm for DamID-seq profiling of chromatin proteins in Drosophila melanogaster. Maksimov DA,Laktionov PP,Belyakin SN pmid: 27766446

    web_pages

  • Gene Expression Analysis of Sorted Cells by RNA-seq in Drosophila Intestine         ( http://www.bio-protocol.org/e2079 )
  • Growth and metabolism

      publications

    • 2016 - Protocols to Study Growth and Metabolism in Drosophila. Strassburger K,Teleman AA pmid: 27730589

    Heart biology

      publications

    • 2012 - A novel method for obtaining semi-thin cross sections of the Drosophila heart and their labeling with multiple antibodies. Harpaz N,Volk T pmid: 21963658
    • 2012 - Purification of cardiac cells from Drosophila embryos. Bryantsev AL,Cripps RM pmid: 22119843
    • 2014 - Methods to assess Drosophila heart development, function and aging. Ocorr K,Vogler G,Bodmer R pmid: 24727147

    Hematopoiesis

      publications

    • 2014 - Drosophila hematopoiesis: Markers and methods for molecular genetic analysis. Evans CJ,Liu T,Banerjee U pmid: 24613936
    • 2015 - Assaying Blood Cell Populations of the Drosophila melanogaster Larva. Petraki S,Alexander B,Bruckner K pmid: 26650404

    Imaginal discs

      publications

    • 2016 - Imaginal Disc Transplantation in Drosophila. Katsuyama T,Paro R pmid: 27659995
    • 2008 - Immunolabeling of imaginal discs. Klein T pmid: 18641952
    • 2016 - Cultivation and Live Imaging of Drosophila Imaginal Discs. Restrepo S,Zartman JJ,Basler K pmid: 27730583
    • 2008 - Imaging Drosophila pupal wing morphogenesis. Classen AK,Aigouy B,Giangrande A,Eaton S pmid: 18641953
    • 2014 - Dissection and immunostaining of imaginal discs from Drosophila melanogaster. Spratford CM,Kumar JP pmid: 25285379
    • 2007 - Dissection of imaginal discs in Drosophila. Blair SS pmid: 21356988

    web_pages

  • Colbalt staining of eye imaginal discs         ( http://theduroniolab.web.unc.edu/files/2013/10/Cobalt-staining-of-eye-...... )
  • BrdU labeling of imaginal discs         ( http://theduroniolab.web.unc.edu/files/2013/10/Eye-disc-BrdU.pdf )
  • FACS analysis of wing disc         ( http://theduroniolab.web.unc.edu/files/2013/10/FACS-Analysis-of-Wing-D...... )
  • Immunity and immflamation

      publications

    • 2011 - RNAi screening for host factors involved in viral infection using Drosophila cells. Cherry S pmid: 21431698
    • 2014 - Methods to study Drosophila immunity. Neyen C,Bretscher AJ,Binggeli O,Lemaitre B pmid: 24631888
    • 2013 - Novel whole-tissue quantitative assay of nitric oxide levels in Drosophila neuroinflammatory response. Ajjuri RR,O'Donnell JM pmid: 24335747
    • 2015 - Systemic bacterial infection and immune defense phenotypes in Drosophila melanogaster. Khalil S,Jacobson E,Chambers MC,Lazzaro BP pmid: 25992475

    Injury responses

      publications

    • 2015 - A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila. Katzenberger RJ,Loewen CA,Bockstruck RT,Woods MA,Ganetzky B,Wassarman DA pmid: 26168076
    • 2013 - Microinjection wound assay and in vivo localization of epidermal wound response reporters in Drosophila embryos. Juarez MT,Patterson RA,Li W,McGinnis W pmid: 24300796
    • 2014 - Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae. Mishra B,Ghannad-Rezaie M,Li J,Wang X,Hao Y,Ye B,Chronis N,Collins CA pmid: 24562098

    Larvae

      publications

    • 2014 - Sonication-facilitated immunofluorescence staining of late-stage embryonic and larval Drosophila tissues in situ. Fidler A,Boulay L,Wawersik M pmid: 25146311
    • 2015 - Measurement of larval activity in the Drosophila activity monitor. McParland AL,Follansbee TL,Ganter GK pmid: 25993121

    videos

  • Drosophila larval third instar CNS dissection: slow         ( https://www.youtube.com/embed/9WlIoxxFuy0 )
  • web_pages

  • BrdU labeling of larvae         ( http://theduroniolab.web.unc.edu/files/2013/10/BrdU-labeling-whole-lar...... )
  • in situ hybridization of larvae         ( http://theduroniolab.web.unc.edu/files/2013/10/Larval-in-situs.pdf )
  • Malphigian tubules

      publications

    • 2015 - Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule. Schellinger JN,Rodan AR pmid: 26650886

    Metabolism

      publications

    • 2017 - Staining of Lipid Droplets with Monodansylpentane. Chen BH,Yang HJ,Chou HY,Chen GC,Yang WY pmid: 28155158
    • 2014 - Methods for studying metabolism in Drosophila. Tennessen JM,Barry WE,Cox J,Thummel CS pmid: 24631891
    • 2015 - Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila. Villa-Cuesta E,Rand DM pmid: 26485391
    • 2014 - Measurement of metabolic rate in Drosophila using respirometry. Yatsenko AS,Marrone AK,Kucherenko MM,Shcherbata HR pmid: 24998593

    videos

  • 2012 - Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila.         ( https://www.youtube.com/embed/C5-HKFFxtoI?list=PLBXEefaOGKebpBonK6m_Xl...... )
  • web_pages

  • Fat Turnover Assay in Drosophila         ( http://www.bio-protocol.org/e1996 )
  • MiRNAs

      publications

    • 2016 - Analysis of MicroRNA Function in Drosophila. Chawla G,Luhur A,Sokol N pmid: 27730576
    • 2008 - An overview of the identification, detection, and functional analysis of Drosophila microRNAs. Sokol NS pmid: 18641957

    Mitochondria

      publications

    • 2016 - In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining. Chen Z,Xu H pmid: 27805603

    Muscle biology

      publications

    • 2014 - A guide to study Drosophila muscle biology. Weitkunat M,Schnorrer F pmid: 24625467
    • 2016 - Imaging Approaches to Investigate Myonuclear Positioning in Drosophila. Azevedo M,Schulman VK,Folker E,Balakrishnan M,Baylies M pmid: 27147050
    • 2008 - Analysis of neuromuscular junctions: histology and in vivo imaging. Schmid A,Sigrist SJ pmid: 18641951
    • 2015 - Live imaging of muscles in Drosophila metamorphosis: Towards high-throughput gene identification and function analysis. Puah WC,Wasser M pmid: 26431669
    • 2008 - Myoblast fusion in Drosophila. Abmayr SM,Zhuang S,Geisbrecht ER pmid: 18979239
    • 2012 - Courtship song analysis of Drosophila muscle mutants. Chakravorty S,Wajda MP,Vigoreaux JO pmid: 21945578
    • 2008 - Live imaging of Drosophila myoblast fusion. Richardson BE,Beckett K,Baylies MK pmid: 18979249
    • 2012 - In vivo measurement of muscle output in intact Drosophila. Elliott CJ,Sparrow JC pmid: 22037247
    • 2008 - Ultrastructural analysis of myoblast fusion in Drosophila. Zhang S,Chen EH pmid: 18979250
    • 2012 - Recent advances in imaging embryonic myoblast fusion in Drosophila. Haralalka S,Cartwright HN,Abmayr SM pmid: 21871963
    • 2012 - Mechanical analysis of Drosophila indirect flight and jump muscles. Swank DM pmid: 22079350
    • 2008 - A genomic approach to myoblast fusion in Drosophila. Estrada B,Michelson AM pmid: 18979251

    Neurobiology

    publications

  • 2016 - Electrophysiological Recordings from Lobula Plate Tangential Cells in Drosophila. Mauss AS,Borst A pmid: 27730592
  • 2016 - Optogenetic Manipulation of Selective Neural Activity in Free-Moving Drosophila Adults. Hsiao PY,Wu MC,Lin YY,Fu CC,Chiang AS pmid: 26965137
  • 2016 - Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila. He Q,Roblodowski C pmid: 26498796
  • 2016 - A simple method for imaging axonal transport in aging neurons using the adult Drosophila wing. Vagnoni A,Bullock SL pmid: 27560175
  • 2016 - Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells. Tenenbaum CM,Gavis ER pmid: 27842373
  • 2016 - Optogenetics in Drosophila Neuroscience. Riemensperger T,Kittel RJ,Fiala A pmid: 26965122
  • 2016 - Laser Desorption Ionization Mass Spectrometry Imaging of Drosophila Brain Using Matrix Sublimation versus Modification with Nanoparticles. Phan NT,Mohammadi AS,Dowlatshahi Pour M,Ewing AG pmid: 26705612
  • 2013 - Initial neurogenesis in Drosophila. Hartenstein V,Wodarz A pmid: 24014455
  • 2014 - Independent optical excitation of distinct neural populations. Klapoetke NC,Murata Y,Kim SS,Pulver SR,Birdsey-Benson A,Cho YK,Morimoto TK,Chuong AS,Carpenter EJ,Tian Z,Wang J,Xie Y,Yan Z,Zhang Y,Chow BY,Surek B,Melkonian M,Jayaraman V,Constantine-Paton M,Wong GK,Boyden ES pmid: 24509633
  • 2014 - Live imaging of Drosophila larval neuroblasts. Lerit DA,Plevock KM,Rusan NM pmid: 25046336
  • 2014 - FlyMAD: rapid thermogenetic control of neuronal activity in freely walking Drosophila. Bath DE,Stowers JR,Hormann D,Poehlmann A,Dickson BJ,Straw AD pmid: 24859752
  • 2011 - Flybow: genetic multicolor cell labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D,Rotkopf S,Alexandre C,Bell DM,Dickson BJ,Salecker I pmid: 21297619
  • 2015 - Versatile genetic paintbrushes: Brainbow technologies. Richier B,Salecker I pmid: 25491327
  • 2015 - Whole-central nervous system functional imaging in larval Drosophila. Lemon WC,Pulver SR,Hockendorf B,McDole K,Branson K,Freeman J,Keller PJ pmid: 26263051
  • 2014 - Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship. Inagaki HK,Jung Y,Hoopfer ED,Wong AM,Mishra N,Lin JY,Tsien RY,Anderson DJ pmid: 24363022
  • 2006 - A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining. Wu JS,Luo L pmid: 17487202
  • 2015 - Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Berlin S,Carroll EC,Newman ZL,Okada HO,Quinn CM,Kallman B,Rockwell NC,Martin SS,Lagarias JC,Isacoff EY pmid: 26167640
  • 2011 - Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural expression patterns. Hampel S,Chung P,McKellar CE,Hall D,Looger LL,Simpson JH pmid: 21297621
  • 2014 - Whole mount immunolabeling of olfactory receptor neurons in the Drosophila antenna. Karim MR,Endo K,Moore AW,Taniguchi H pmid: 24834898
  • 2014 - Fast, accurate reconstruction of cell lineages from large-scale fluorescence microscopy data. Amat F,Lemon W,Mossing DP,McDole K,Wan Y,Branson K,Myers EW,Keller PJ pmid: 25042785
  • videos

  • 2014 - FlyMAD: rapid thermogenetic control of neuronal activity in freely walking Drosophila.         ( https://www.youtube.com/embed/SDIEBgtSSJk )
  • Dissection of an Adult Brain Drosophila         ( https://www.youtube.com/embed/aGcnJeqEVEk )
  • 2015 - Whole-central nervous system functional imaging in larval Drosophila.         ( https://www.youtube.com/embed/Msf-q2gKsug )
  • 2006 - A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining.         ( https://www.youtube.com/embed/j4rVa7JCzdg )
  • Dissection an Adult Brain of Drosophila         ( https://www.youtube.com/embed/aGcnJeqEVEk )
  • web_pages

  • Electroretinogram (ERG) Recordings from Drosophila         ( http://www.bio-protocol.org/e1636 )
  • GABA staining of Drosophila brain         ( https://www.google.com/url?q=https://www.ncbs.res.in/sitefiles/GABA%25...... )
  • Electroretinography         ( http://www.bio-protocol.org/e1636 )
  • Oogenesis

      publications

    • 2016 - In Vitro Culturing and Live Imaging of Drosophila Egg Chambers: A History and Adaptable Method. Peters NC,Berg CA pmid: 27557572
    • 2016 - Immuno-Gold Labeling of Drosophila Follicles for Transmission Electron Microscopy. Tsapras P,Sagona AP,Nezis IP pmid: 27557575
    • 2016 - Cultivation and Live Imaging of Drosophila Ovaries. Cetera M,Lewellyn L,Horne-Badovinac S pmid: 27730584
    • 2016 - Preparation of Drosophila Follicles for Transmission Electron Microscopy. Palara A,Sagona AP,Nezis IP pmid: 27557576
    • 2016 - Antibody Staining in Drosophila Germaria. Lie-Jensen A,Haglund K pmid: 27557571
    • 2014 - Methods for studying oogenesis. Hudson AM,Cooley L pmid: 24440745
    • 2016 - Analysis of Phagocytosis in the Drosophila Ovary. Meehan TL,Serizier SB,Kleinsorge SE,McCall K pmid: 27557574
    • 2013 - The utility of stage-specific mid-to-late Drosophila follicle isolation. Spracklen AJ,Tootle TL pmid: 24326735
    • 2015 - Upright imaging of Drosophila egg chambers. Manning L,Starz-Gaiano M pmid: 25867882

    videos

  • Drosophila Ovary Dissection         ( https://www.youtube.com/embed/T94be2i5qb4 )
  • Drosophila Ovarian Dissection for Immunohistochemistry         ( https://www.youtube.com/embed/oYzjE_zqc1w )
  • web_pages

  • Follicle cell isolation from ovaries         ( http://theduroniolab.web.unc.edu/files/2013/10/Follicle-Cell-Iso-Proto...... )
  • Physiology and nutrition

      publications

    • 2014 - Quantifying Drosophila food intake: comparative analysis of current methodology. Deshpande SA,Carvalho GB,Amador A,Phillips AM,Hoxha S,Lizotte KJ,Ja WW pmid: 24681694
    • 2014 - A holidic medium for Drosophila melanogaster. Piper MD,Blanc E,Leitao-Goncalves R,Yang M,He X,Linford NJ,Hoddinott MP,Hopfen C,Soultoukis GA,Niemeyer C,Kerr F,Pletcher SD,Ribeiro C,Partridge L pmid: 24240321

    web_pages

  • Holidic media         ( https://www.scientificprotocols.org/protocols/preparation-of-a-holidic...... )
  • Post-translational modifications

      publications

    • 2016 - Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells. Astray RM,Suarez-Patino SF,Jorge SA pmid: 27485332

    Pupae

      publications

    • 2014 - Imaging through the pupal case of Drosophila melanogaster. Keroles MB,Dusseault SK,Liu C,Mohammed MR,Vadakkan CM,Amiel JH,Abel SN,Bensoussan ER,Russell BL,Baker J pmid: 24513575

    RNA editing

      publications

    • 2016 - Genome-Wide Analysis of A-to-I RNA Editing. Savva YA,Laurent GS,Reenan RA pmid: 26463388

    Salivary glands

    Signal transduction

    publications

  • 2017 - Co-culture Activation of MAP Kinase in Drosophila S2 Cells. Steinhauer J pmid: 27924571
  • 2014 - Modulating and measuring Wingless signalling. Vincent JP pmid: 24675402
  • 2008 - Visualization of PCP defects in the eye and wing of Drosophila melanogaster. Arbouzova N,McNeill H pmid: 19109708
  • 2014 - Tools and methods for studying the Drosophila JAK/STAT pathway. Chen Q,Giedt M,Tang L,Harrison DA pmid: 24685392
  • 2014 - The regulation and functions of MAPK pathways in Drosophila. Shilo BZ pmid: 24530508
  • 2008 - Proteomic analyses of protein complexes in the Wnt pathway. Angers S pmid: 19099258
  • 2014 - Strategies for exploring TGF-beta signaling in Drosophila. Peterson AJ,O'Connor MB pmid: 24680699
  • 2014 - Tools and methods for studying Notch signaling in Drosophila melanogaster. Zacharioudaki E,Bray SJ pmid: 24704358
  • 2014 - Wing tips: The wing disc as a platform for studying Hedgehog signaling. Hartl TA,Scott MP pmid: 24556557
  • 2016 - Genome-Wide RNAi Screening to Dissect the TGF-beta Signal Transduction Pathway. Chen X,Xu L pmid: 26520138
  • 2008 - Function of the wingless signaling pathway in Drosophila. Gonsalves FC,DasGupta R pmid: 19109707
  • 2014 - Methods for studying planar cell polarity. Olofsson J,Axelrod JD pmid: 24680701
  • 2014 - Immunohistochemical tools and techniques to visualize Notch in Drosophila melanogaster. Tognon E,Vaccari T pmid: 25053481
  • 2014 - Structure-function analysis of Drosophila Notch using genomic rescue transgenes. Leonardi J,Jafar-Nejad H pmid: 25053479
  • 2014 - Visualizing Notch signaling in vivo in Drosophila tissues. Housden BE,Li J,Bray SJ pmid: 25053484
  • Spermatogenesis

    publications

  • 2017 - Visualization of cleavage furrow proteins in fixed dividing spermatocytes. Frappaolo A,Sechi S,Belloni G,Piergentili R,Giansanti MG pmid: 28065322
  • 2014 - Genetics of male fertility. Lin YN,Matzuk MM pmid: 24782004
  • 2014 - Cytological analysis of spermatogenesis: live and fixed preparations of Drosophila testes. Sitaram P,Hainline SG,Lee LA pmid: 24473184
  • 2013 - Assessing differences in sperm competitive ability in Drosophila. Yeh SD,Chan C,Ranz JM pmid: 23995693
  • 2014 - Ex vivo culture of Drosophila pupal testis and single male germ-line cysts: dissection, imaging, and pharmacological treatment. Gartner SM,Rathke C,Renkawitz-Pohl R,Awe S pmid: 25286189
  • 2013 - Imaging centrosomes in fly testes. Basiri ML,Blachon S,Chim YC,Avidor-Reiss T pmid: 24084634
  • 2014 - Investigating spermatogenesis in Drosophila melanogaster. Demarco RS,Eikenes AH,Haglund K,Jones DL pmid: 24798812
  • videos

  • Drosophila Melanogaster Spermatocyte Dissection         ( https://www.youtube.com/embed/-ej8nF1YsRg?list=PLBXEefaOGKebpBonK6m_Xl...... )
  • web_pages

  • in situ hybridization of testes         ( http://theduroniolab.web.unc.edu/files/2013/10/Testis-in-situ.pdf )
  • Stem cells

      publications

    • 2012 - Drosophila models of epithelial stem cells and their niches. Sahai-Hernandez P,Castanieto A,Nystul TG pmid: 23801493

    Stress responses

      publications

    • 2014 - Analysis of translation initiation during stress conditions by polysome profiling. Coudert L,Adjibade P,Mazroui R pmid: 24893838

    The brain

      publications

    • 2016 - A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains. Tito AJ,Cheema S,Jiang M,Zhang S pmid: 27929474

    The gastrointestinal tract

      publications

    • 2014 - Whole-mount immunostaining of the adult Drosophila gastrointestinal tract. Micchelli CA pmid: 24680702
    • 2016 - Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the Drosophila Intestine. Luo L,Reedy AR,Jones RM pmid: 27246026

    The heart

      publications

    • 2016 - Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM). Men J,Jerwick J,Wu P,Chen M,Alex A,Ma Y,Tanzi RE,Li A,Zhou C pmid: 28060288

    The tracheal system

    Tumors and tumor induction

      publications

    • 2016 - The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics. Mundorf J,Uhlirova M pmid: 27768082
    • 2013 - A protocol for genetic induction and visualization of benign and invasive tumors in cephalic complexes of Drosophila melanogaster. Srivastava A pmid: 24056923
    • 2015 - Studying tumor growth in Drosophila using the tissue allograft method. Rossi F,Gonzalez C pmid: 26357008

    Vesicle transport

      publications

    • 2015 - Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics. Schneider J,Zahn J,Maglione M,Sigrist SJ,Marquard J,Chojnacki J,Krausslich HG,Sahl SJ,Engelhardt J,Hell SW pmid: 26214129

    Transgenesis