CRIMIC CRISPR MiMIC Gene Trap Method

For genes that permit intronic tagging, we design homology arms within the 5’-most intron that can be used to tag all or most of the predicted splice isoforms. These homology arms are cloned into a donor vector such that they flank a mutagenic CRISPR MiMIC (CRIMIC) gene trap, containing a splice acceptor, stop codons, as well as a T2A-Gal4. Injection of the construct and an sgRNA targeting the intron into Cas9-expressing fly embryos induces a DNA double-strand break in the germline, which allows for integration of the CRIMIC trap by homology directed repair. Once integrated, the CRIMIC insert produces a truncated mRNA of the target gene, as well as Gal4 under the control of the endogenous gene regulatory elements. These cassettes can then be converted into protein traps using established RMCE methods.


Nominate Gene List

Before submitting your nomination, please check the MiMIC database to verify if your gene does not already have a T2A-Gal4 line or a MiMIC insertion suitable for Recombination Mediated Cassette Exchange (RMCE).

Gene List*

Use this template to upload your gene list:

We ask that you try to limit your nomination to 10 genes or less. It can be more, but the project manager may contact you for more details.

NOTE: FBgn is required but gene annotation (CG) and symbol can be left blank. Any rows with missing FBgns will not be nominated.

*required fields


Clear

Internal CRIMIC Portal

To check nomination status, injection status, and more, login here.

Contact Us

If you have any questions or concerns regarding your nominations, contact Jonathan at jonathan_zirin@hms.harvard.edu.