RNAi Stock Validation & Phenotypes

P{TRiP.HMS00818}attP2

Gene Information TRiP Stock Information hide
Symbol:Nedd8
CG:CG10679
FBgn: FBgn0032725
Source: TRiP Line ID: HMS00818 Collection: RNAi
BDSC ID: 33881
Vector: VALIUM20 Insertion Site: attP2
Validation Test Results hide
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Category: agametic
  • Phenotype: no eggs
  • Penetrance: 100%
  • Temperature (°C): 29
Driver:w; matalpha4-GAL-VP16
Driver BDSC: 7063
Contributor(s): Lynn Cooley and Kathleen Ayers, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Staining info: Alexa 488-phalloidin (Molecular Probes) and DAPI (Molecular Probes)
  • Penetrance: 100%
  • Ovary Phenotype: degeneration stage 10
  • Female Fertility: eggs laid, no progeny
  • Temperature (°C): 25
 Image Link
Driver:Act5C-Gal4
Driver BDSC: not annotated
Contributor(s): Zeng et al., 2015
Reference: Zeng et al., 2015 PubMed Entry
Phenotype Results:
  • Phenotype: lethal
  • Temperature (°C): 25
Driver:nubbin-Gal4
Driver BDSC: not annotated
Contributor(s): Raquel A. Oliveira
Reference: Silva RD, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: wing phenotype
  • Phenotype: Lethal when tested in combination with san RNAi
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: The average mitochondrial length was longer than that in the wild type (about 2 um), and diameter larger than 2 um (about five times of the average diameter of the mitochondrion in the wild-type fat body tissues)
  • Penetrance: 3.46%
  • Temperature (°C): 25
Driver:VP16.mat.?Tub67C
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Hudson AM, et al., 2015 PubMed Entry
Phenotype Results:
  • Phenotype_1: GO0015629:actin cytoskeleton | adult stage | female, with Scer\GAL4[VP16.mat.?Tub67C]
  • Phenotype: nurse cell ring canal, with Scer\GAL4[VP16.mat.?Tub67C]
  • FlyBase Allele ID: FBal0257499
Driver:Act5C.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Liu Y, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_1: male germline stem cell | adult stage, with Scer\GAL4[Act5C.PU]
  • Phenotype: testis | adult stage, with Scer\GAL4[Act5C.PU]
  • FlyBase Allele ID: FBal0257499
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Temperature*: °C
qPCR data: % Remaining after knockdown
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