The Transgenic RNAi Resource Project

HOME

ABOUT

APPROACH

TRiP STOCKS

TRiP REAGENTS

FAQ

CONTACT

DRSC

RSVP

T R i P N E W S:

Search our Database

TRiP stock list updated April 4, 2013. NEW: We've added a column annotating the chromosome arm of the targeted gene. All hairpin sequences have been analyzed and re-annotated in accordance with FlyBase release: FlyBase release: r5.47 (FB2012_05).
We've refreshed our vector maps. Please download pdfs from the links below or the Reagents page. All TRiP vector requests should now be directed to the DF/HCC DNA Resource Core. See Reagent page for more information.
Try the Batch Search Tool to find available TRiP stocks for all of your favorite genes in one go!
More than 6000 TRiP lines are now available to order from the BDSC.

ABOUT the TRiP

TRiP STOCK LISTS

DESIGN APPROACH

Insertion Site Strategy
Injection
First Generation Hairpins - long double-stranded RNA in VALIUM1 and VALIUM10
Second Generation Hairpins - short hairpin RNA in VALIUM20 and VALIUM22 (and VALIUM21)

REAGENTS, MAPS & PROTOCOLS
Knockdown Vectors: pVALIUM1 pVALIUM10 pWALIUM10 pVALIUM20 pVALIUM22 pWALIUM20 pWALIUM22	Overexpression Vectors: pVALIUM10-roe pVALIUM10-moe pWALIUM10-roe pWALIUM10-moe	Cloning Tools: mENTRY

If use of any of the TRiP reagents results in data that is included in a manuscript for publication, the TRiP requests that a version of the following statement be included in the Acknowledgements section: "We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing transgenic RNAi fly stocks and/or plasmid vectors used in this study."

The Transgenic RNAi Project at Harvard Medical School 77 Avenue Louis Pasteur - NRB 336 - Boston, MA 02115