RNAi Stock Validation & Phenotypes

P{TRiP.HMS00044}attP2

Gene Information TRiP Stock Information hide
Symbol:Pten
CG:CG5671
FBgn: FBgn0026379
Source: TRiP Line ID: HMS00044 Collection: RNAi
BDSC ID: 33643
Vector: VALIUM20 Insertion Site: attP2
Validation Test Results hide
Driver:y w; Dmef2.R-Gal4
Driver BDSC: 27390
Contributor(s): Perrimon and Randklev, 2013, unpublished data.
Phenotype Results:
  • Phenotype: no phenotype
  • Temperature (°C): 27
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype: some embryonic lethal
  • Temperature (°C): 29
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Richelle Sopko and TRiP, 2014
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • qPCR reference gene: rp49/gapdh/tubulin/nuf
  • Phenotype Category: non-maternal effect
  • Female Fertility: 77% hatching
  • Temperature (°C): 29
  • qPCR % Transcript Remaining: 5.0%
Driver:Act5C-Gal4
Driver BDSC: not annotated
Contributor(s): Zeng et al., 2015
Reference: Zeng et al., 2015 PubMed Entry
Phenotype Results:
  • Phenotype: lethal
  • Temperature (°C): 25
Driver:eya composite-GAL4
Driver BDSC: not annotated
Contributor(s): Jason Tennessen
Reference: supplemental table 2, Fletcher et al, 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Eye phenotype
  • Phenotype: rough
  • Penetrance: 38% (total number=13)
  • Temperature (°C): 25
Driver:eya composite-GAL4
Driver BDSC: not annotated
Contributor(s): Jason Tennessen
Reference: supplemental table 3, Fletcher et al, 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Eye phenotype
  • Phenotype: overgrowth
  • Penetrance: 100% (100% of 40 male; 100% of 20 female)
  • Temperature (°C): 25
Driver:nubbin-Gal4
Driver BDSC: not annotated
Contributor(s): Raquel A. Oliveira
Reference: Silva RD, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: wing phenotype
  • Phenotype: Enhanced wing morphological defects with san RNAi
Driver:c587-gal4; nos-gal4
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Ovary phenotype
  • Phenotype: larval or pupal lethal
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: No phenotype
  • Temperature (°C): 25
Driver:eya.enh1+E+2
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: supplemental table 2, Fletcher et al, 2019 PubMed Entry
Phenotype Results:
  • Phenotype_1: size defective | adult stage, with Scer\GAL4[eya.enh1+E+2]
  • Phenotype: eye, with Scer\GAL4[eya.enh1+E+2]
  • FlyBase Allele ID: FBal0257084
Driver:pnr-MD237
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Urwyler O, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype_4: GO0043005:neuron projection | adult stage, with Scer\GAL4[pnr-MD237]
  • Phenotype_3: neuroanatomy defective | adult stage, with Scer\GAL4[pnr-MD237]
  • Phenotype_2: size defective | adult stage, with Scer\GAL4[pnr-MD237]
  • Phenotype_1: mechanosensory neuron of posterior dorsocentral bristle, with Scer\GAL4[pnr-MD237]
  • Phenotype: synapse | adult stage, with Scer\GAL4[pnr-MD237]
  • FlyBase Allele ID: FBal0257084
Driver:nos.PG, VP16.nos.UTR, VP16.otu
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype: female semi-sterile, with Scer\GAL4[nos.PG], Scer\GAL4[VP16.nos.UTR], Scer\GAL4[VP16.otu]
  • FlyBase Allele ID: FBal0257084
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Temperature*: °C
qPCR data: % Remaining after knockdown
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