RNAi Stock Validation & Phenotypes

P{TRiP.GL00069}attP2

Gene Information TRiP Stock Information hide
Symbol:put
CG:CG7904
FBgn: FBgn0003169
Source: TRiP Line ID: GL00069 Collection: RNAi
BDSC ID: 35195
Vector: VALIUM22 Insertion Site: attP2
Validation Test Results hide
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Category: stem cell loss
  • Phenotype Antibody Info: a-Spectrin antobody is from DSHB, 3A9; mouse Vasa antibody is from Santa Cruz Biotechnology, d-260,
  • Phenotype: first day has normal amount of embryos, then drop to very few
  • Temperature (°C): 29
Driver:P{mat-tub-GAL4}2318
Driver BDSC: not annotated
Contributor(s): Richelle Sopko and TRiP, 2014
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • qPCR reference gene: nuf/rp49
  • Phenotype Description: ventralized phenotype, then no eggs
  • Phenotype Category: maternal effect; oogenesis defect
  • Phenotype: D/V defects; no egg
  • Female Fertility: 0% hatching
  • Temperature (°C): 29
  • qPCR % Transcript Remaining: 12.0%
 Image Link
Driver:VP16.mat.?Tub67C
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype_1: female sterile, with Scer\GAL4[VP16.mat.?Tub67C]
  • Phenotype: embryo | maternal effect, with Scer\GAL4[VP16.mat.?Tub67C]
  • FlyBase Allele ID: FBal0262556
Driver:NP0261
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Redhai S, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_1: GO0031045:dense core granule | adult stage, with Scer\GAL4[NP0261]
  • Phenotype: male accessory gland secondary cell, with Scer\GAL4[NP0261]
  • FlyBase Allele ID: FBal0262556
Driver:dsx.KI
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Redhai S, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_4: GO0099503:secretory vesicle | adult stage, with Scer\GAL4[dsx.KI]
  • Phenotype_3: GO0005771:multivesicular body | adult stage, with Scer\GAL4[dsx.KI]
  • Phenotype_2: GO0005764:lysosome | adult stage, with Scer\GAL4[dsx.KI]
  • Phenotype_1: size defective | adult stage, with Scer\GAL4[dsx.KI]
  • Phenotype: male accessory gland secondary cell, with Scer\GAL4[dsx.KI]
  • FlyBase Allele ID: FBal0262556
Driver:esg.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Redhai S, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_2: GO0005634:nucleus | adult stage, with Scer\GAL4[esg.PU]
  • Phenotype_1: size defective | adult stage, with Scer\GAL4[esg.PU]
  • Phenotype: male accessory gland secondary cell, with Scer\GAL4[esg.PU]
  • FlyBase Allele ID: FBal0262556
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Temperature*: °C
qPCR data: % Remaining after knockdown
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