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Are you interested in targeting SNP or INDEL variants in your experiment? Look no further! We can help you find CRISPR guide designs (both -NGG and -NAG) in Fly, Human, Mouse, Zebrafish, and Rat genomes. Each guide will be given an efficiency and off-target score based on our algorithms so that you can plan accordingly. Targeting multiple variants within the same guide is also available (select the checkbox)!

Upload a .vcf or .csv file of 2000 or less variants (sample files here) -- listed alleles can either be uppercase or lowercase.