New Template files (May 2019). Download the new templates before begining
- If you only have the
gene information?, download this template:
- If you have both
gene and gRNA information, download this template:
- If you have gene, gRNA, and primer information, download this template:
genetics.med.harvard.edu) with any questions.
TRiP-KO stocks will now be made using the pCFD6 vector, in which two guides are expressed under the control of a UAS promoter. These stocks are suitable for both somatic and germline KO. Please contact Jonathan Zirin (jzirin@
TRiP-OE stocks are now being made in the flySAM2.0 (VTPHG) vector instead of pCFD4. flySAM2.0 induces higher levels
of gene overexpression and only requires a single gRNA design. flySAM2.0 also includes a UAS-dCas9 activator so
that any Gal4 line can be crossed to a TRiP-OE line to drive gene overexpression.