HRMA - High Resolution Melt Analysis

Housden BE, Perrimon N. Cas9-based genome editing in Drosophila (Methods in Enzymology)

To begin, please upload your data file (or you may use our sample file to try things out) and indicate the quality control cutoff. Figure 1 will be generated for you and you will be prompted to set the remaining parameters. Click here for input file format requirements.

Set normalization ranges by dragging colored lines or entering temperature limits into the boxes. Turn on overlay to consider only curve shape and not position.

Select parameters for data analysis.

Input data file:

Data quality cutoff:
Input lower normalization range start:

Input lower normalization range end:

Input higher normalization range start:

Input higher normalization range end:


Overlay position (0-1):
P-value cutoff:

Sensitivity (0-1):

Absolute clustering?

Number of clusters to return:

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