Protein Alignment SCN8A and scn8aa

DIOPT Version :8

• Sequence 1: NP_001317189.1
• Gene: SCN8A / 6334
• HGNCID: 10596
• Length: 1980
• Species: Homo sapiens
• Sequence 2: XP_005162325.1
• Gene: scn8aa / 58152
• ZFINID: ZDB-GENE-000828-1
• Length: 1960
• Species: Danio rerio
• Alignment Length: 1994
• Identity: 1632/1995 (82%)
• Similarity: 1784/1995 (89%)
• Gaps: 50/1995 (3%)

Human     1 MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHREDDEDSKPKPNSDLEAGKSL 65
            |||.|||||||:|:|.||.|||..||:||.|.|.|.|||.|.|:|:||:::|||||.||||||||
Zfish     1 MAAPLLAPPGPNSYKYFTRESLREIEKRIEEEKAKPPPKPDNSYRDDDDENKPKPNGDLEAGKSL 65

Human    66 PFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRIAIKILI 130
            ||||||||.|:||.|||||||:|:.||||||||:|||:||||||||||::|||||.|||||||||
Zfish    66 PFIYGDIPPGMVATPLEDFDPFYINQKTFVVLNKGKTIFRFSATPALYMISPFNLARRIAIKILI 130

Human   131 HSVFSMIIMCTILTNCVFMTFSNPPDWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWN 195
            ||||||.||||||||||||||||||:|||.|||||||||||||.|||||||||||||||||||||
Zfish   131 HSVFSMFIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESAVKIIARGFCIDGFTFLRDPWN 195

Human   196 WLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVF 260
            ||||.||.|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish   196 WLDFMVISMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVF 260

Human   261 CLSVFALIGLQLFMGNLRNKCVVWPINFNESYLE-NGTKGFDWEEYINNKTNFYTVPGMLEPLLC 324
            ||||||||||||||||||.|||:||||..|.:.: ||::.|:|:|||.|:||||.:|..|:.|||
Zfish   261 CLSVFALIGLQLFMGNLRQKCVIWPINITEMFPDVNGSRAFNWDEYILNETNFYFLPDQLDALLC 325

Human   325 GNSSDAGQCPEGYQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMI 389
            |||||:|:|||||.||||||||||||||||:|.|||||||||||||:||||||||||||||||||
Zfish   326 GNSSDSGRCPEGYTCMKAGRNPNYGYTSFDSFGWAFLALFRLMTQDFWENLYQLTLRAAGKTYMI 390

Human   390 FFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATS 454
            ||||||||||||||||||||||||||||||||:||||:|||||||||||||||||:|||.|||||
Zfish   391 FFVLVIFVGSFYLVNLILAVVAMAYEEQNQATMEEAERKEAEFKAMLEQLKKQQEDAQANAMATS 455

Human   455 AGTVSEDAIEEEGEEGGGSPRSSSEISKLSSKSAKERRNRRKKRKQKELSEGEEKGDPEKVFKSE 519
            |||||||.:|::|:..|....||||:||||||||||||||:||.:|||  :.:||||.||..|||
Zfish   456 AGTVSEDVVEDDGDGEGNLSCSSSEMSKLSSKSAKERRNRKKKWRQKE--QDKEKGDSEKFVKSE 518

Human   520 SEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIFSFRGPGRFRDPGSENEFA 584
            |:||.||: ||.||||:||:.|||||||||||||||.||.||||||||     |.:|.|||||||
Zfish   519 SDDGSRRR-FRFPDNRLGRRSSIMNQSLLSIPGSPFPSRRNSKSSIFS-----RCKDGGSENEFA 577

Human   585 DDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQGSRSSRIFPSLRRSVKRNSTVDCNGVV 649
            |||||||||.:.||||...|    :|||||:|:.|                  ||||||||||||
Zfish   578 DDEHSTVEEYDERRDSFLSP----QRRSSYTGFYG------------------KRNSTVDCNGVV 620

Human   650 SLIGGPGSHIGGRLLPEA-----------TTEVEIKKKGPGSLLVSMDQL-ASYGRKDRINSIMS 702
            ||| |||.  |||||||.           ||::|||||..|||:||::|| .|:|||:|.||:||
Zfish   621 SLI-GPGP--GGRLLPEVIIDKAATDDSPTTDLEIKKKLSGSLMVSVEQLNTSFGRKERANSVMS 682

Human   703 VVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVDLAITICIVLNT 767
            .:|||||||||||||||||||||||||||||||.|.|:.:|||||||||||||||||||||||||
Zfish   683 ALTNTLVEELEESQRKCPPCWYKFANTFLIWECGPLWMSIKEIVNLIVMDPFVDLAITICIVLNT 747

Human   768 LFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMEL 832
            |||||||:||||||||||:|||||||||||||||.||:|||||||||||||||||||||||||||
Zfish   748 LFMAMEHYPMTPQFEHVLSVGNLVFTGIFTAEMFAKLVAMDPYYYFQEGWNIFDGFIVSLSLMEL 812

Human   833 SLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLF 897
            .||:|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish   813 GLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLF 877

Human   898 GKSYKECVCKINQDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMV 962
            |||||:|||||.||||||||||:||||||||||||||||||||||||||||||||||||||||||
Zfish   878 GKSYKDCVCKIAQDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMV 942

Human   963 IGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWTKLKVHAFMQAHFKQRE 1027
            |||||||||||||||||||||||||:|||||||||||:|||||||:||.|.||...:.....::.
Zfish   943 IGNLVVLNLFLALLLSSFSADNLAASDDDGEMNNLQIAVIRIKKGIAWVKAKVRELVNIILGRKV 1007

Human  1028 ADEVKPLDELYEKKANCIANHTGADIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNPNL 1092
            .||.||||::|::|.||||||||.||.|:.|:|||||||||||||||.||:||:||||||:||||
Zfish  1008 TDEAKPLDDMYDRKLNCIANHTGVDISRDLDYQKNGNGTTSGIGSSVGKYMIDDDHMSFIHNPNL 1072

Human  1093 TVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEGSTIDIKPEVEE-VPVEQPEEYLD 1156
            ||.|||||||||||||||||.||||:.||||: |||.|||||||||||||||| |.||..|||:|
Zfish  1073 TVCVPIAVGESDFENLNTEDFSSESEAEGSKE-LDDISSSEGSTIDIKPEVEEAVVVETVEEYVD 1136

Human  1157 PDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMILLSSGALAFEDIY 1221
            |:||:||.|:.|:|||.|.|.||.||:||.|||||:||||||||||.||||||||||||||||:|
Zfish  1137 PEACWTEACIARYKCCDVPITEGWGKNWWFLRKTCYLIVEHNWFETLIIFMILLSSGALAFEDVY 1201

Human  1222 IEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLIVAVSLVSLIANALGY 1286
            ||||||||.||||||.||||||||||||||.||||||:||||||||||.||.||:||||||||||
Zfish  1202 IEQRKTIRIILEYADMVFTYIFILEMLLKWVAYGFVKYFTNAWCWLDFFIVDVSIVSLIANALGY 1266

Human  1287 SELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAG 1351
            |:||.||||||||||||||||||||||||||||||||||||||||||||||||||||||||:|||
Zfish  1267 SDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFAG 1331

Human  1352 KYHYCFNETSEIRFEIEDVNNKTECEKLMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWM 1416
            ||:||:|||.:..||::.||||:||..|:|.|.||:|||||||||||||||||||||||||||||
Zfish  1332 KYYYCYNETEKAYFELDVVNNKSECFALIEQNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWM 1396

Human  1417 DIMYAAVDSRKPDEQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFM 1481
            ||||||||||:.::|||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish  1397 DIMYAAVDSRRVEDQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFM 1461

Human  1482 TEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQ 1546
            ||||||||||||||||||||||||||.||:||:||||||||.|||.||:||||||||||||||.|
Zfish  1462 TEEQKKYYNAMKKLGSKKPQKPIPRPQNKLQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQ 1526

Human  1547 SKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFV 1611
            |::.||||||||.:|::.||.|.|||:|||||||||.||||||.||||||||||||||:||||||
Zfish  1527 SQETENILYWINFIFIVAFTSEFVLKLFALRHYYFTNGWNIFDCVVVILSIVGMFLADLIEKYFV 1591

Human  1612 SPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYV 1676
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish  1592 SPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYV 1656

Human  1677 KHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSV 1741
            |.|:|||||:|||||||||||||.|||||||||||.||||.||||...||:||:..||:||||||
Zfish  1657 KRESGIDDMYNFETFGNSMICLFMITTSAGWDGLLAPILNYPPDCEPTKENPGTSVKGNCGNPSV 1721

Human  1742 GIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYC 1806
            ||||||.|||:||||||||||||||||||||||||||||.|||||:||||||||||.|:|||.:.
Zfish  1722 GIFFFVMYIIVSFLIVVNMYIAIILENFSVATEESADPLCEDDFESFYEIWEKFDPTASQFITFA 1786

Human  1807 KLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELDILRQQMEE 1871
            ||.||||.||||||||||||||||||||||||||||||||||||||||||||||:||::||||||
Zfish  1787 KLGDFADTLEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGDLDMMRQQMEE 1851

Human  1872 RFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFICKK-TTSNKLENGGTHREKK 1935
            ||:|:||||||:||||||||||||.:||.|:|||:|.||.|:|||||: .:|::||||||:::||
Zfish  1852 RFIAANPSKVSFEPITTTLRRKQEHMSAGVIQRAFRAHLIRKGFICKRLLSSSRLENGGTNQDKK 1916

Human  1936 ESTPSTASLPSYDSVTKPEKEKQQRAEEGRRERAKRQKEVRESK 1979
            |.||||||||||||||||||||.:.: :.:.::.|.||:|:|||
Zfish  1917 EGTPSTASLPSYDSVTKPEKEKLEES-DSKGKKGKNQKDVKESK 1959

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SCN8A	NP_001317189.1	I. {ECO:0000305}	114..442		290/329 (88%)
		Ion_trans	131..421	CDD:306908	257/291 (88%)
		Na_trans_cytopl	502..688	CDD:314757	116/197 (59%)
		II. {ECO:0000305}	735..1007		256/272 (94%)
		Ion_trans	752..965	CDD:306908	204/213 (96%)
		Na_trans_assoc	991..1192	CDD:310839	146/202 (72%)
		III. {ECO:0000305}	1180..1495		279/315 (89%)
		Ion_trans	1197..1473	CDD:306908	244/276 (88%)
		Na_channel_gate	1465..1517	CDD:240441	49/52 (94%)
		IV. {ECO:0000305}	1504..1801		256/297 (86%)
		Ion_trans	1522..1776	CDD:306908	220/254 (87%)
		GPHH	1780..1843	CDD:318993	54/63 (86%)
		IQ	1894..1915	CDD:197470	13/21 (62%)
scn8aa	XP_005162325.1	Ion_trans	151..423	CDD:278921	239/272 (88%)
		ATP-synt_B	363..>456	CDD:304375	88/93 (95%)
		Na_trans_cytopl	498..604	CDD:288761	79/118 (67%)
		Ion_trans	750..945	CDD:278921	186/195 (95%)
		Na_trans_assoc	971..1172	CDD:284034	146/202 (72%)
		Ion_trans	1195..1453	CDD:278921	227/258 (88%)
		Na_channel_gate	1445..1497	CDD:240441	49/52 (94%)
		Ion_trans	1519..1756	CDD:278921	206/237 (87%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930				C368792054
eggNOG	1	0.900				E1_KOG2301
Hieranoid	1	1.000
Homologene	1	1.000				H7927
Inparanoid	1	1.050	3239	1.000	Inparanoid score	I117
OMA	1	1.010
OrthoDB	1	1.010				D13170at7742
OrthoFinder	1	1.000				FOG0000322
OrthoInspector	1	1.000				T773205
orthoMCL	1	0.900				OOG5_126819
Panther	1	1.100			LDO	PTHR10037
Phylome	1	0.910		0.667	Consistency score
RoundUp	0	0.000	Not matched by this tool.
TreeFam	1	0.960
ZFIN	1	1.500
	14	14.270