Protein Alignment SCN8A and scn3a

DIOPT Version :10

• Sequence 1: NP_055006.1
• Gene: SCN8A / 6334
• HGNCID: 10596
• Length: 1980
• Species: Homo sapiens
• Sequence 2: XP_012826714.1
• Gene: scn3a / 100125099
• XenbaseID: XB-GENE-5812330
• Length: 2019
• Species: Xenopus tropicalis
• Alignment Length: 2036
• Identity: 1505/2036 - (73%)
• Similarity: 1691/2036 - (83%)
• Gaps: 75/2036 - (3%)

Human     1 MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHREDDEDSKPKPNSDLEAGKSL 65
            |...||.||||||.:..|.||||.:|:|.||.|.:|..:.|  .|:||:::.|||||||||||:|
 Frog     1 MEQPLLPPPGPDSLRLLTRESLAVLEKRFAEEKARKASQKD--RRDDDDENGPKPNSDLEAGKNL 63

Human    66 PFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRIAIKILI 130
            ||||||||||:||||:||.||||..||||:||||||.:||||||.|||||:|||.:|||:||||:
 Frog    64 PFIYGDIPQGMVAVPMEDLDPYYSNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRRISIKILV 128

Human   131 HSVFSMIIMCTILTNCVFMTFSNPPDWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWN 195
            ||:|||:|||||||||.|||.||||:|:|||||||||||||||||||:|||||::.|||||||||
 Frog   129 HSLFSMLIMCTILTNCFFMTMSNPPEWTKNVEYTFTGIYTFESLVKILARGFCLESFTFLRDPWN 193

Human   196 WLDFSVIMMAYITEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVF 260
            |||||||:|||:||||:||||||||||||||||||||||||||||||||||||||||||||||||
 Frog   194 WLDFSVIVMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVF 258

Human   261 CLSVFALIGLQLFMGNLRNKCVVWPIN--------FNES---YLENGTKGFDWEEYINNKTNFYT 314
            |||||||||||||||:||||||.||..        |||:   |:......|:.||||..:||||.
 Frog   259 CLSVFALIGLQLFMGHLRNKCVRWPPEYEPNITSYFNETAGLYINVTLNPFNREEYILEETNFYF 323

Human   315 VPGMLEPLLCGNSSDAGQCPEGYQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLT 379
            :||..:.|||||||||||||:||.|:|.|||||||||||||||||||:||||||||:||||||||
 Frog   324 LPGTRDALLCGNSSDAGQCPKGYVCLKTGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLT 388

Human   380 LRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQE 444
            |||||||||||||||||:|||||:|||||||||||||||||||||||||||||:.|:|||||.||
 Frog   389 LRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQLMMEQLKKHQE 453

Human   445 EAQAAAMATSAGTVSEDAIEEEGEEG-GGSPRSSSEISKLSSKSAKERRNRRKKRKQKELSEGEE 508
            |.||||:|..   ..:.:.|..|..| ||...||||.||.|||||||||||||||||||..| :|
 Frog   454 EIQAAALAAE---TMDGSREFSGGTGYGGLSESSSEASKPSSKSAKERRNRRKKRKQKEQGE-DE 514

Human   509 KGDPEKVFKSESEDGMRRKAFR--LPDNRI--GRKFSIMNQSLLSIPGSPFLSRHNSKSSIFSFR 569
            |.|.||..||.||..::||.||  :..||:  .:|:|..:||.||..||.|..|.:|::|:.|||
 Frog   515 KADDEKFHKSASETSIKRKGFRFSIEGNRLTYEKKYSSPHQSRLSFRGSLFFPRRDSRASLCSFR 579

Human   570 GPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQGSRSSRIFPSLR 634
            |.|  :|.||||:||||||||.|::|.||.|||:|.|..|||:     |..||.|::||..|.|.
 Frog   580 GRG--KDVGSENDFADDEHSTFEDNESRRGSLFVPHRHGERRN-----SNISQTSKASRTLPVLP 637

Human   635 RSVKRNSTVDCNGVVSLIGGPG--SHIGGRLLPE------------ATTEVEIKKKGPGSLLVSM 685
            .:.|.:|||||||||||:|||.  :...|.||||            ..||.|::|:...|..|||
 Frog   638 VNGKMHSTVDCNGVVSLLGGPSGPTSPAGLLLPEVIIDKPASDDNGTNTENELRKRRLSSYHVSM 702

Human   686 DQLASYGRKDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIV 750
            |.|.....:.|..||.|.:|||: ||||||:.||||||.|||||:|||:|...|:|:|.:|||||
 Frog   703 DFLEDPILRQRALSIASNLTNTM-EELEESREKCPPCWNKFANTYLIWDCSDSWLKIKHVVNLIV 766

Human   751 MDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQE 815
            ||||||||||||||||||||||||:|:|.:|...|.||||||||||||||..||||:|||||||.
 Frog   767 MDPFVDLAITICIVLNTLFMAMEHYPITDEFAGALNVGNLVFTGIFTAEMVFKLIALDPYYYFQV 831

Human   816 GWNIFDGFIVSLSLMELSLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTL 880
            |||||||.|||||||||.|:|||||||||||||||||||||||||||||||||||||||||||||
 Frog   832 GWNIFDGVIVSLSLMELGLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTL 896

Human   881 VLAIIVFIFAVVGMQLFGKSYKECVCKI-NQDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDC 944
            |||||||||||||||||||||||||||| ..|||||||||:||||||||||||||||||||||||
 Frog   897 VLAIIVFIFAVVGMQLFGKSYKECVCKIAPDDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDC 961

Human   945 MEVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVA 1009
            ||||||||||.||||||||||||||||||||||||||:||||||:||.|||||||:|.|:.:|:|
 Frog   962 MEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATEDDNEMNNLQIAVARMHEGIA 1026

Human  1010 WTKLKVHAFMQAHF--KQREADEVKPLDELYEKKANCIANHTGADIHRNGDFQKNGNGT--TSGI 1070
            :.|.|:..|:|..|  ||:..:|.|||:::..:|.:||:|||..:|.:..|:.|:||.|  ||||
 Frog  1027 YVKRKMREFIQNAFVKKQKALEETKPLEDMQNQKDSCISNHTTVEISKEYDYLKDGNVTTATSGI 1091

Human  1071 GSSVEKYIIDE-DHMSFINNPNLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEG 1134
            ||||||||||| |:||||:||:|||.||||||||||||||||:.|||||.|.||:||:.:|||||
 Frog  1092 GSSVEKYIIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEG 1156

Human  1135 STIDIKP--EVEEVPVEQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEH 1197
            ||:||:|  |.|.||..:.||:.:||||||:||::||.||.|::..|..|.||.|||||||||||
 Frog  1157 STVDIRPAGEGEPVPQVEVEEFQEPDACFTDGCIRRFPCCVVDVSHGKWKQWWNLRKTCFLIVEH 1221

Human  1198 NWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTN 1262
            ||||||||||||:||||||||||||||||||:.|||||||||||||||||||||.||||.|:|||
 Frog  1222 NWFETFIIFMILMSSGALAFEDIYIEQRKTIKIILEYADKVFTYIFILEMLLKWVAYGFQKYFTN 1286

Human  1263 AWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSI 1327
            ||||||||||.||||||||||||||||||||||||||||||||||||||||||||||||||||||
 Frog  1287 AWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSI 1351

Human  1328 MNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIEDVNNKTECEKLMEGNNTEIRWKNV 1392
            ||||||||||||||||||||:|:|||.||.|.|.::.|:...|||:::|..|:..|.| .|||||
 Frog  1352 MNVLLVCLIFWLIFSIMGVNMFSGKYGYCVNYTDDVVFDYTIVNNRSQCMDLISENKT-ARWKNV 1415

Human  1393 KINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPKYEDNIYMYIYFVIFIIFGSFFTLN 1457
            |:||||||.|||||||||||||||:||||||||||.:||||||||:|||:|||||||||||||||
 Frog  1416 KVNFDNVGFGYLALLQVATFKGWMEIMYAAVDSRKQEEQPKYEDNLYMYLYFVIFIIFGSFFTLN 1480

Human  1458 LFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQ 1522
            ||||||||||||||||||||||||||||||||||||||||||||||:|||.||.||.|||||::|
 Frog  1481 LFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPVPRPDNKFQGAVFDFVSKQ 1545

Human  1523 AFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNI 1587
            .|||.||:||||||||||:|||.||.:.||.|||||.||::.||.|||||:.:||||||||||||
 Frog  1546 PFDIAIMILICLNMVTMMIETDDQSIETENNLYWINAVFIVLFTGECVLKLISLRHYYFTIGWNI 1610

Human  1588 FDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALF 1652
            |||||||||||||||||:|||||||||||||||||||||||||||||||||||||||||||||||
 Frog  1611 FDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALF 1675

Human  1653 NIGLLLFLVMFIFSIFGMSNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNR 1717
            ||||||||||||::|||||||||||.|||||||||||||||||||||||||||||||||.||||.
 Frog  1676 NIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNS 1740

Human  1718 -PPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLS 1781
             .|||....||.||..|||||||||||||||||||||||:|||||||:|||||||||||||:||.
 Frog  1741 GAPDCDPKAEHSGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLG 1805

Human  1782 EDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLD 1846
            |||||.|||:|||||.:|||||:|.||.||||||:.|||:||||..:||||||||||||||||||
 Frog  1806 EDDFEMFYEVWEKFDSNATQFIQYVKLTDFADALDPPLRMPKPNNAQLIAMDLPMVSGDRIHCLD 1870

Human  1847 ILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLA 1911
            :||||||||||:..|:|.|||.|||||:||||||.||||||:||||||||::|:|:||.||.:..
 Frog  1871 VLFAFTKRVLGEGDEMDNLRQPMEERFMASNPSKASYEPITSTLRRKQEELAAIVIQRCYRCYAL 1935

Human  1912 RRG------------------FICKKTTSNKLENGGTHREKKESTPSTASLPSYDSVTKPEKEKQ 1958
            |:|                  .:.:|...:...||.:..||.:.||||.|.|||||||||:|||.
 Frog  1936 RKGIKHASFMYNQEKGKDGGSLLTRKDMLSDRLNGNSTTEKADITPSTTSPPSYDSVTKPDKEKH 2000

Human  1959 QRAEEGRRERAKRQKEVRESK 1979
               |:.:.|:..|.|:|||.|
 Frog  2001 ---EKDKIEKEDRGKDVREHK 2018

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SCN8A	NP_055006.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..20		10/18 (56%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	28..62		17/33 (52%)
		I. /evidence=ECO:0000305	114..442		275/338 (81%)
		Ion_trans	131..421	CDD:459842	245/300 (82%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	446..530		47/84 (56%)
		Na_trans_cytopl	546..702	CDD:463401	80/169 (47%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	568..602		22/33 (67%)
		II. /evidence=ECO:0000305	735..1007		238/272 (88%)
		Ion_trans	752..965	CDD:459842	193/213 (91%)
		Na_trans_assoc	991..1192	CDD:461936	123/207 (59%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1107..1148		28/42 (67%)
		III. /evidence=ECO:0000305	1180..1495		272/314 (87%)
		Ion_trans	1197..1473	CDD:459842	237/275 (86%)
		Na_channel_gate	1465..1517	CDD:240441	47/51 (92%)
		IV. /evidence=ECO:0000305	1504..1801		251/297 (85%)
		Ion_trans	1522..1776	CDD:459842	220/254 (87%)
		IQ	1894..1915	CDD:197470	11/38 (29%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1922..1980		29/58 (50%)
scn3a	XP_012826714.1	Ion_trans	129..430	CDD:459842	245/300 (82%)
		Na_trans_cytopl	556..719	CDD:463401	80/169 (47%)
		Ion_trans	768..982	CDD:459842	193/213 (91%)
		Na_trans_assoc	1008..1216	CDD:461936	123/207 (59%)
		Ion_trans	1221..1496	CDD:459842	237/275 (86%)
		Na_channel_gate	1488..1540	CDD:240441	47/51 (92%)
		Ion_trans	1545..1800	CDD:459842	220/254 (87%)
		IQCD	1915..1952	CDD:467745	14/36 (39%)