Protein Alignment SCN1A and scn1lab

DIOPT Version :8

• Sequence 1: NP_001159435.1
• Gene: SCN1A / 6323
• HGNCID: 10585
• Length: 2009
• Species: Homo sapiens
• Sequence 2: NP_001038360.2
• Gene: scn1lab / 559447
• ZFINID: ZDB-GENE-060906-1
• Length: 1996
• Species: Danio rerio
• Alignment Length: 2023
• Identity: 1576/2024 (78%)
• Similarity: 1769/2024 (87%)
• Gaps: 48/2024 (2%)

Human     1 MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKK--DDDENGPKPNSDLEAGKNLPF 63
            |...:|||||||||..|..|||||||:|||||.|:.||.:::  ||||||||||.||||||.|||
Zfish     1 MAAQLLVPPGPDSFRPFLPESLAAIEQRIAEEVAQRPKGERRPDDDDENGPKPNRDLEAGKPLPF 65

Human    64 IYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHS 128
            ||||:||.|:|.||||||.||.|:|||||||:|||||||:||.|||||:||||||:|:|::||||
Zfish    66 IYGDVPPHMLSVPLEDLDTYYSNQKTFIVLNRGKAIFRFNATPALYILSPFNPLRRISIRVLVHS 130

Human   129 LFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWL 193
            |||.:|||||||||.|||:||||:|.|||||||||||||||||||:|||||:..|||||||||||
Zfish   131 LFSFVIMCTILTNCAFMTLSNPPEWAKNVEYTFTGIYTFESLIKILARGFCVGKFTFLRDPWNWL 195

Human   194 DFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCL 258
            ||:||..|||||||:||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish   196 DFSVILMAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCL 260

Human   259 SVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRY 323
            ||||||||||||||||.||::.|..|       :..|:| :|:..|.|.|   |:|..||.||..
Zfish   261 SVFALIGLQLFMGNLRQKCVKMPEVN-------VTDNLT-DYSTELHNST---FNWTEYISDSNN 314

Human   324 HYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLY 388
            :||:.|..|||||||||||||||||:.|:||||||:|||||||||||||||||||||||||||||
Zfish   315 YYFIPGRRDALLCGNSSDAGQCPEGFTCIKAGRNPDYGYTSFDTFSWAFLSLFRLMTQDFWENLY 379

Human   389 QLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKK 453
            |.|||||||.||||||||||||||||:|||||||||||:||||||:|||:|||.|||.|:||||:
Zfish   380 QQTLRAAGKPYMIFFVLVIFLGSFYLVNLILAVVAMAYDEQNQATIEEAQQKEEEFQAMLEQLKR 444

Human   454 QQEAAQQAATATASEHSREPSAAGRLS-DSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDE 517
            |||.||.|| |.|:| |.|.|..|.|| :|||..|:||||||||||||||||||:|:.  |:.|:
Zfish   445 QQEEAQVAA-AAATE-SGEYSGRGGLSEESSSGGSRLSSKSAKERRNRRKKRKQREEE--EKADQ 505

Human   518 DEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAK 582
            ::|.||.|||||||.||||||:.|||:|||:.|:|:||||||||||||||||||.|||||||||:
Zfish   506 EKFHKSASEDSIRRPGFRFSIDANRLSYEKKCSTPNQSLLSIRGSLFSPRRNSRASLFSFRGRAR 570

Human   583 DVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDC 647
            |.||||||||||||||||::|||.||||| |..|||:|.:|..|.::..: :.|||||||.||||
Zfish   571 DFGSENDFADDEHSTFEDSDSRRGSLFVP-RCVERRSSTVSHCSLTAPRI-MLPANGKMHCTVDC 633

Human   648 NGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQR 712
            |||||||||.||||||:|:||||           |||||:::||:||.| |...|:||:...|:|
Zfish   634 NGVVSLVGGTSVPTSPIGRLLPE-----------GTTTESDVRKKRSGS-HQPSDYLEETVARKR 686

Human   713 AMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITIC 777
            |||:|||||||:|||||||||||||||||:|.||||||||.|||||.::|::|||||||||||||
Zfish   687 AMSVASILTNTMEELEESRQKCPPCWYKFANSFLIWDCSPAWLKVKEIMNMIVMDPFVDLAITIC 751

Human   778 IVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTL 842
            ||||||||||||||||..|:::|:||||||||||||||.||:||:|||||||||||||||.||:|
Zfish   752 IVLNTLFMAMEHYPMTTQFSHLLSVGNLVFTGIFTAEMCLKVIALDPYYYFQEGWNIFDGIIVSL 816

Human   843 SLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVV 907
            ||:||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish   817 SLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVV 881

Human   908 GMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVF 972
            |||||||||::|||||:..|:||||||:||||||||||||||||||||||||||||||:|||.||
Zfish   882 GMQLFGKSYRECVCKISESCELPRWHMDDFFHSFLIVFRVLCGEWIETMWDCMEVAGQSMCLIVF 946

Human   973 MMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQS 1037
            ||||||||||||||||||||||||||||||||||:|||||||||.|:.||:|.||..:.:|:|..
Zfish   947 MMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDSEMNNLQIAVGRIQKGIALVKSTLRQFLQSL 1011

Human  1038 FI--RKQKILDEIKPLDDLNNK-KDSCMSNHTAEIGKDL--DYLKDVNGTTS-GIGTGSSVEKYI 1096
            |:  .|.:.|||.||||||::. |::|:||| |.:..||  ||||:  |..| |.|..|.| ||.
Zfish  1012 FLAGSKPRSLDEEKPLDDLHSDGKENCLSNH-APVAVDLTKDYLKE--GCVSPGNGVESHV-KYG 1072

Human  1097 IDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSE-SDLEESKEKLNESS--SSSEGSTVD 1158
            |:|.||||||:|||||||||||||||||||||||||||| ||:|.|||||...:  |||||||||
Zfish  1073 IEEGDYMSFIHNPSLTVTVPIAVGESDFENLNTEDFSSESSDVEGSKEKLPVDTVLSSSEGSTVD 1137

Human  1159 IGAPVE--EQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFE 1221
            |..|.|  |...:||||:|:||||||||||.||:|||::||:|:.|.||.||:|||.||||||||
Zfish  1138 IRPPGEGAESVELEPEESLDPEACFTEGCVMRFQCCQVDVEKGKWKSWWILRKTCFIIVEHNWFE 1202

Human  1222 TFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCW 1286
            :||:|||||||||||||||||:|||||||:||||||||||||||||||||||||:..||||||||
Zfish  1203 SFIIFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYIFILEMLLKWVAYGFAKYFTNAWCW 1267

Human  1287 LDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVL 1351
            |||||||||||||.|||||||||.|||||||||||||||||||||||||||||||||||||||||
Zfish  1268 LDFLIVDVSLVSLVANALGYSELSAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVL 1332

Human  1352 LVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFD 1416
            |||||||||||||||||||||:|:|:|.|..:.|.:..|||.:||..||:.|:||||||||:|||
Zfish  1333 LVCLIFWLIFSIMGVNLFAGKYYYCVNITNDELFPVSVVNNKSDCYALIQNNDTARWKNVKINFD 1397

Human  1417 NVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGV 1481
            |||.|||:|||||||||||||||||||||::|.||.||.:|||||||||||||||||||||||||
Zfish  1398 NVGAGYLALLQVATFKGWMDIMYAAVDSRDLEQQPVYETNLYMYLYFVIFIIFGSFFTLNLFIGV 1462

Human  1482 IIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDIS 1546
            |||||||||||.|||||||||||||||||||||||||||||||||.|||||:|||.:|:|.|||.
Zfish  1463 IIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGLVFDLITKQAFDII 1527

Human  1547 IMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVV 1611
            |||||||||||||||||||:|.:..||..||||||:|||||||||:||||||:||||||:|||||
Zfish  1528 IMILICLNMVTMMVETDDQTEDMDNILYWINLVFIILFTGECVLKMISLRHYFFTIGWNVFDFVV 1592

Human  1612 VILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLL 1676
            ||||||||||:|:||||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish  1593 VILSIVGMFLSEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLL 1657

Human  1677 LFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDC 1741
            ||||||||||||||||||||||.|||||||||||||||:||||||||||||||||||||.:.|||
Zfish  1658 LFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMLCLFQITTSAGWDGLLAPILNKQDPDC 1722

Human  1742 DPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFE 1806
            |....:|||..:|:|||||||||||||||||.||:||||||||||||||||||||||||||||||
Zfish  1723 DSEMEHPGSFYRGNCGNPSVGIFFFVSYIIICFLIVVNMYIAVILENFSVATEESAEPLSEDDFE 1787

Human  1807 MFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAF 1871
            |||||||:|||:||||:|:.|||.||.||:|||.:.:|||:||||||||||||||||||||||||
Zfish  1788 MFYEVWERFDPNATQFVEYNKLSDFADALDPPLRIAKPNKIQLIAMDLPMVSGDRIHCLDILFAF 1852

Human  1872 TKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVK 1936
            |.|||||.|||||||.|||:||||||||||||:||||||:|||||:|||:||||:|||.:::|||
Zfish  1853 TLRVLGEEGEMDALRGQMEDRFMASNPSKVSYEPITTTLRRKQEEMSAVVIQRAFRRHRIRQTVK 1917

Human  1937 QASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEG 2001
            :||..|.|....||.::..||.::|.:..|||.::|||:|.|:|: ||||:.|.|...:|:|:|.
Zfish  1918 KASEAYRKQLQDGGGHIPEKEVLVIGKFQENSASDKTDMTPSSAS-PPSYNSVAKSEKDKYEKER 1981

Human  2002 KDEKAKGK 2009
            ::::.|.|
Zfish  1982 REKEDKAK 1989

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SCN1A	NP_001159435.1	I. {ECO:0000305}	110..454		273/344 (79%)
		Ion_trans	127..433	CDD:306908	246/306 (80%)
		Na_trans_cytopl	510..705	CDD:314757	129/195 (66%)
		II. {ECO:0000305}	750..1022		241/272 (89%)
		Ion_trans	767..980	CDD:306908	192/213 (90%)
		Na_trans_assoc	1006..1213	CDD:310839	137/218 (63%)
		III. {ECO:0000305}	1200..1514		270/314 (86%)
		Ion_trans	1217..1492	CDD:306908	238/275 (87%)
		Na_channel_gate	1484..1536	CDD:240441	49/52 (94%)
		IV. {ECO:0000305}	1523..1821		260/298 (87%)
		Ion_trans	1540..1796	CDD:306908	224/256 (88%)
		S1-S2 loop of repeat IV. {ECO:0000250|UniProtKB:A2APX8}	1561..1571		6/10 (60%)
		S3b-S4 loop of repeat IV. {ECO:0000250|UniProtKB:A2APX8}	1619..1636		15/17 (88%)
		GPHH	1800..1863	CDD:318993	50/63 (79%)
scn1lab	NP_001038360.2	None

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
eggNOG	1	0.900				E1_KOG2301
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	3177	1.000	Inparanoid score	I126
OMA	1	1.010
OrthoDB	1	1.010				D13587at7742
OrthoFinder	1	1.000				FOG0000322
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900				OOG5_126819
Panther	1	1.100			O	PTHR10037
Phylome	1	0.910		0.909	Consistency score
RoundUp	1	1.030		avgDist	Average_Evolutionary_Distance	R9280
TreeFam	1	0.960
ZFIN	0	0.000	Not matched by this tool.
	10	9.870