Protein Alignment SCN1A and scn1a

DIOPT Version :8

• Sequence 1: NP_001159435.1
• Gene: SCN1A / 6323
• HGNCID: 10585
• Length: 2009
• Species: Homo sapiens
• Sequence 2: XP_012826731.1
• Gene: scn1a / 100488621
• XenbaseID: XB-GENE-978019
• Length: 2020
• Species: Xenopus tropicalis
• Alignment Length: 2028
• Identity: 1670/2029 (82%)
• Similarity: 1825/2029 (90%)
• Gaps: 34/2029 (2%)

Human     1 MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPD-KKDDDENGPKPNSDLEAGKNLPFI 64
            |.|..|||||||||.:|:||||.|||:||||||||.||.: :.|||:|||||:|||||||:||||
 Frog     1 MAQARLVPPGPDSFRYFSRESLEAIEKRIAEEKAKKPKQEHRDDDDDNGPKPSSDLEAGKSLPFI 65

Human    65 YGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSL 129
            ||||||.|||||||||||||:|:|||||||:||||||||||||||||||||||||:|||:|||||
 Frog    66 YGDIPPGMVSEPLEDLDPYYMNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRKVAIKVLVHSL 130

Human   130 FSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLD 194
            ||||||||||||||||||||||:|||||||||||||||||||||:|||||||.||||||||||||
 Frog   131 FSMLIMCTILTNCVFMTMSNPPEWTKNVEYTFTGIYTFESLIKILARGFCLEKFTFLRDPWNWLD 195

Human   195 FTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLS 259
            |:||..|||||||:|||||||||||||||||||||||||||||||||||||||||||||||||||
 Frog   196 FSVIVMAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLS 260

Human   260 VFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLI------NETVFEFDWKSYI 318
            ||||||||||||:|||||:||||.     ::....||....|||:.      |.|...|||:.||
 Frog   261 VFALIGLQLFMGHLRNKCLQWPPI-----DYFPLTNIPPILNGTMNIHDPFGNTTNSTFDWEEYI 320

Human   319 QDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDF 383
            .|..:.|.|||..|.||||||||:||||:||:|||.|:||||||||||||||||||||||||||:
 Frog   321 TDKNHFYKLEGQKDFLLCGNSSDSGQCPKGYICVKTGKNPNYGYTSFDTFSWAFLSLFRLMTQDY 385

Human   384 WENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMI 448
            |||||||||||||||||||||||||||||||:||||||||||||||||||||||||||||||||:
 Frog   386 WENLYQLTLRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFQQML 450

Human   449 EQLKKQQEAAQQAATAT---ASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQS 510
            ||||||||.||.||.|.   :.|:| |....|.||:||||||||||||||:||||:|||||||..
 Frog   451 EQLKKQQEEAQAAAVAALNMSREYS-EDGGFGPLSESSSEASKLSSKSAKDRRNRKKKRKQKELG 514

Human   511 GGEEKDEDE-FQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSL 574
            ...||.:|| |.|||||.||:||||||||||||||||.::||||||||||||||||||||||.||
 Frog   515 EEYEKGDDEKFPKSESETSIKRKGFRFSIEGNRLTYENKFSSPHQSLLSIRGSLFSPRRNSRASL 579

Human   575 FSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANG 639
            ||||.||||||||||||||||||||||||||.|||||.|||||||||:||.|:||:.:.|.||||
 Frog   580 FSFRNRAKDVGSENDFADDEHSTFEDNESRRGSLFVPNRHGERRNSNISQVSKSSKTMTVLPANG 644

Human   640 KMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFL 704
            ||||||||||||||:|||||||||||.||||||:||||:||||||||||:|||:||.:.|.||.|
 Frog   645 KMHSTVDCNGVVSLLGGPSVPTSPVGLLLPEVIVDKPASDDNGTTTETEIRKRKSSCYQVPMDLL 709

Human   705 EDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPF 769
            |||:.||||:|:|||||||||||||||||||||||||:|.||||||...||.|||:|.|:|||||
 Frog   710 EDPTARQRALSVASILTNTVEELEESRQKCPPCWYKFANTFLIWDCCENWLTVKHIVKLIVMDPF 774

Human   770 VDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNI 834
            ||||||||||||||||||||||||..||:||::|||||||||||||..|:||:||||||||||||
 Frog   775 VDLAITICIVLNTLFMAMEHYPMTKEFNDVLSIGNLVFTGIFTAEMVFKLIALDPYYYFQEGWNI 839

Human   835 FDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAI 899
            |||.||:|||:||||||||||||||||||||||||||||||||||||||||||||||||||||||
 Frog   840 FDGIIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAI 904

Human   900 IVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAG 964
            ||||||||||||||||||:||||||.:|:||||||||||||||||||||||||||||||||||||
 Frog   905 IVFIFAVVGMQLFGKSYKECVCKIAENCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAG 969

Human   965 QAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRK 1029
            |:|||.|||:||||||||||||||||||||||:|||.|||||||||||||||.|:.:|:.|||:.
 Frog   970 QSMCLLVFMLVMVIGNLVVLNLFLALLLSSFSSDNLTATDDDNEMNNLQIAVGRIQRGIDYVKKT 1034

Human  1030 IYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTA-EIGKDLDYLKDVNGTTSGIGTGSSVE 1093
            |.|.||::|::|||.|||||||::|:||::||:.||.| ||.|.|||.|..||||||:  |||||
 Frog  1035 IRECIQKAFVKKQKGLDEIKPLEELHNKRESCLFNHNAVEINKVLDYHKGDNGTTSGV--GSSVE 1097

Human  1094 KYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVD 1158
            ||||||:||||||:||||||||||||||||||||||||||||||||||||||| .:|||||||||
 Frog  1098 KYIIDENDYMSFIHNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLN-MTSSSEGSTVD 1161

Human  1159 IGAP-----VEEQPV-VEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEH 1217
            |..|     .|.:|: :|.||.|||:||||||||:||..|.:|:||.:||.||..|:||:.||||
 Frog  1162 IRPPGVVAEGEGEPIDIESEEALEPDACFTEGCVKRFPICYVNIEENKGKLWWTFRKTCYTIVEH 1226

Human  1218 NWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTN 1282
            |||||||:|||||||||||||||||:||||||||||||||||||||||||||||||||:..||||
 Frog  1227 NWFETFIIFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFVKYFTN 1291

Human  1283 AWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSI 1347
            |||||||||||||||||.||||||||||||||||||||||||||||||||||||||||:||||||
 Frog  1292 AWCWLDFLIVDVSLVSLVANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSI 1356

Human  1348 MNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVK 1412
            |||||||||||||||||||||||||:|||:|||||:......|||.:|||.|   ||:|||||||
 Frog  1357 MNVLLVCLIFWLIFSIMGVNLFAGKYYHCVNTTTGEMLLDTQVNNRSDCLHL---NESARWKNVK 1418

Human  1413 VNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNL 1477
            |||||||.|||:|||||||||||||||||||||.||.||:||::|||||||||||||||||||||
 Frog  1419 VNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVEEQPRYEDNLYMYLYFVIFIIFGSFFTLNL 1483

Human  1478 FIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQV 1542
            |||||||||||||||||||||||||||||||||||||||||||||||||.||:||:||||||:|.
 Frog  1484 FIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPPNKYQGLVFDFVTKQA 1548

Human  1543 FDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIF 1607
            |||.|||||||||||||:|||:||:.:...|..||||||:||||||:||:|||||||||||||||
 Frog  1549 FDIIIMILICLNMVTMMIETDEQSQDMENNLYWINLVFIILFTGECILKIISLRHYYFTIGWNIF 1613

Human  1608 DFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFN 1672
            |||||||||||||||::||||||||||||||||||||||||||||||||||||||||||||||||
 Frog  1614 DFVVVILSIVGMFLADMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFN 1678

Human  1673 IGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSK 1737
            ||||||||||||||||||||||||:|.|||||||||||||||||||||||||||||||||||||.
 Frog  1679 IGLLLFLVMFIYAIFGMSNFAYVKKESGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSG 1743

Human  1738 PPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSE 1802
            .|||||...:|||||||||||||||||||||||||||||||||||||||||||||||||||||.|
 Frog  1744 APDCDPQMEHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGE 1808

Human  1803 DDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDI 1867
            ||||||||||||||||||||:|:.|||.||.||:|||.:.:||::||||||||||||||||||||
 Frog  1809 DDFEMFYEVWEKFDPDATQFIEYSKLSNFADALDPPLRIAKPNRVQLIAMDLPMVSGDRIHCLDI 1873

Human  1868 LFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLK 1932
            |||||||||||..||||||:.||||||||||||.||:|||:||:|||||:|.|||||.:|::||:
 Frog  1874 LFAFTKRVLGEGDEMDALRLPMEERFMASNPSKASYEPITSTLRRKQEELSVVIIQRCFRKYLLR 1938

Human  1933 RTVKQASFTYNKNKIKGGANLL-IKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEK 1996
            :|:|:||:.|.|.|:  |.|.| |||||:||::|.||.|||||:|.||.: |||||.||||..||
 Frog  1939 KTIKKASYLYKKEKL--GVNSLPIKEDMVIDKLNGNSTTEKTDMTPSTTS-PPSYDSVTKPEKEK 2000

Human  1997 HEQEGKDEKAKGK 2009
            ||::..:::.|.|
 Frog  2001 HEKDKAEKENKSK 2013

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SCN1A	NP_001159435.1	I. {ECO:0000305}	110..454		292/350 (83%)
		Ion_trans	127..433	CDD:306908	257/312 (82%)
		Na_trans_cytopl	510..705	CDD:314757	160/196 (82%)
		II. {ECO:0000305}	750..1022		240/272 (88%)
		Ion_trans	767..980	CDD:306908	194/213 (91%)
		Na_trans_assoc	1006..1213	CDD:310839	148/214 (69%)
		III. {ECO:0000305}	1200..1514		277/314 (88%)
		Ion_trans	1217..1492	CDD:306908	245/275 (89%)
		Na_channel_gate	1484..1536	CDD:240441	49/52 (94%)
		IV. {ECO:0000305}	1523..1821		268/298 (90%)
		Ion_trans	1540..1796	CDD:306908	230/256 (90%)
		S1-S2 loop of repeat IV. {ECO:0000250|UniProtKB:A2APX8}	1561..1571		5/10 (50%)
		S3b-S4 loop of repeat IV. {ECO:0000250|UniProtKB:A2APX8}	1619..1636		15/17 (88%)
		GPHH	1800..1863	CDD:318993	50/63 (79%)
scn1a	XP_012826731.1	Ion_trans	150..435	CDD:278921	235/290 (81%)
		Na_trans_cytopl	512..631	CDD:288761	99/119 (83%)
		Ion_trans	790..985	CDD:278921	176/195 (90%)
		Na_trans_assoc	1011..1222	CDD:284034	148/214 (69%)
		Ion_trans	1244..1498	CDD:278921	228/257 (89%)
		Na_channel_gate	1490..1542	CDD:240441	49/52 (94%)
		Ion_trans	1564..1802	CDD:278921	215/238 (90%)
		GPHH	1814..1869	CDD:293510	43/55 (78%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
eggNOG	1	0.900				E1_KOG2301
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010
OrthoDB	1	1.010				D10241at32523
OrthoFinder	1	1.000				FOG0000322
OrthoInspector	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
TreeFam	1	0.960
	5	4.880