Protein Alignment Hsp70Aa and hspa8

DIOPT Version :10

• Sequence 1: NP_731651.1
• Gene: Hsp70Aa / 48581
• FlyBaseID: FBgn0013275
• Length: 642
• Species: Drosophila melanogaster
• Sequence 2: NP_001103873.1
• Gene: hspa8 / 573376
• ZFINID: ZDB-GENE-990415-92
• Length: 649
• Species: Danio rerio
• Alignment Length: 647
• Identity: 477/647 - (73%)
• Similarity: 550/647 - (85%)
• Gaps: 8/647 - (1%)

  Fly     2 PAIGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFD 66
            ||:|||||||||||||:|||||||||||||||||||||||||:||||||.|||||||||.|||||
Zfish     5 PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFD 69

  Fly    67 AKRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAY 131
            |||||||::||..:..|||||||.|::|..:||:.||||||||.|.||||||||||||||.||||
Zfish    70 AKRLIGRRFDDGVVQSDMKHWPFNVINDNSRPKVQVEYKGESKSFYPEEISSMVLTKMKEIAEAY 134

  Fly   132 LGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196
            ||:::::|||||||||||||||||||||.|:|||||||||||||||:||||||.:..||||||||
Zfish   135 LGKTVSNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFD 199

  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLADEFKRKYKKDLRSNPRALRRL 261
            |||||||||||||::| :|||:||||||||||||||||:|.|...|||||:|||:..|.||:|||
Zfish   200 LGGGTFDVSILTIEDG-IFEVKSTAGDTHLGGEDFDNRMVNHFITEFKRKHKKDISDNKRAVRRL 263

  Fly   262 RTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDK 326
            |||.|||||||||||:|:||||:|:||.||||.::|||||||.|||||.||.||||||.||||||
Zfish   264 RTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKMDK 328

  Fly   327 GQIHDIVLVGGSTRIPKVQSLLQDFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLL 391
            .||||||||||||||||:|.||||:|:||.||.||||||||||||||||||||||:|..:||:||
Zfish   329 AQIHDIVLVGGSTRIPKIQKLLQDYFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLL 393

  Fly   392 VDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYADNQPGVSIQVYEGERAMTKDNNALGT 456
            :||.||||||||||||||.||:||..||.|||:||:||:||||||.|||||||||||||||.||.
Zfish   394 LDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGK 458

  Fly   457 FDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAE 521
            |:|:|||||||||||||||||:|||||:||||.:.||||...|||.|||||||:.:|:|||.|||
Zfish   459 FELTGIPPAPRGVPQIEVTFDIDANGIMNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAE 523

  Fly   522 KYADEDEKHRQRITSRNALESYVFNVKQAVE-QAPAGKLDEADKNSVLDKCNDTIRWLDSNTTAE 585
            ||..||:..|.:::::|.||||.||:|..|| :...||:.:.||..:|||||:.|.|||.|.|||
Zfish   524 KYKAEDDVQRDKVSAKNGLESYAFNMKSTVEDEKLKGKISDEDKQKILDKCNEVIGWLDKNQTAE 588

  Fly   586 KEEFDHKLEELTRHCSPIMTKMHQQGAGAGAGG-----PGANCGQQAGGFGGYSGPTVEEVD 642
            :|||:|:.:||.:.|:||:||:: |.||...||     ||...|..|...||.||||:||||
Zfish   589 REEFEHQQKELEKVCNPIITKLY-QSAGGMPGGMPEGMPGGFPGAGAAPGGGSSGPTIEEVD 649

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hsp70Aa	NP_731651.1	PTZ00009	2..642	CDD:240227	475/645 (74%)
hspa8	NP_001103873.1	PTZ00009	1..614	CDD:240227	459/610 (75%)
		Nucleotide-binding domain (NBD). /evidence=ECO:0000250|UniProtKB:P11142	2..386		309/381 (81%)
		Substrate-binding domain (SBD). /evidence=ECO:0000250|UniProtKB:P11142	394..509		90/114 (79%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	614..649		16/34 (47%)