Protein Alignment fwd and PI4KA

DIOPT Version :10

• Sequence 1: NP_001356971.1
• Gene: fwd / 45374
• FlyBaseID: FBgn0004373
• Length: 1682
• Species: Drosophila melanogaster
• Sequence 2: NP_477352.3
• Gene: PI4KA / 5297
• HGNCID: 8983
• Length: 2102
• Species: Homo sapiens
• Alignment Length: 1798
• Identity: 356/1798 - (19%)
• Similarity: 587/1798 - (32%)
• Gaps: 675/1798 - (37%)

  Fly   396 MCSKIA------TTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQSISLAAAC 454
            :|.|::      |::..:.:.|       |.|:  .|...:|.:  ..:.||..|    |:..:.
Human   458 LCIKLSEKLQSKTSSKVIIAHL-------PLLI--CCLQGLGRL--CERFPVVVH----SVTPSL 507

  Fly   455 RSSAVVESCPTLRMRPKSSE--SVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKR-EDLTS 516
            |...|:.|...:::....|:  :||.|     .:.:|.|...|.|:.|..|...|.... |.|..
Human   508 RDFLVIPSPVLVKLYKYHSQYHTVAGN-----DIKISVTNEHSESTLNVMSGKKSQPSMYEQLRD 567

  Fly   517 LGSDDSGIIC-----GSESDQISL--------NRICHSHESLDSGEMDADADAEEE--------- 559
            :..|:   ||     |...|.:.:        ||:..|.||      |.||....:
Human   568 IAIDN---ICRCLKAGLTVDPVIVEAFLASLSNRLYISQES------DKDAHLIPDHTIRALGHI 623

  Fly   560 CVDLMDTTSIDEEYNIMQPDHLCLYPPQAAST----ELDCRTLRPSRKQKRQQQQVLERKNLPSQ 620
            .|.|.||..:.|....:.....| .||.....    :|.|..:..::...::...:.::.::.:.
Human   624 AVALRDTPKVMEPILQILQQKFC-QPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKAS 687

  Fly   621 NQIES--EDSVDAGVDARTRYRVMNMSQAAINVELGTASNEIDPDV------------------- 664
            :.:.|  :|..|.|      ||  :.|.|.||.....|:|..|..:
Human   688 SVVYSATKDYKDHG------YR--HCSLAVINALANIAANIQDEHLVDELLMNLLELFVQLGLEG 744

  Fly   665 ---NYRESHQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNA 726
               :.|.|.:.|...|.|    |..:.....|.:.......|...|:....|.||::|:
Human   745 KRASERASEKGPALKASS----SAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFW------ 799

  Fly   727 IFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSV-PQDLVKSPTDISKKKEFFSLLT 790
                ..|:                       |:|....||.: |::..:...:|:.|.   .|||
Human   800 ----LYSV-----------------------LMGFAVEGSGLWPEEWYEGVCEIATKS---PLLT 834

  Fly   791 SSSSIKAAAAAAANSAASTPPATPTEALAANSNSKLQPLSDPSADLKVKERTLNLRLPGDSEGSE 855
            ..|.....:....|||......||.|  .:...|.:..|.||..::......|:..:       .
Human   835 FPSKEPLRSVLQYNSAMKNDTVTPAE--LSELRSTIINLLDPPPEVSALINKLDFAM-------S 890

  Fly   856 GALTKCSSINSIHKLKLPVPG---VVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAV 917
            ..|.....:..:..|:...|.   |:..:..:||     :||.:.|....:......||:
Human   891 TYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKA-----IQKDKSGMMQCVIAVADKVFD----- 945

  Fly   918 HYLFYSKEPGVLSFIGNKIFSFPDQE-----------------------VDLYIPQLV------- 952
                     ..|:.:.:|..:..::|                       .|.|:..||
Human   946 ---------AFLNMMADKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLL 1001

  Fly   953 ----VMYIQMDELAEVL----------DPYL-------------TIRCRKSV--DFSLKCLWLLE 988
                |:...:|.|..:.          .||.             |...|:|:  ||:.:|..:|:
Human  1002 WSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQ 1066

  Fly   989 ---------------------------------------------AYNYQVDSLGNSHNSSRKSK 1008
                                                         .||.|..:||.:..|.|.
Human  1067 EAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLSERP-- 1129

  Fly  1009 LALMKEIFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLS 1073
             |.:|:.:|        |.:.|......:.||...::..:..|...|..: .:::.|..|  .|.
Human  1130 -ACVKKDYS--------NFMASLNLRNRYAGEVYGMIRFSGTTGQMSDLN-KMMVQDLHS--ALD 1182

  Fly  1074 ISHRMYQQPPYTTQTL--------------PTTPAKLCLGDLT--SGHAFDNGCTCFE------- 1115
            .||     |.:.||.:              |.....||.|.|.  :.|..:....|:|
Human  1183 RSH-----PQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKD 1242

  Fly  1116 ----------------TVRGQVNGLLGQR-------TLCSCGAPKTSPQK--------EFMKALM 1149
                            ||. |..||....       .......||..|.:        :|:....
Human  1243 GVEVPFMREMAGAWHMTVE-QKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRF 1306

  Fly  1150 NVGKNLTSWPSKAEKTSAL---RMSLNL------INKNLPA---RVWL------PLYSD-IPHHV 1195
            .:.|..:|  .:.|..|:|   .||||:      :|:::.|   |..|      .|::| :|:..
Human  1307 EIAKYCSS--DQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNAT 1369

  Fly  1196 VR-ITEEK----------------------------------TAVLNSKDKTPYIIYVEVVEIPD 1225
            :| :..||                                  ||:.:.|   .|:.
Human  1370 IRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDK---KYLT--------- 1422

  Fly  1226 IYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLS--------CSRTSSCSSSQQGTSCRRKKGA 1282
              .|.|:|   |..:.|:|  :|| ..:.|.|..:        .|...|..|.:.|.|.:..:|:
Human  1423 --ASQLVP---PDNQDTRS--NLD-ITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGS 1479

  Fly  1283 DVD-----------------------------------GGCGDAGPHNSCSNVHSLGGLQFQED- 1311
            .:.                                   ...|:....|..|...||...|::::
Human  1480 QLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNV 1544

  Fly  1312 ----------------------------------------DV----------------------- 1313
                                                    ||
Human  1545 NLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHV 1609

  Fly  1314 --WSQEE--------------DEITAQY-LNMRKVSERDTI------------------------ 1337
              |:..:              ..:|||| :.:.:....|.|
Human  1610 LCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYIL 1674

  Fly  1338 ------------------SQISLDS---------CDSRDQ----------GPPVVF--------- 1356
                              :.|.||.         .|..||          ||...|
Human  1675 WAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFN 1739

  Fly  1357 ---NI---------GDVRLRHC----SNLSCENTKSFSNDPE-----------DPSAAALKEPWH 1394
               |:         ||.|.:.|    |.:..:......::||           .|..:|.|.|:.
Human  1740 KITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYL 1804

  Fly  1395 EKEKLIR-------------------ESSPY---GHLSNWRLLSAIVKCGDDLRQELMATQLLQM 1437
            .|.|:.|                   |.|..   |...:|:  :||.|.|||.||:::|.|::.:
Human  1805 AKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQ--AAIFKVGDDCRQDMLALQIIDL 1867

  Fly  1438 FKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSPSGESF 1502
            ||.|:|...:||:|.||::|..:...|:||.|.:..|..|:.:.::..:.:||..:||..|..:|
Human  1868 FKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAF 1932

  Fly  1503 RRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISP-KNLGFEQSPFKL 1566
            ::|:.||::|.|||.|:.:|||:||||||||:....||||||||||:...|| .|||:|.. .||
Human  1933 QQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPD-IKL 1996

  Fly  1567 TPEFVEVMGG-TSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNL 1630
            |.|.|.:||| ..:..::.|.::.:.|.::.|.:||.:::.|.:|.... ||||:   ..|::.|
Human  1997 TDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTG-LPCFR---GQTIKLL 2057

  Fly  1631 RKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1673
            :.||..|:||:|....:.:::|....|..::.||..|||.|.|
Human  2058 KHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDI 2100

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
fwd	NP_001356971.1	PI4Kc_III_beta	1385..1674	CDD:270712	113/313 (36%)
PI4KA	NP_477352.3	PI4K_N	378..1514	CDD:437106	210/1203 (17%)
		PI4Ka	1547..1722	CDD:238443	15/174 (9%)
		PI4Kc_III_alpha	1783..2101	CDD:270711	114/325 (35%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	1814..1820		0/5 (0%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	1954..1962		6/7 (86%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	1973..1997		13/24 (54%)