Protein Alignment fwd and Pi4kb

DIOPT Version :10

• Sequence 1: NP_001356971.1
• Gene: fwd / 45374
• FlyBaseID: FBgn0004373
• Length: 1682
• Species: Drosophila melanogaster
• Sequence 2: NP_001280644.1
• Gene: Pi4kb / 107650
• MGIID: 1334433
• Length: 816
• Species: Mus musculus
• Alignment Length: 951
• Identity: 367/951 - (38%)
• Similarity: 533/951 - (56%)
• Gaps: 171/951 - (17%)

  Fly   749 QPDHSGTVSLVGSPNGGSSV--------------PQDLVKSPTDISKKKEFFSLLTSSSSIKAAA 799
            :|....|.|..|: ||||.:              |:...|:..::.:|            :|...
Mouse    12 KPTSEPTPSPSGN-NGGSLLSVITEGVGELSVIDPEVAQKACQEVLEK------------VKLLH 63

  Fly   800 AAAANSAASTPPATPTEALAANS-NSKLQPLSDPSADLKVKERTLNLRLPGDSEGSEGALTKCSS 863
            ...|.|:    ..||.|.:..:. :::::.|.||.|.::.:|..:.   .|.:.|:.....:...
Mouse    64 GGVAISS----KGTPLELVNGDGVDNEIRCLDDPPAQIREEEDEMG---AGVASGTAKGARRRRQ 121

  Fly   864 INSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAVHYLFYSKEPGV 928
            .||..                              ||.|||.|||.:|:|..|:.||:.||||||
Mouse   122 NNSAK------------------------------QSWLLRLFESKLFDISMAISYLYNSKEPGV 156

  Fly   929 LSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDE-LAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNY 992
            .::|||::|.|.:::||.|:|||:.|||.||| :.:.:.||:..|||:|::|||:|..||.||:.
Mouse   157 QAYIGNRLFYFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSS 221

  Fly   993 QVDSLGNSHNSSRK-SKLALMKEIFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQ 1056
            .:......|:...| .||.|..|:  |..|::.:....|..        |.:.::.:|:||.||:
Mouse   222 DMHISTQRHSRGTKLRKLILSDEL--KPAHRKRELPTLSPA--------PDTGLSPSKRTHQRSK 276

  Fly  1057 SDATVLLADFRSPHTLSIS---HRMYQQPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVR 1118
            ||||.         ::|:|   .|....|....:....:         :|..:.||
Mouse   277 SDATA---------SISLSSNLKRTASNPKVENEDEELS---------SSTESIDN--------- 314

  Fly  1119 GQVNGLLGQRTLCSCGAP-KTSPQKEFMKALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPAR 1182
                         |..:| :.:|::||:|:||.:||.|.:.|:|.:||..|...|:|:|..||||
Mouse   315 -------------SFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPAR 366

  Fly  1183 VWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPS--LRHTKSE 1245
            ||||. :...|||||:...:..|||||||.||:|||||:|..:..|:. :|..:|.  :|.|:|.
Mouse   367 VWLPT-AGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTS-VPARIPENRIRSTRSV 429

  Fly  1246 EHLDGSCLNSHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQE 1310
            |:|. .|..:|:.                               .||..::..|..:       :
Mouse   430 ENLP-ECGITHEQ-------------------------------RAGSFSTVPNYDN-------D 455

  Fly  1311 DDVWSQEEDEITAQYLNMRKV--SERDTISQISLDSCDSRDQGPPVVFNIGDVRLRHCSNLSCEN 1373
            |:.||  .|:|....:.:.:|  :..|.|||.|:||..|::...||....||:|.|....|:...
Mouse   456 DEAWS--VDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTP 518

  Fly  1374 TKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMF 1438
            | :|..|||||||.||||||.||.:.|||.||||||.||||||.||||||||||||:|.|:|:..
Mouse   519 T-AFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQL 582

  Fly  1439 KIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSPSGESFR 1503
            :.||::|:|.||::||||:.:|.|||:|||::|.||:||:||.|..||.:||:.|:||.:.|:|.
Mouse   583 QSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFL 647

  Fly  1504 RAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPKNLGFEQSPFKLTP 1568
            .||:|||||||.|||:.||||||||||||||..::|||||||||||||.||:|||||.|.||||.
Mouse   648 SAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTT 712

  Fly  1569 EFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNLRKR 1633
            |||:||||.:.:.:..:..|:|.|:::||||||:::..||||:..:.||||..  |.|::||::|
Mouse   713 EFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHG--SSTIRNLKER 775

  Fly  1634 FHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGIL 1674
            |||::||::::..|||:|..|::|::||||||:||.||||:
Mouse   776 FHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM 816

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
fwd	NP_001356971.1	PI4Kc_III_beta	1385..1674	CDD:270712	176/288 (61%)
Pi4kb	NP_001280644.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..30		8/18 (44%)
		Interaction with ACBD3. /evidence=ECO:0000250|UniProtKB:Q9UBF8	2..68		12/68 (18%)
		PI4KB_NTD	2..66	CDD:412074	12/66 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	101..120		3/21 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	250..318		19/115 (17%)
		PI4Kc_III_beta	529..816	CDD:270712	176/288 (61%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	541..547		2/5 (40%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	668..676		7/7 (100%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	687..711		19/23 (83%)