Protein Alignment fwd and pi4ka

DIOPT Version :10

• Sequence 1: NP_001356971.1
• Gene: fwd / 45374
• FlyBaseID: FBgn0004373
• Length: 1682
• Species: Drosophila melanogaster
• Sequence 2: XP_031761716.1
• Gene: pi4ka / 100127709
• XenbaseID: XB-GENE-980714
• Length: 2102
• Species: Xenopus tropicalis
• Alignment Length: 2193
• Identity: 424/2193 - (19%)
• Similarity: 714/2193 - (32%)
• Gaps: 759/2193 - (34%)

  Fly    57 PVVVVAEERPVSKAGSPSTVATMATGVDVVDHCLPTTVRLSVTEALSDLNMNGKAVEPPVTPCTS 121
            |.|...||....|......||      :....||   |.|....|..||:...:.:|
 Frog    91 PKVQWIEESNSRKGKGTLPVA------ETFSFCL---VTLLSDVAHRDLSFREQILE-------- 138

  Fly   122 VGDNANLEVAE--TWACPPPQATDDEDISEHSTAACTSTSSS-SEHSNSTVVHSPTVGESTCDSV 183
                |.|:|.:  ...|..||..|.|.:.:::.......|.: ..:||:.        ||....:
 Frog   139 ----AVLDVMQCLLGMCQSPQIQDKEYLCKYAVPCLIGISRAFGRYSNTE--------ESLLSKL 191

  Fly   184 SVSVSHPSPSVSQ-----NNTSSATGRMSSPHSQSSLSIACEPHSASTTTLS-SLGSDL------ 236
            ...||..|....:     ...|....|...|   |||...|:..|......| |.||.:
 Frog   192 FPKVSPQSLKAPEELEGIRRRSFNDFRSILP---SSLLTVCQGDSLKRKASSVSSGSQVSPDRIV 253

  Fly   237 ---GNGNGSTVPAFLMAAPVTLPIGGQMPTIKCLPGASSAASTP------KHSRYSKPRLSLSRV 292
               |:..|||:|.|   ....||.|..:.....|...||:.|..      .:..:..|:.:|.::
 Frog   254 PPPGSPAGSTIPYF---EGSYLPDGSAVDPDYYLTTISSSFSVSPLFTGVNNKEFDMPQDTLRQL 315

  Fly   293 FNHSMPSVHGRPNLSLAPAGVPGSVNPPKRISTHQRNLS--LDFRSMGILLPPVSQVTNNHINHT 355
            . |.:..:...|.||.....:.........:..:.:..|  |......:|...:..|.:.|.:..
 Frog   316 L-HMVKQIVADPFLSSLDMLISEVTEANPSVDLYYKTFSDPLYVAVFKMLRDTLYNVKDPHTSFV 379

  Fly   356 QH------HRNRSLDSALQRIPEVEVSSPNAE--------SENTFCSPSILGA--------TMCS 398
            :.      .:..|....||:|.. ::...|.|        ..|..|...::.|        .:|.
 Frog   380 KEIHDFVLEQFNSSQGELQKILH-DMDRLNNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCI 443

  Fly   399 KI-----ATTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQSISLAAACRSSA 458
            |:     :.|::.|..|..|        :...|...:|.:  ..:.||..|    |:..:.|...
 Frog   444 KLSEKLQSKTSSKVIIAHMP--------LLICCLQGLGRL--CERFPVVVH----SVTPSLRDFL 494

  Fly   459 VVESCPTLRM-RPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGS-TKKREDLTSLGSDD 521
            |:.|...::: :..|.....||     .:.:|.|...|.|:.|..|...| ....|.|..:..|:
 Frog   495 VIPSPVLVKLYKYHSQYQTGAN-----DIKISVTNEHSESTMNIMSGKKSQASMYEQLRDIAIDN 554

  Fly   522 SGIIC-----GSESDQISL--------NRICHSHESLDSGEMDADADAEEE-------CVDLMDT 566
               ||     |...|.:.:        ||:..|.|:    |.||....:..       .|.|.||
 Frog   555 ---ICRCLKAGLTIDPVIVEAFLASLSNRLYISQEN----EKDAHLIPDHTIRALGHIAVALRDT 612

  Fly   567 TSIDEEYNIMQP------DHLCLYPPQ---AASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQ 622
            .      .:|||      ...|..|.|   ....:|.|..:..::...::...:.::.::.:.:.
 Frog   613 P------KVMQPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSV 671

  Fly   623 IES--EDSVDAGVDARTRYRVMNMSQAAINVELGTASN-----EID-----------------PD 663
            :.|  :|..|.|      ||  :.|.|.||.....|:|     :||                 ..
 Frog   672 VYSATKDYKDHG------YR--HCSLAVINALANIAANIQGEHQIDDLLMNLLELFVQLGLEGKR 728

  Fly   664 VNYRESHQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNAIF 728
            .:.|.|.:.|...|.|    |..:.....|.:.......|...|:....|.||::|:
 Frog   729 ASERASEKGPALKASS----SAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFW-------- 781

  Fly   729 RTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSV-PQDLVKSPTDISKKKEFFSLLTSS 792
              ..|:                       |:|....||.: |::..:...:|:.|.   .|||..
 Frog   782 --LYSV-----------------------LMGFAVEGSGLWPEEWYEGVCEIATKS---PLLTFP 818

  Fly   793 SSIKAAAAAAANSAASTPPATPTE--ALAANSNSKLQPLSDPSADLKVKERTLNLRLPGDSEGSE 855
            |.....:....|||......||.|  .|.:...:.|.|..:.||.:...:..::..|.
 Frog   819 SKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPAPEVSALINKLDFAMSTYLL------- 876

  Fly   856 GALTKCSSINSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAV--- 917
             ::.:...:..:|........|:.::..:||     :||.:.|....:......||.....|
 Frog   877 -SVYRLEHMRVLHATDKDRFQVMFFYFEDKA-----IQKDKSGMMQCVIAVADKVFEAFLNVMAD 935

  Fly   918 -------------HYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLV-----------VMYIQM 958
                         |..|.     :::|  |.|.....:..|.|:..||           |:...:
 Frog   936 KPKTKENEEELERHAQFL-----LVNF--NHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTML 993

  Fly   959 DELAEVL----------DPYL-------------TIRCRKSV--DFSLKCLWLLE---------- 988
            |.|..:.          .||.             |...|:|:  ||:.:|..:|:
 Frog   994 DILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPSVT 1058

  Fly   989 -----------------------------------AYNYQVDSLGNSHNSSRKSKL----ALMKE 1014
                                               .||.|..:||:..:|::.::|    |.:|:
 Frog  1059 KSHLQEYLNKHQNWMSGLSQHTGLAMATESILHFAGYNRQNTTLGSPASSAQVTQLTERPACVKK 1123

  Fly  1015 IFSKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISH--- 1076
            .:|        |.:.|......:.||...::..         ||||...:|.   :.|.:.|
 Frog  1124 DYS--------NFMASLNLRNRYAGEVAGMIEF---------SDATGRTSDL---NRLMLKHLGD 1168

  Fly  1077 -RMYQQPPYTTQTLPTTPA--------------KLCLGDLT--SGHAFDNGCTCFETVRGQVNGL 1124
             ....||...||.:....|              .||...|.  :.|..:....|::.:....||:
 Frog  1169 ALDCGQPESYTQVMFKLTALLISSKECDAQLLHHLCWAPLRMFTDHGMETAIACWDWLLAAKNGI 1233

  Fly  1125 -------------------LG------------------QRTLCSCGAPKTSPQK---EFMKALM 1149
                               :|                  |...|   ||:.:|..   :|:....
 Frog  1234 EVPFMREMAGAWQMTVEQKIGLFSAEKKEADPLAASEQSQPRPC---APQVNPHYIWIDFLMQRF 1295

  Fly  1150 NVGKNLTSWPSKAEKTSAL---RMSLNL------INKNLPA---RVWL------PLYSD-IPHHV 1195
            .:.|..:|  .:.|..|:|   .:|||:      :|:::.|   |..|      .|::| :|:..
 Frog  1296 EIAKYCSS--DQVEIFSSLLQRSLSLNIGGCKGSLNRHVAAIGPRFKLLTLGLSLLHADVVPNAT 1358

  Fly  1196 VR-ITEEK----------------------------------TAVLNSK------------DKTP 1213
            :| :..||                                  ||:.:.|            ::.|
 Frog  1359 IRNVLREKIYSTAFDYFSCPPKFPTQTQKLLREDISIMIKFWTAMFSDKKYLTANQLVPPDNQDP 1423

  Fly  1214 YIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLSCSRTSSCSSSQQGT---- 1274
            .:..:.|:.  |:..|.|  .:....|...::..::...|:|.......::.....:.:||
 Frog  1424 SVNSLSVMS--DVNRSNL--DVTVGSRQQTAQGWINTYPLSSGMSTISKKSGLAKKTNRGTQLHK 1484

  Fly  1275 --------------------------------------------------SCRRKKGADVDGGCG 1289
                                                              |...|:..|......
 Frog  1485 YYMKRRTLLLSLLATEIERLTTWYNPLSSPELELDQTGENSVANWRSKYISLSEKQWKDNVNLAW 1549

  Fly  1290 DAGPH------NSCSNVHSLGG------------------------------------------- 1305
            ...||      ....|..::||
 Frog  1550 SISPHLALQLPTRFKNTEAIGGEVTRLVRLDPGAVSDTPEAIKYLVTWHTIDADAPELSHILCWT 1614

  Fly  1306 -----------------------------------------------LQF------QEDDVWSQE 1317
                                                           |::      :|..:|:..
 Frog  1615 PTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAA 1679

  Fly  1318 EDEITA-QYL-NMRKVSERDTISQISLDSCDSRDQGPPV-------------------------- 1354
            :.::.| |:: ||:        :.|.||. :...:.|.:
 Frog  1680 KSQLLAHQFIWNMK--------TNIFLDE-EGHQKDPDIGELLEQLVEEITGSLSGPAKDFYQRE 1735

  Fly  1355 ------VFNI---------GDVRLRHCSNLSCENTK-----SFSNDPE-----------DPSAAA 1388
                  :.|:         |:.|.:.|.| |....|     ...::||           .|..:|
 Frog  1736 FDFFNKITNVSAIIKPFPKGEERKKACLN-SLSEVKVQPGCYLPSNPEAIVLDIDYTSGTPLQSA 1799

  Fly  1389 LKEPW-------------HEKEKL------IRESSPYGHLSN--WRLLSAIVKCGDDLRQELMAT 1432
            .|.|:             .|||.|      |.||...|....  |:  :||.|.|||.||:::|.
 Frog  1800 AKAPYLAKFKVKRCGVSELEKEGLSCRSDSIDESEEEGGDGKVCWQ--AAIFKVGDDCRQDMLAL 1862

  Fly  1433 QLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKKNSNKSLKEYFIDEYGSP 1497
            |::.:||.|:|...:||:|.||::|..:...|:||.|.:..|..|:.:.::..:.:||..:||..
 Frog  1863 QIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDE 1927

  Fly  1498 SGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISP-KNLGFEQ 1561
            |..:|::|:.||::|.|||.|:.:|||:||||||||:....||||||||||:...|| .|||:|.
 Frog  1928 SALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEP 1992

  Fly  1562 SPFKLTPEFVEVMGG-TSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSG 1625
            . .|||.|.|.:||| ..:..::.|.::.:.|.::.|.:||.:::.|.:|.... ||||:   ..
 Frog  1993 D-IKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTG-LPCFR---GQ 2052

  Fly  1626 TVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1673
            |::.|::||..|.||:|....:.:::|:...|..::.||..|||.|.|
 Frog  2053 TIKLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDI 2100

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
fwd	NP_001356971.1	PI4Kc_III_beta	1385..1674	CDD:270712	115/312 (37%)
pi4ka	XP_031761716.1	PI4K_N	361..1515	CDD:437106	216/1283 (17%)
		PI4Ka	1548..1723	CDD:238443	16/183 (9%)
		PI4Kc_III_alpha	1784..2101	CDD:270711	116/324 (36%)