Protein Alignment Cnx99A and Calr

DIOPT Version :10

• Sequence 1: NP_477157.1
• Gene: Cnx99A / 44643
• FlyBaseID: FBgn0015622
• Length: 605
• Species: Drosophila melanogaster
• Sequence 2: NP_031617.1
• Gene: Calr / 12317
• MGIID: 88252
• Length: 416
• Species: Mus musculus
• Alignment Length: 543
• Identity: 159/543 - (29%)
• Similarity: 233/543 - (42%)
• Gaps: 152/543 - (27%)

  Fly    60 LAYESPVIDAKKFHFADHFDDVEESRKRWVLSQAKKD--DIAEEISKYDGIWNWESPQRIVWAND 122
            ||...|.|     :|.:.|.|.:....|||.|:.|.|  .......|:.|...          .|
Mouse    14 LAAADPAI-----YFKEQFLDGDAWTNRWVESKHKSDFGKFVLSSGKFYGDLE----------KD 63

  Fly   123 LGLVLKSKAKHAAIAAPLRKPFEFKSDKPLVVQYEVTLQEGQECGGSYLKLLSAGKDTEQLKAFN 187
            .||.....|:..|::|.. :||..|. :.||||:.|..::..:|||.|:||..:|.|.   |..:
Mouse    64 KGLQTSQDARFYALSAKF-EPFSNKG-QTLVVQFTVKHEQNIDCGGGYVKLFPSGLDQ---KDMH 123

  Fly   188 DKTPYTIMFGPDKCG-NDVKMHFIFRHVNPINGTITEKHCNKPKN-------RLEEPFKDKLPHL 244
            ..:.|.||||||.|| ...|:|.||.:              |.||       |.::   |:..||
Mouse   124 GDSEYNIMFGPDICGPGTKKVHVIFNY--------------KGKNVLINKDIRCKD---DEFTHL 171

  Fly   245 YQLVVRPDNSFEIRVDHKIINEGSLLTDFKPPVNPPAEIDDPNDHKPESWDEREKIPDPTAHKPE 309
            |.|:|||||::|:::|:..:..|||..|:  ...||.:|.||:..|||.||||.||.|||..|||
Mouse   172 YTLIVRPDNTYEVKIDNSQVESGSLEDDW--DFLPPKKIKDPDAAKPEDWDERAKIDDPTDSKPE 234

  Fly   310 DWDEDAPPQLPDTDAVMPNGWLEDEPDMIFDPTATKPEDWDAEIDGEWEAPLVDNPVCEKAPGCG 374
            ||                     |:|:.|.||.|.||||||.|:|||||
Mouse   235 DW---------------------DKPEHIPDPDAKKPEDWDEEMDGEWE---------------- 262

  Fly   375 KWKAPLIPNPNYKGKWRAPMIENPNYQGKWAPRKIPNPDFFEDLKPFQMTPISAVGLELWSMSSD 439
               .|:|.||.|||:|:...|:||:|:|.|...:|.||::..|...:.....:.:||:||.:.|.
Mouse   263 ---PPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDANIYAYDSFAVLGLDLWQVKSG 324

  Fly   440 ILFDNLIITDDVEVARDFAANSFDIKRRYIDRESDSFVNKVVELAKANPSIWGIGLVAIVALVAL 504
            .:|||.:||:|...|.:|...:                             ||:
Mouse   325 TIFDNFLITNDEAYAEEFGNET-----------------------------WGV----------- 349

  Fly   505 TIYCRFGTAKSQDSAAKKAAAEAKKSDDPQPDDEPEAEEESDERAAGDTSKESTPLSASPKKNQK 569
                    .|:.:...|....|.::..:.:.|.:.:.|||::::...|...|.
Mouse   350 --------TKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDDDRDED------------ 394

  Fly   570 SDLDDNEEESKAAESREPAQTEE 592
               :|.|:|.:..|...|.|.::
Mouse   395 ---EDEEDEKEEDEEESPGQAKD 414

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cnx99A	NP_477157.1	Calreticulin	74..447	CDD:459737	133/382 (35%)
Calr	NP_031617.1	N-domain	18..197		67/215 (31%)
		Calreticulin	23..332	CDD:459737	133/382 (35%)
		4 X approximate repeats	191..255		37/86 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	193..277		52/125 (42%)
		P-domain	198..308		59/151 (39%)
		Interaction with PPIB. /evidence=ECO:0000269|PubMed:20801878	237..270		21/51 (41%)
		3 X approximate repeats	259..297		19/56 (34%)
		C-domain	309..416		30/169 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	350..416		15/80 (19%)
		Prevents secretion from ER	413..416		0/2 (0%)