Protein Alignment Shark and BTK

DIOPT Version :10

• Sequence 1: NP_524743.2
• Gene: Shark / 44353
• FlyBaseID: FBgn0015295
• Length: 939
• Species: Drosophila melanogaster
• Sequence 2: NP_001274273.1
• Gene: BTK / 695
• HGNCID: 1133
• Length: 693
• Species: Homo sapiens
• Alignment Length: 826
• Identity: 189/826 - (22%)
• Similarity: 307/826 - (37%)
• Gaps: 236/826 - (28%)

  Fly   159 HLAALHSDEDILKHLLNAKVQVNSSDSFGCQPLHYAARSKPASFIRTLISAQANVQGRNIDNGYV 223
            |...|..:|.:...:|.:.....|.......||::    |...|:.|:  .:.:....:.:.|
Human    25 HTGELQKEEAMAAVILESIFLKRSQQKKKTSPLNF----KKRLFLLTV--HKLSYYEYDFERG-- 81

  Fly   224 PLHEAAKHGNLE-----AVQELLLAEAPP----LPR--TSSGE-----------FPFDLAKEAGQ 266
              ...:|.|:::     .|:.::..:.||    :||  ..|.|           :||.:..:.|.
Human    82 --RRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGP 144

  Fly   267 TAVEEFLLNYKLPPANTTRDQWYH--GTLTREEAVAILKKHAKELLAKQPEVDTSGCFLV--RYS 327
            .        |...|....|.:|.|  ..:.|..:..:.|.|.              ||.:  :|
Human   145 L--------YVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHP--------------CFWIDGQY- 186

  Fly   328 ESPAASGLVLTLLCDQVVKNFRISQADLYQNGNKVQSGGSKFLYIDDGPYWPSVEHLIAHFMRFS 392
                       |.|.|..||                :.|.:.|...:|...|...|.
Human   187 -----------LCCSQTAKN----------------AMGCQILENRNGSLKPGSSHR-------- 216

  Fly   393 YGLPVSLKYPVPPQPK---------PEVPSFATIPRSNMKPKAA--------------------- 427
                 ..|.|:||.|:         |..|:.|.:..|.:|...|
Human   217 -----KTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYF 276

  Fly   428 --SPATPPTPVSPHSHHQHPHVPALTITKKKQKENSSSMFNTLRLTSPKKALFDMNSLRKNKSKG 490
              ..:..|...:...:.|..::|:..:|   :.|:|..|:...     .|.:....:.:..|.:|
Human   277 ILEESNLPWWRARDKNGQEGYIPSNYVT---EAEDSIEMYEWY-----SKHMTRSQAEQLLKQEG 333

  Fly   491 KRSD---SESSVSGSLAGTEQELQAAAPMLKSLSFSTEFSTFNADGVTGSGAAAAGEVYNVPRNN 552
            |...   .:||.:|...                     .|.|         |.:.|:...|.|:.
Human   334 KEGGFIVRDSSKAGKYT---------------------VSVF---------AKSTGDPQGVIRHY 368

  Fly   553 TPIEIDLPPIAQKTEAEVEYFTKSDVAIERERAGQWIGNGYQPTMDVLSLLDQQIKAPAVARLNS 617
            .   :...|.:|...||...|:                     |:..|....|...|..::||
Human   369 V---VCSTPQSQYYLAEKHLFS---------------------TIPELINYHQHNSAGLISRL-- 407

  Fly   618 LGPNASTESEMASY-LHRKCSGTPSTPSATEVEAAKLRF---FIEPEKLVLDREIGHGEFGSVHS 678
                        .| :.::....|||        |.|.:   .|:|:.|...:|:|.|:||.|..
Human   408 ------------KYPVSQQNKNAPST--------AGLGYGSWEIDPKDLTFLKELGTGQFGVVKY 452

  Fly   679 G-WLLRKSGAGEESRLEVAIKMLSDEHSNKQEFLREASVMMRLEHKCIVRLIGI-AKGEMLMMVQ 741
            | |         ..:.:|||||:.:...::.||:.||.|||.|.|:.:|:|.|: .|...:.::.
Human   453 GKW---------RGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIIT 508

  Fly   742 ELAPLGSMLQYILDHGHEITANAELKVWASQIACGMHYLESQHFVHRDLAARNILLTARHQAKIS 806
            |....|.:|.|:.:..|.......|:: ...:...|.||||:.|:||||||||.|:..:...|:|
Human   509 EYMANGCLLNYLREMRHRFQTQQLLEM-CKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVS 572

  Fly   807 DFGMSRSLRPGSTEYQFTQGGRWPIRWYAPESFNLGIFSHASDVWSFGVTIWEMFSLGAPPYGEI 871
            |||:||.:.  ..||..:.|.::|:||..||......||..||:|:|||.:||::|||..||...
Human   573 DFGLSRYVL--DDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERF 635

  Fly   872 SNVDAIKLVDSGERLPQPNLCPAYIYAVMQSCWKERPKDRPTFVYL 917
            :|.:..:.:..|.||.:|:|....:|.:|.|||.|:..:||||..|
Human   636 TNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKIL 681

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Shark	NP_524743.2	SH2_Nterm_shark_like	8..91	CDD:198210
		ANKYR	<94..317	CDD:440430	31/181 (17%)
		ANK repeat	124..151	CDD:293786
		ANK repeat	153..184	CDD:293786	5/24 (21%)
		ANK repeat	186..217	CDD:293786	5/30 (17%)
		ANK repeat	220..245	CDD:293786	4/29 (14%)
		SH2_Cterm_shark_like	287..395	CDD:198211	18/111 (16%)
		PTKc_Syk_like	666..926	CDD:270650	94/254 (37%)
BTK	NP_001274273.1	PH_Btk	40..200	CDD:269944	37/219 (17%)
		SH3_BTK	251..305	CDD:212839	6/56 (11%)
		SH2_Tec_Btk	308..413	CDD:198260	27/177 (15%)
		PTKc_Btk_Bmx	431..686	CDD:173657	97/263 (37%)