Protein Alignment Ekar and Grin2b

DIOPT Version :10

• Sequence 1: NP_001036250.1
• Gene: Ekar / 43806
• FlyBaseID: FBgn0039916
• Length: 899
• Species: Drosophila melanogaster
• Sequence 2: NP_036706.1
• Gene: Grin2b / 24410
• RGDID: 2738
• Length: 1482
• Species: Rattus norvegicus
• Alignment Length: 932
• Identity: 220/932 - (23%)
• Similarity: 374/932 - (40%)
• Gaps: 242/932 - (25%)

  Fly    20 PPLVRIGAIFSNQPGMYNSELAFRYA-----IHRLNMDKSLLPETTVDYYVEYV--NRFDSFETV 77
            ||.:.|..|....    :.|:|.:.|     .|.|    |::|.      ||.|  |..|....:
  Rat    32 PPSIGIAVILVGT----SDEVAIKDAHEKDDFHHL----SVVPR------VELVAMNETDPKSII 82

  Fly    78 QKVCKLIR-VGVQAVFSPTDSVLATHINSICDALDI-------PNIGRSAHDFSINVYPSKQLVN 134
            .::|.|:. ..:|.|....|    |...:|...||.       |.:|  .|..|..:...|...:
  Rat    83 TRICDLMSDRKIQGVVFADD----TDQEAIAQILDFISAQTLTPILG--IHGGSSMIMADKDESS 141

  Fly   135 YAFN-------------DVIQYLNWTRFGILHEKENG---IIN--LHQLSRSFHG----EVHMRQ 177
            ..|.             ::::..:|..|.|:.....|   .:|  ...:..||.|    ||.:..
  Rat   142 MFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLD 206

  Fly   178 VSRDSYVSAL-NEFKGKEIHNIIIDTNSNGISILLKNILQQQMNEYKYHYLFTSF---DLETYDL 238
            :|.|...|.: |:.|..:...|::.......:.:.:......:..|.|.::..|.   |.:|...
  Rat   207 MSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPS 271

  Fly   239 EDFKYNFVNITSFRLVDTADVGVKQILKD---------IGLYSHHIF----KKPYLNLHIKKSTI 290
            | |....::::    .|..|.|:...::|         ..:.|.|.|    |....|.|.|:
  Rat   272 E-FPTGLISVS----YDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKR--- 328

  Fly   291 LESEPALMFDSVYVFAIGLQTLEQSHSLT--LLNISCEEENSWDGGLSLINYLNAVEWKGLTGPI 353
                       :|          ||:.|.  |:|::.|..|                       :
  Rat   329 -----------IY----------QSNMLNRYLINVTFEGRN-----------------------L 349

  Fly   354 QF-KDGQRVQFKLDLIKL-KQHSIVKVGEWTPHG---------------------HLNITEPSMF 395
            .| :||.::..||.:|.| |:....:||:|....                     ||:|
  Rat   350 SFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSI------ 408

  Fly   396 FDAGSMNVTLVVITILETPYVMMH--------------------YGKNFTGNERFY------GFC 434
                        :|:.|.|:|::.                    ..:|.|..|..|      |||
  Rat   409 ------------VTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFC 461

  Fly   435 VDILETISREVGFDYILDLVPDRKYGAKDPETGEWNGMVAQLMKYKADLAVGSMTITYARESVID 499
            :|||:.||:.|.|.|.|.||.:.|:|.|  ..|.||||:.:::..:|.:||||:||...|..|:|
  Rat   462 IDILKKISKSVKFTYDLYLVTNGKHGKK--INGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVD 524

  Fly   500 FTKPFMNLGISILFKVP--TSEPTRLFSFMNPLAIEIWIYVLIAYFLVS-LCIYIVGKLSPIEWK 561
            |:.||:..|||::....  |..|:   :|:.|.:.::|:.:.:...:|| :.:::....||:.:
  Rat   525 FSVPFIETGISVMVSRSNGTVSPS---AFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGY- 585

  Fly   562 CINACDLENISIGN-QFSLTDSFWFTIG-TFMQQSPDIYPRAMSTRIISSTWGFFSLIIVASYTA 624
              |.|..:....|. .|::..:.|...| .|....|...|:..:::|:.|.|.||::|.:|||||
  Rat   586 --NRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTA 648

  Fly   625 NLAAFLTTERMINPIENAEDLASQ------TEISYGTLDSGSTMTFFRDSVIETY----KKIWRS 679
            |||||:..|..::.:....|...|      ....:||:.:|||....|::..|.:    |...|.
  Rat   649 NLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRG 713

  Fly   680 MDNKKPSAFTTTYEDGIKRVNQGNY-AFLMESTMLDYIVQRD--CNLTQIGG--LLDTKGYGIAT 739
            :|            |.:..:..|.. ||:.::.:|:|:..||  |.|..||.  :..:.|||||.
  Rat   714 VD------------DALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAI 766

  Fly   740 PKGSPWRDKISLAILELQERGDIQMLYDKWWKNTDETCTRKNTSKQSKANSLGLESIGGVFVVLI 804
            .|.|.|:.::.||||:|...|:::.|...|.     |....|...:..::.|.::::.|||.:|.
  Rat   767 QKDSGWKRQVDLAILQLFGDGEMEELEALWL-----TGICHNEKNEVMSSQLDIDNMAGVFYMLG 826

  Fly   805 AGIIVAAVVAFFE--FWYNFRY 824
            |.:.::.:....|  |::.||:
  Rat   827 AAMALSLITFICEHLFYWQFRH 848

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ekar	NP_001036250.1	PBP1_iGluR_Kainate	24..390	CDD:380605	84/444 (19%)
		PBP2_iGluR_Kainate	402..771	CDD:270432	121/414 (29%)
		Lig_chan	532..806	CDD:459656	78/291 (27%)
Grin2b	NP_036706.1	PBP1_iGluR_NMDA_NR2	33..388	CDD:380601	83/426 (19%)
		PBP2_iGluR_NMDA_Nr2	403..803	CDD:270436	125/442 (28%)
		Pore-forming. /evidence=ECO:0000269|PubMed:24876489, ECO:0000305|PubMed:24876489	604..623		4/18 (22%)
		NMDAR2_C	840..1482	CDD:463148	3/9 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1161..1194
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1269..1301
		Interaction with DAPK1. /evidence=ECO:0000250|UniProtKB:Q01097	1292..1304
		PDZ-binding	1480..1482