Protein Alignment Smg6 and ESL2

DIOPT Version :10

• Sequence 1: NP_651321.1
• Gene: Smg6 / 42994
• FlyBaseID: FBgn0039260
• Length: 948
• Species: Drosophila melanogaster
• Sequence 2: NP_013022.1
• Gene: ESL2 / 853971
• SGDID: S000001804
• Length: 1195
• Species: Saccharomyces cerevisiae
• Alignment Length: 1218
• Identity: 201/1218 - (16%)
• Similarity: 397/1218 - (32%)
• Gaps: 450/1218 - (36%)

  Fly    18 TLPKRLQNKIAASCSHAAAAATTTKALETEN---------------AENAEPVA----TTANASR 63
            |.|:|...|.:.........:::.:||:.||               :||...:|    :..|..:
Yeast    95 TYPERTTVKESEKNPSPRYVSSSKRALKRENSVGITQSSALISKSFSENGGSIAHEKWSPENMIK 159

  Fly    64 PVEILSN--ASTGGGKPDAQSKQPVLDTSGKMAWEGGIPGILRVTT---------SVAGDVNRNG 117
            |:.:..|  |....|. |.|||..:            :.|..|.:.         :.|.|.:.|.
Yeast   160 PLNVSQNSLAFVDAGS-DEQSKSEI------------VGGFQRKSNNSQEINDKDNSARDQDFNN 211

  Fly   118 QGQGKNKSGNSARRRGRKD--------------KEHATQSPVTRTRLTTEHPDWFEPLLGLPHAK 168
            .|...|.:.:|:......:              :::...|..:..|   |..|..:|
Yeast   212 SGNNNNNNNHSSNNNDNNNNNNDDNNNNNNSNSRDNNNNSDDSNER---EENDSCKP-------- 265

  Fly   169 NIVHLYDQLMVLNQRNLILINWKNFCYIHDQLQDAFKQLLLEQLKFV--CEHKVD------VFFW 225
                      ..|:|:.|.:..|        ||:.:|.::.::::..  |....:      ...|
Yeast   266 ----------ASNKRSGIALIQK--------LQELYKVIVKQEIELQERCSQLTNSQTTELKSLW 312

  Fly   226 KLLFYN---VRNYLKRQQT---DQAHTHTLLLIEQAIKFYRM-----LYD--------KLMAKCV 271
            .:...|   |.||:....|   .....|.|::.::.::.||:     :|.        |..:..:
Yeast   313 TIYKINTDLVNNYVTFITTALLPSQPPHDLVIGQEIVEIYRIERRLWVYGTITFLDVLKNFSNFM 377

  Fly   272 ASSRCDSALKVVAQRL---------------LICLGDLTRYRVNHVKAT--DY-MEAARYYQRAQ 318
            ....|...:..|...|               |..||||:|..:....::  |: :.|..:|..|.
Yeast   378 DPEVCCQFITHVFVSLSTMISDIPSKYSITWLQRLGDLSRMAIALYPSSFIDWKLSAEHWYTEAM 442

  Fly   319 ELVPGNGAPFNQLAVI-------------SIYHHKRFDAVYYYVRSLLTSNSIQSA--------- 361
            :.:..:|..:..::.:             |::..:.|.....|:: |:..|..|.|
Yeast   443 KYIYNHGKLYYHMSTVQQNTLEAFVNLGKSVFCQETFTPSPQYMQ-LVIDNIYQRAFVERNNGNL 506

  Fly   362 KESLL-------------------DLFDEIRRKYEE----------------------------- 378
            :.|||                   ||.:.:...:.|
Yeast   507 RNSLLIEYLKHSEAMLLPSFLESPDLQNVVLSYFIEKFGIDANGCNIFNAEDMFVQNPDFFKYFF 571

  Fly   379 ---TEMKQSPMHYAAG-----------------SKNQKSKQMRKEVWIYPDGIRRLHRTDDKGNK 423
               ....||.:....|                 .|.:|.|:.||                    |
Yeast   572 RHGPSFAQSHILQIVGFGEPKNPFAILFELPKYLKERKDKKERK--------------------K 616

  Fly   424 AKGNKATMAEV----NRYEEMPPEEILPRIVSLYLY-LLGKLYTGTDVDSL-YPLLRKLQIQIGV 482
            :..|.:::.|.    :|.:....:||:....|:..: ||.:.:  .|:|:| .|:|..:
Yeast   617 SSNNDSSVTESSTGNSRNDNEDDDEIMSSTTSISDHDLLAEFF--NDIDTLRRPILPSM------ 673

  Fly   483 ALRHENLLSRSKLLKIVALNLFVV--------------EHNKP----------KTSRREMRYHSF 523
             |.:|..|...|.|.:.:|...::              .|..|          |:|:.     |.
Yeast   674 -LTNEAWLETLKFLNMTSLKCGIIVLRKFLHGPLGIALPHILPWIYFIISICLKSSQL-----SD 732

  Fly   524 NFANSFFGLMMKKT----------NQLLAGFVEDSTNVQCLPE--EDFATVNTYLQFVNVYVRWL 576
            ..:..|:.:::|:.          |.|:...:::.|:...:.:  :|:..::            |
Yeast   733 PVSKEFWMIIVKRAFPWDTMVTFMNVLIVYLLDNQTSNSIIGDLCDDYDKLS------------L 785

  Fly   577 SISLDVWEPVRSEE-HSFIDCWAELTILFEYIECLMGKHKLEKNEILLDEDVALRGFTPLGRETL 640
            |..|:::.  ..|| ...:.||.  |:.|:.| |....|.:...:..  :::.::.:..|...|.
Yeast   786 SELLELFN--EGEELPEILGCWG--TLWFDTI-CEKNTHSISSEDNF--QEIGIKDYMALDSPTD 843

  Fly   641 SMDYLKRGSERLQFFERV-RRIGQFQEYYVQHQEAL---QQPL--ESSFTVEHLNAAMKNALENY 699
            .:.:.::.....:|::|. |.|..|:|........:   ..||  .|||...::..::...||
Yeast   844 GIIFDEKDENGEKFWKRACRTIFLFRELSRSFPIGVIIRNDPLIYRSSFQNTNILGSLVFKLE-- 906

  Fly   700 DAESCGLAANVSKIQCKAVEELLCRSTDSDVSELCKLKKELEAKARVKQICTSKLEDILKFVDTK 764
              ..|.:..|:..:  .|:|.::      |:|| .:.:...:..| |.::..::.::|..:|.
Yeast   907 --PLCNIHNNIPVL--GALESII------DISE-ARSENNTDLHA-VPELSVNEGDNIFHYVG-- 957

  Fly   765 IYIEVRPRY------------------LLPDTN------------------CFIDCLE------D 787
             |.::|..|                  .:|::|                  .|::|::      |
Yeast   958 -YKKLRADYTCFDKNGEFLSASLYTTWYVPNSNNTNIEDNINYNSEKENEGLFLECIKSDYPEID 1021

  Fly   788 FEKL-----STEFKRYT-------------LIIPLTVVKELDGLSKGVKLDSYRSSKQ---TQRI 831
            |:..     :|.:.|::             ..|.||..:||..|.|....:...::.:   |.|.
Yeast  1022 FKTTYFVFDATSWLRHSARIFKLAQNRLLRFAICLTTFQELRFLRKSKDENVMEAATRGIITIRQ 1086

  Fly   832 HHFD----------EVSSRAKKSLEFIKSAKGNVKCATTKGSFVNASVFALVEE----------- 875
            .:::          .|::..:::|||    :..:...|....||..||....|:
Yeast  1087 LYYENKVLPLRFTGNVATHIEENLEF----EEQITWRTHVDEFVIESVMKAQEKLESASEPRLSP 1147

  Fly   876 ---EY---LSNDD-----------KILATAMAVSKTAKT-EQ---CTD 902
               .|   :|:||           |.|:|....|...|. ||   |||
Yeast  1148 RRFNYVVLISDDDAMKKKAEEKEIKTLSTRFVFSLCTKLGEQRHLCTD 1195

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smg6	NP_651321.1	EST1	219..298	CDD:463062	21/118 (18%)
		EST1_DNA_bind	310..639	CDD:431239	69/461 (15%)
		PIN_Smg6-like	767..941	CDD:350233	43/241 (18%)
ESL2	NP_013022.1	PIN_SF	1027..1159	CDD:475124	23/135 (17%)