Protein Alignment Smg6 and EST1

DIOPT Version :10

• Sequence 1: NP_651321.1
• Gene: Smg6 / 42994
• FlyBaseID: FBgn0039260
• Length: 948
• Species: Drosophila melanogaster
• Sequence 2: NP_013334.1
• Gene: EST1 / 850934
• SGDID: S000004223
• Length: 699
• Species: Saccharomyces cerevisiae
• Alignment Length: 819
• Identity: 148/819 - (18%)
• Similarity: 268/819 - (32%)
• Gaps: 310/819 - (37%)

  Fly   226 KLLFYNVRNYLKRQQT-----------------DQAHTHTL-----LLIEQAIKFYRMLYDKLMA 268
            :|.|.|.|.:|.:..|                 |..|..:.     ||::|...::...|:::.
Yeast    12 RLFFKNARAHLDKHLTSRLTCDENAYITFRCFLDGIHRKSTRFLEELLLKQENMYHNNNYERIN- 75

  Fly   269 KCVASSRCDSALKVVAQRLLICLGDLTRYRVNHVKATDYMEAARYYQRAQELVPGNGAPFNQLAV 333
                    ||.:.:|.:.|.:.:.:.|.....|.    :.:..|...|            .:..|
Yeast    76 --------DSVIPLVLKLLWLQIHEPTLQWFEHW----FHDIMRLSNR------------RKFRV 116

  Fly   334 ISIYHHK--RFDAV---YYYVRSLLTSNSIQSAKESLLDLFDEIRRKYEETEM-------KQSPM 386
            ..|:..|  :|..:   |||                  |:.:.:..||:...:       |.:.|
Yeast   117 FRIFQKKMIQFFKITHRYYY------------------DIIEHLCAKYDMNSVISNALFAKLNLM 163

  Fly   387 HYAAG-SKNQK-----SKQMRKEVWIYPDGIRR--------------LHRTDDKGNKAKGNKATM 431
            .|..| |.::|     |..:...:.|   .::|              |::..:|....:|.:.::
Yeast   164 QYTDGLSTHEKIILNTSNPLTFSIVI---SLQRCVINLGSTHFYKTLLNKPSNKPKSVEGFEKSI 225

  Fly   432 AEVNRYEEMPPEEILPRIVSLYLYLLG-------KLYTGTDVDSLY--PLLRKLQIQIGVALRHE 487
            ..:|             |.||||..:|       |:|..|...|||  .|:|...::|.......
Yeast   226 RYLN-------------IASLYLPAVGDTYFQRAKIYLITGKFSLYFFELVRGALVRIPSKCALN 277

  Fly   488 NLLSRSKLLKIVALNLFVVEHNKPKTSRREMRYHSFNFANSFFGLMMKKTNQLLAGFVEDSTNVQ 552
            ||..            |::..:.|:..|                 :|||...|::..::.
Yeast   278 NLKD------------FILTPDFPERRR-----------------LMKKLAILVSKDLKG----- 308

  Fly   553 CLPEEDFATVNTYLQFVNVYVRWLSISLDVWEPVRSEEHSFIDCW---------------AELTI 602
               |:.|......|||:::...  ::....|...|:.     :||               ..:.:
Yeast   309 ---EKSFFEGQIVLQFLSIVEH--TLVPQSWNASRAS-----NCWLLKEHLQMAALKYHSGNINV 363

  Fly   603 LFEYIECLMG---------KHKLEKNEI-----------LLD---------EDVALR-------- 630
            :.|.:...||         |.|.:||::           .||         .||.::
Yeast   364 ILENLAATMGSFDLMFTTRKSKEQKNKLKYADLSERQVFFLDLSFDFIANIIDVVIKPSWQKNME 428

  Fly   631 GFTPLGRETLSMDYLKRGSERLQFFERVRRIGQFQEYYVQHQEALQQPLESSFTV-EH------- 687
            .|..|....|.|.::|.....||:..|.|:.  ...:.:...:.:..||..|..: .|
Yeast   429 DFRYLAIIRLLMCWIKSYRSILQYTHRHRKF--CTSFALLLNDLINSPLNCSGNIYSHRPKRSYL 491

  Fly   688 ------------LNAAMKNALENYDAESCGLAANVSKIQCKAVEELLCRSTDSDVSELCKLKKEL 740
                        :|.|:.:..::|..:|..:..|:  |.|..:.::|      ...|.|.|
Yeast   492 FREDIIFREFSCINFALTDFNDDYVYDSPDMINNI--IGCPTLTKVL------SPKEECVL---- 544

  Fly   741 EAKARVKQICTSKLEDILKFVDTKIYIEVRPRYLLPDTNCFIDCLEDFEKLSTEFKRYTLIIPLT 805
                |::.|..|.    :||::..            ||....:        ::::| :.||.|..
Yeast   545 ----RIRSIIFSG----MKFLEKN------------DTGVIWN--------ASKYK-FDLISPNI 580

  Fly   806 VVKELDGLSKGVKLDSYRSSK-----QTQRIHHFDEVSSR---AKKSLEFIKSAKGNVKCATTKG 862
            .:|....||:       .|||     |.:|:     ||||   ||:..:..|.| |.:.....:.
Yeast   581 KIKRQIALSE-------ISSKINVKTQQERV-----VSSRKVEAKRDEQQRKRA-GKIAVTELEK 632

  Fly   863 SFVNA----SVFALVEEEYLSNDDKILATAMAVSKTAKT 897
            .|.|.    .:..|.|::.:|::    ....|.|:..||
Yeast   633 QFANVRRTKKLSPLPEKDGVSSE----LVKHAASRGRKT 667

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smg6	NP_651321.1	EST1	219..298	CDD:463062	17/93 (18%)
		EST1_DNA_bind	310..639	CDD:431239	71/421 (17%)
		PIN_Smg6-like	767..941	CDD:350233	32/143 (22%)
EST1	NP_013334.1	EST1	81..209	CDD:463062	25/164 (15%)
		EST1_DNA_bind	224..508	CDD:431239	59/342 (17%)