Protein Alignment nAChRalpha1 and Chrna9

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_075219.2
• Gene: Chrna9 / 65024
• RGDID: 621534
• Length: 479
• Species: Rattus norvegicus
• Alignment Length: 543
• Identity: 195/543 - (35%)
• Similarity: 281/543 - (51%)
• Gaps: 92/543 - (16%)

  Fly    10 FIALHFATGGL-----ANPD-AKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKN 68
            |..::||..|:     ||.. |::|:.||..:|:..:|||.:....|.|.:.:.|||:.|::.:|
  Rat    10 FCWMYFAASGIRAVETANGKYAQKLFSDLFEDYSSALRPVEDTDAVLNVTLQVTLSQIKDMDERN 74

  Fly    69 QIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHH 133
            ||:|..:|:.|.|:|..|.|:.|.|..:|::.:||:.:|.|||||||.||......:.|..:|.:
  Rat    75 QILTAYLWIRQTWHDAYLTWDRDQYDRLDSIRIPSDLVWRPDIVLYNKADDESSEPVNTNVVLRY 139

  Fly   134 TGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQ 198
            .|.:.|..|||.||.|.:||.|||||.|.|.:.||||||:|..||:.:   ..||.      ||.
  Rat   140 DGLITWDSPAITKSSCVVDVTYFPFDSQQCNLTFGSWTYNGNQVDIFN---ALDSG------DLS 195

  Fly   199 DYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFY 263
            |:...|||::..:|||:|...|.||.|||.|:.|.|.|:|::.||.|||:||||.||||:.|.||
  Rat   196 DFIEDVEWEVHGMPAVKNVISYGCCSEPYPDVTFTLLLKRRSSFYIVNLLIPCVLISFLAPLSFY 260

  Fly   264 LPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVN 328
            ||:.||||:||.::|||::|||.|::|||: |.|..|||:|||.:.||.|:|.|..:||.|:|::
  Rat   261 LPAASGEKVSLGVTILLAMTVFQLMVAEIM-PASENVPLIGKYYIATMALITASTALTIMVMNIH 324

  Fly   329 F----RSPVTHRMAPWVQRLFIQILPKLL--------CIERPKKEEPEEDQPPEVLTDVYHLPPD 381
            |    ..||.|    |.:.:.::.:.::|        |:      .|...|.||.:|.||...|:
  Rat   325 FCGAEARPVPH----WAKVVILKYMSRILFVYDVGESCL------SPRHSQEPEQVTKVYSKLPE 379

  Fly   382 VDKFVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLN 446
            .:                  |..|...||:....:...               ..:||    |..
  Rat   380 SN------------------LKTSRNKDLSRKKEVRKL---------------LKNDL----GYQ 407

  Fly   447 GDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVL 511
            |.|                 |.....|....|...:...:||:.:|:.....|...:||.||.|:
  Rat   408 GGI-----------------PQNTDSYCARYEALTKNIEYIAKCLKDHKATNSKGSEWKKVAKVI 455

  Fly   512 DRMFLWIFAIACVVGTALIILQA 534
            ||.|:|||.....|.|.|||.:|
  Rat   456 DRFFMWIFFAMVFVMTVLIIARA 478

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	90/218 (41%)
		LIC	27..530	CDD:455710	184/514 (36%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
Chrna9	NP_075219.2	LIC	8..474	CDD:455710	191/537 (36%)
		LGIC_ECD_nAChR_A9	31..237	CDD:349823	90/214 (42%)
		Cys-loop	155..169	CDD:349823	9/13 (69%)