Protein Alignment nAChRalpha1 and chrna9a

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: XP_021326868.1
• Gene: chrna9a / 568807
• ZFINID: ZDB-GENE-090312-63
• Length: 580
• Species: Danio rerio
• Alignment Length: 601
• Identity: 200/601 - (33%)
• Similarity: 305/601 - (50%)
• Gaps: 101/601 - (16%)

  Fly     3 SVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLK 67
            |:|..|||.|        ....|::|.:||:..|:..:|||.:....|.|.:.:.|||:.|::.:
Zfish    11 SLLLHAVFSA--------QGRFAQKLLNDLMEGYSNALRPVEDTDKALNVTLQITLSQIKDMDER 67

  Fly    68 NQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNAD-----GNYEVTIMT 127
            ||::||.:||.|.|:|..|.|:.::|.|::.:.:||..:|.||||||||||     |..:    |
Zfish    68 NQVLTTYLWVRQIWHDAYLSWDKEEYDGLEVIRIPSNLVWRPDIVLYNNADEEDSSGPPD----T 128

  Fly   128 KAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIE 192
            ..:|.:.|::.|..|||.||.|::||.|||||.|.|.:.||||||:|..||:        :..:|
Zfish   129 NVVLRYNGEITWDSPAITKSSCKVDVSYFPFDSQECNLTFGSWTYNGNQVDI--------AMGME 185

  Fly   193 VGIDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFL 257
            .| ||.|:..:|||:...:|||:|...|.||.:||.||.:.:.|:|::.||..||::||..||||
Zfish   186 SG-DLSDFVDNVEWECHGMPAVKNVIMYGCCSDPYPDITYTVLLKRRSSFYIFNLLLPCFLISFL 249

  Fly   258 SVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTI 322
            :.|.||||:|||||:||.:::||:||||.|::||.:|| |.:|||:|||.:.||.::|.|..:||
Zfish   250 APLGFYLPADSGEKVSLGVTVLLALTVFQLMVAESMPP-SESVPLIGKYYIATMTMITASTSLTI 313

  Fly   323 AVLNVNF----RSPVTHRMAPWVQRLFIQILPKLLCIERPKKE--EPEEDQPP--------EVLT 373
            .::|::|    ..||.|    |.:.|.|..:.|:..:....:.  .||.|:.|        .:..
Zfish   314 FIMNIHFCGAEAKPVPH----WAKVLIIDYMSKIFFVYEVGENCTTPESDRGPFFSEDPLASLER 374

  Fly   374 DVY---------HLPPDV-DKFVNY---DSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPP 425
            |.|         ||...: .:|..|   |.:..:|.:...:....||.:.......|::   .|.
Zfish   375 DGYFDKGFYEDCHLDERIRSQFNGYSHRDHRHGNGYHHKNSSYRQHRHEQQRRTRSSSN---SPV 436

  Fly   426 LPSS----------------LPLPG----ADDDLFSPSGLNG---DISPGCCPAAAAAAAADLSP 467
            ..||                |||..    .|.::..|..:||   |...|.............|.
Zfish   437 RQSSHHPKYTHFIGRDGSEKLPLTSQEKLKDSEISIPEKINGYTYDQGNGYLNGVGYLHDNGYSK 501

  Fly   468 TFEKPYAREMEKTIEGS--------------RFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWI 518
            ||.   |....||..|:              .:||...:.:...::...:||.||.|:||.|:|:
Zfish   502 TFG---ADSYNKTTTGTGCVCGKHQKLVRNIEYIANCFREQRGHQAKGAEWKKVAKVMDRFFMWV 563

  Fly   519 FAIACVVGTALIILQA 534
            |.|...:.:.|::.:|
Zfish   564 FFIMVFLMSILVLAKA 579

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	90/222 (41%)
		LIC	27..530	CDD:455710	191/571 (33%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
chrna9a	XP_021326868.1	LGIC_ECD_nAChR_A9	25..232	CDD:349823	90/219 (41%)
		Cys-loop	150..164	CDD:349823	9/13 (69%)
		LGIC_TM_cation	233..>320	CDD:349853	47/87 (54%)
		TM1 helix	233..257	CDD:349853	13/23 (57%)
		TM2 helix	265..286	CDD:349853	11/20 (55%)
		TM3 helix	296..318	CDD:349853	8/21 (38%)