Protein Alignment nAChRalpha1 and chrne

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_001314812.1
• Gene: chrne / 445315
• ZFINID: ZDB-GENE-040808-32
• Length: 517
• Species: Danio rerio
• Alignment Length: 540
• Identity: 170/540 - (31%)
• Similarity: 278/540 - (51%)
• Gaps: 62/540 - (11%)

  Fly     9 VFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTT 73
            |.|||......:...:..:|..|...||::.|||..:..:::.|::.|.|:.||.:|.|.:.:||
Zfish    13 VAIALLATVLQVTGNEESQLIADKFKNYSKNIRPARHADEKVKVQVKLTLTNLISLNEKEETLTT 77

  Fly    74 NVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVV 138
            |||:|.:|:||:|.||..:|..::.:.||...:|||||||.||.||.::|......::...|.:.
Zfish    78 NVWIEIQWHDYRLAWNTSEYHDIEQIRVPYYTVWLPDIVLENNIDGKFDVAYYANVLISSDGSMY 142

  Fly   139 WKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDN---IE-VGIDLQD 199
            |.|||||:|.|.|::.|||||.|.|.:.|.|.||....:|:    ..|:.:|   || |.||.:.
Zfish   143 WLPPAIYRSTCAIEITYFPFDWQNCTLVFRSQTYSANEIDI----VLAEDENGKPIEWVDIDPEA 203

  Fly   200 YYISVEWDIMRVPA--VRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVF 262
            :..:.||.|...||  :.|:: ||..:..|.::.:|:.::||.|||.:|:|:||..||.|.||.:
Zfish   204 FTENGEWAIKHRPARKLTNQR-YSPDDLEYQEVYYNIIIQRKPLFYVINIILPCSLISSLVVLAY 267

  Fly   263 YLPSDS-GEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLN 326
            :||:.: |:|:::.||:||:.|||.:|:::.||.|||:|||:||||:|.|.:.||.|...|.|||
Zfish   268 FLPAKAGGQKLTVSISVLLAQTVFLILISQKIPETSLSVPLIGKYLIFVMSMTTLIVTNCIIVLN 332

  Fly   327 VNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNYDSK 391
            .:.|||.||.|:..::.:|::::|:.|                       .:.|.||:
Zfish   333 YSLRSPSTHNMSQSIKHIFLEVVPRYL-----------------------GMAPFVDE------- 367

  Fly   392 RFSGDY-GIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGDISPGCCP 455
               ||. |:..:....|...........:...:|          ..:.:|........:......
Zfish   368 ---GDQGGVYEMRERRRSSFGLMQRAEEYVLKQP----------RSEMMFDKQRERHGLMRTIVD 419

  Fly   456 AAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFA 520
            ....::.|:|..:..|. |.|:::.::...|||:..|.::...|..|.|..:..::|::..|...
Zfish   420 EIDVSSTANLYKSLAKT-APEIKECVDACNFIAESTKQQNDTGSEMESWVLIGKMIDKVCFWAAI 483

  Fly   521 IACVVGTALIIL-----QAP 535
            ....:||..|.|     |||
Zfish   484 SLFSIGTIAIFLMGHYNQAP 503

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	82/223 (37%)
		LIC	27..530	CDD:455710	161/510 (32%)
		Cys-loop	149..163	CDD:349832	8/13 (62%)
chrne	NP_001314812.1	LGIC_ECD_nAChR_G	52..245	CDD:349830	74/197 (38%)
		Cys-loop	153..167	CDD:349830	8/13 (62%)
		Neur_chan_memb	252..494	CDD:460753	75/285 (26%)