Protein Alignment nAChRalpha1 and Chrnb3

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_598281.1
• Gene: Chrnb3 / 171131
• RGDID: 621544
• Length: 464
• Species: Rattus norvegicus
• Alignment Length: 551
• Identity: 229/551 - (41%)
• Similarity: 316/551 - (57%)
• Gaps: 113/551 - (20%)

  Fly     6 FAAVFIALH----------FATGGLA----NPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGL 56
            |..||:.|.          .||.||:    :.||  |...|...|.:.:|||.|:||.:.|..||
  Rat     4 FLRVFLVLSATLSGSWVTLTATAGLSSVAEHEDA--LLRHLFQGYQKWVRPVLNSSDIIKVYFGL 66

  Fly    57 RLSQLIDVNLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNY 121
            ::|||:||:.|||:||||||::|||.|.||:|||::|||::::.||||.:|||||||:.||||.:
  Rat    67 KISQLVDVDEKNQLMTTNVWLKQEWTDQKLRWNPEEYGGINSIKVPSESLWLPDIVLFENADGRF 131

  Fly   122 EVTIMTKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTA 186
            |.::|||||:..:|.|.|.|||.|||.|.:||.:||||.|.|.||||||||||.||||..:.:
  Rat   132 EGSLMTKAIVKSSGTVSWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINE-- 194

  Fly   187 DSDNIEVGIDLQDYYISVEWDIMRVPAV---RNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLI 248
                   .:|.:|::.:.||:|:....:   |.|.|||     |..:.::..|||..||||:.||
  Rat   195 -------NVDRKDFFDNGEWEILNAKGMKGNRREGFYS-----YPFVTYSFVLRRLPLFYTLFLI 247

  Fly   249 IPCVGISFLSVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMML 313
            |||:|:|||:|||||||||.|||:||..|:|:|||||.|::.||||.:|..:||:|:||||.|:.
  Rat   248 IPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIF 312

  Fly   314 VTLSVVVTIAVLNVNFRSPVT-HRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYH 377
            ||||::||:.|:||:.||..| |.|||||:|||:|.||:.||::.|
  Rat   313 VTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQRLPRWLCMKDP------------------- 358

  Fly   378 LPPDVDKFVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSP 442
                :|:|               :.|.....|.|..|.:|.         .....|.:|.
  Rat   359 ----MDRF---------------SFPDGKESDTAVRGKVSG---------KRKQTPASDG----- 390

  Fly   443 SGLNGDISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYV 507
                                       |:.....:||..|..|:|::|||.:.....|.:|||:|
  Rat   391 ---------------------------ERVLVAFLEKASESIRYISRHVKKEHFISQVVQDWKFV 428

  Fly   508 AMVLDRMFLWIFAIACVVGTALIILQAPSLY 538
            |.||||:|||:|.||.|:|:.||.:.|..::
  Rat   429 AQVLDRIFLWLFLIASVLGSILIFIPALKMW 459

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	107/220 (49%)
		LIC	27..530	CDD:455710	216/506 (43%)
		Cys-loop	149..163	CDD:349832	8/13 (62%)
Chrnb3	NP_598281.1	LIC	29..455	CDD:455710	220/520 (42%)