Protein Alignment nAChRalpha1 and nAChRalpha2

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: XP_311921.3
• Gene: nAChRalpha2 / 1272988
• VectorBaseID: AGAMI1_000572
• Length: 569
• Species: Anopheles gambiae
• Alignment Length: 585
• Identity: 324/585 - (55%)
• Similarity: 406/585 - (69%)
• Gaps: 67/585 - (11%)

  Fly     1 MGSVLFAAVFIALHFATGGL-ANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDV 64
            :|.||...|.       |.: |||||||||||||||||||||||.||:|.:.||:|||||||||:
Mosquito    18 LGIVLLCMVI-------GSVWANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDL 75

  Fly    65 NLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKA 129
            |||:||:|||:|:|.||.|:|.||:|.:||||..|:|||||||||||||||||||.|.||.:|||
Mosquito    76 NLKDQILTTNIWLEHEWQDHKFKWDPAEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTLTKA 140

  Fly   130 ILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDN-IEV 193
            |||:||||:|.||||:||.|||||.|||||:||||||||||||||..:||:|..|..:||| :::
Mosquito   141 ILHYTGKVIWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGNQIDLKHKNQLNNSDNMVKI 205

  Fly   194 GIDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLS 258
            ||||::||.||||||:.|||.|:||:|.||.|||.||.||:|||||||||||||||||||||:||
Mosquito   206 GIDLREYYPSVEWDILGVPAERHEKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGISYLS 270

  Fly   259 VLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIA 323
            |||||||:||||||:||||||||.|:||||::||||.|||.:|||||||||||:||.||||:||.
Mosquito   271 VLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMILVGLSVVITII 335

  Fly   324 VLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFVNY 388
            :||:::|.|.||:|||||::.||..|||||.:..|.          ::|.::      ....:||
Mosquito   336 ILNIHYRKPSTHKMAPWVRKFFILRLPKLLLMRVPN----------DLLKEL------AANKINY 384

  Fly   389 DSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLP--------LPGADDDLFSP--- 442
            ..|.....:|               ..::||...:|....|.|        .||..:.|.|.
Mosquito   385 GIKISKSKFG---------------AALAAHSQMQPNSGGSSPDSIRHMQGRPGGCNGLHSTTAT 434

  Fly   443 ---SGLNGDISPGC--------CPAAAAA---AAADLSPTFEKPYAREMEKTIEGSRFIAQHVKN 493
               |||.|.:..|.        .|:..:.   :.:|::|  .|.|..|:||.|....||..|::.
Mosquito   435 NRFSGLVGALGGGLSGLGGYNGLPSVMSGLDESLSDVAP--RKKYPFELEKAIHNVMFIQHHMQR 497

  Fly   494 KDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTALIILQAPSLYDQSQPIDILYSKIAKKKFEL 558
            :|:|.:.::||.:|||||||:|||:|.||.:.||..|:.:||||||.::||||.||.||:|.|.|
Mosquito   498 QDEFNAEDQDWGFVAMVLDRLFLWLFTIASLFGTFAILCEAPSLYDDTKPIDIEYSIIAQKLFNL 562

  Fly   559  558
            Mosquito   563  562

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	160/218 (73%)
		LIC	27..530	CDD:455710	295/528 (56%)
		Cys-loop	149..163	CDD:349832	11/13 (85%)
nAChRalpha2	XP_311921.3	LIC	15..535	CDD:455710	306/556 (55%)
		LGIC_ECD_nAChR_proto_alpha-like	35..254	CDD:349832	160/218 (73%)
		Cys-loop	160..174	CDD:349832	11/13 (85%)