Protein Alignment nAChRalpha1 and Chrne

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: XP_006532069.1
• Gene: Chrne / 11448
• MGIID: 87894
• Length: 494
• Species: Mus musculus
• Alignment Length: 555
• Identity: 177/555 - (31%)
• Similarity: 270/555 - (48%)
• Gaps: 87/555 - (15%)

  Fly     1 MGSVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVN 65
            |...|..|:.:...|......|.:.. ||..|..||:...|||....|.:|:.:.:.|:.||.:|
Mouse     1 MAGALLGALLLLTLFGRSQGKNEELS-LYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLN 64

  Fly    66 LKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAI 130
            .|.:.:||:||:..:|:||:|.::.||:.||..|.|||||:|||:|||.||.||.:.|...:..:
Mouse    65 EKEETLTTSVWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVL 129

  Fly   131 LHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNI--EV 193
            ::..|.|.|.|||||:|.|.::|.|||||.|.|.:.|.|.||:...|:.  :....|..|.  ::
Mouse   130 VYEGGYVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEF--IFAVDDDGNTINKI 192

  Fly   194 GIDLQDYYISVEWDIMRVPAVRNEKFYSCCEEP-YLDIVFNLTLRRKTLFYTVNLIIPCVGISFL 257
            .||...:..:.||.|...|.:.........|.| ..|:::.|.:|||.|||.:|:|:|||.||.|
Mouse   193 DIDTAAFTENGEWAIDYCPGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGL 257

  Fly   258 SVLVFYLPSDS-GEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVT 321
            .:|.::||:.: |:|.::.|::||:.|||..|:|:.||.|||:|||||:||:|.|::.||.|:..
Mouse   258 VLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNC 322

  Fly   322 IAVLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLPPDVDKFV 386
            :.||||:.|:|.||..:|.::::.:::||:||. ..|..|:|....|
Mouse   323 VIVLNVSLRTPTTHATSPRLRQILLELLPRLLG-SSPPPEDPRTASP------------------ 368

  Fly   387 NYDSKRFSGDYGIPALPASH------RFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGL 445
                .|.:...|| .|.|..      |.:|...|....|
Mouse   369 ----ARRASSVGI-LLRAEELILKKPRSELVFEGQRHRH-------------------------- 402

  Fly   446 NGDISPGCCPAAAAA---AAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYV 507
                  |...|||..   .||          |.|:...::...|:|:..::::.......||..:
Mouse   403 ------GTWTAAALCQNLGAA----------APEIRCCVDAVNFVAESTRDQEATGEELSDWVRM 451

  Fly   508 AMVLDRMFLWIFAIACVVGTALIIL-----QAPSL 537
            ...||.:..|...:...||:.||.|     |.|.|
Mouse   452 GKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDL 486

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	82/220 (37%)
		LIC	27..530	CDD:455710	166/515 (32%)
		Cys-loop	149..163	CDD:349832	8/13 (62%)
Chrne	XP_006532069.1	LGIC_ECD_nAChR_E	49..239	CDD:349831	71/191 (37%)
		Cys-loop	148..162	CDD:349831	8/13 (62%)
		Neur_chan_memb	247..475	CDD:460753	81/293 (28%)