Protein Alignment nAChRalpha1 and Chrna4

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_056545.3
• Gene: Chrna4 / 11438
• MGIID: 87888
• Length: 629
• Species: Mus musculus
• Alignment Length: 619
• Identity: 260/619 - (42%)
• Similarity: 344/619 - (55%)
• Gaps: 105/619 - (16%)

  Fly     1 MGSVLFAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVN 65
            :|:.|..|   :.|..|...|.   :||...|.|.||:..|||.|.||.:.|:.||.::|||||:
Mouse    21 LGTGLLPA---SSHIETRAHAE---ERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVD 79

  Fly    66 LKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAI 130
            .|||:|||||||:|||:||||:|:|.||..|.::.:|||.||.|||||||||||::.||.:|||.
Mouse    80 EKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAH 144

  Fly   131 LHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGI 195
            |.:.|:|.|.|||||||.|.|||.:||||:|.|.|||||||||...:||..:...         :
Mouse   145 LFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSR---------V 200

  Fly   196 DLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVL 260
            |..|::.|.||.|:......|.:.|.||.|.|.||.:...:||..||||:||||||:.||.|:||
Mouse   201 DQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVL 265

  Fly   261 VFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVL 325
            ||||||:.|||::||||:|||||||.||:.||||.|||.:||:|:||||||:.||||:|:|:.||
Mouse   266 VFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVL 330

  Fly   326 NVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPK--KEE------------------PEEDQPPE 370
            ||:.|||.||.|..||:|:|:.|:|:||.::||.  |:.                  ||.:..|.
Mouse   331 NVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPEPESEPG 395

  Fly   371 VLTDV----------------------------YHLPPD-----VDK---FVNYDSKRFSGDYGI 399
            :|.|:                            .|..||     |:|   ..:..|.......|.
Mouse   396 ILGDICNQGLSPAPTFCNRMDTAVETQPTCRSPSHKVPDLKTSEVEKASPCPSPGSCHPPNSSGA 460

  Fly   400 PAL-----------PASHRFDLAAAGGISA------HCFAE---PPLPSSLPLPGADDDLFSPSG 444
            |.|           |:|..   ||.|.|..      :|.::   ..|..|.|..........||.
Mouse   461 PVLIKARSLSVQHVPSSQE---AAEGSIRCRSRSIQYCVSQDGAASLTESKPTGSPASLKTRPSQ 522

  Fly   445 L--NGDISPGCC---------PAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFE 498
            |  :...||..|         |.....|....:|....|.:..:.:.:||.::||.|:|.:|...
Mouse   523 LPVSDQTSPCKCTCKEPSPVSPITVLKAGGTKAPPQHLPLSPALTRAVEGVQYIADHLKAEDTDF 587

  Fly   499 SVEEDWKYVAMVLDRMFLWIFAIACVVGTALIIL 532
            ||:|||||||||:||:|||:|.|.|::||..:.|
Mouse   588 SVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFL 621

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	111/217 (51%)
		LIC	27..530	CDD:455710	253/589 (43%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
Chrna4	NP_056545.3	LGIC_ECD	39..245	CDD:475126	111/217 (51%)
		Neur_chan_memb	252..620	CDD:460753	137/370 (37%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	420..459		6/38 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	503..529		6/25 (24%)