Protein Alignment nAChRalpha1 and Chrna3

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: NP_660111.3
• Gene: Chrna3 / 110834
• MGIID: 87887
• Length: 499
• Species: Mus musculus
• Alignment Length: 540
• Identity: 239/540 - (44%)
• Similarity: 340/540 - (62%)
• Gaps: 56/540 - (10%)

  Fly     1 MGSVL----FAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQL 61
            ||.||    .:.:.:.|.......|:....||:..|..:||.:||||.|.|..:.::..:.:|||
Mouse     1 MGVVLPPPPLSMLMLVLMLLPVASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQL 65

  Fly    62 IDVNLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIM 126
            :.|:..||||.||:|::|.||||||||.|.||.||:.:.||:|.||.|||||||||||:::|...
Mouse    66 VKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDK 130

  Fly   127 TKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNI 191
            |||:|.:||:|.|.||||:||.|:|||.|||||.|.|.||||||:||...:||           :
Mouse   131 TKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDL-----------V 184

  Fly   192 EVG--IDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGI 254
            .:|  ::|:||:.|.||.|::.|..::|..|:||||.|.||.::|.:||..||||:||||||:.|
Mouse   185 LIGSSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLI 249

  Fly   255 SFLSVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVV 319
            |||:|||||||||.|||::||||:|||||||.|::.|.||.|||.:||:|:||||||:.||||:|
Mouse   250 SFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIV 314

  Fly   320 VTIAVLNVNFRSPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDVYHLP-PDVD 383
            :|:.||||::|:|.||.|..||:.:|:.:||:::.:.||  ...|||.|.  ..:.|... .:::
Mouse   315 ITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRP--TSTEEDAPK--TRNFYGAELSNLN 375

  Fly   384 KFVNYDSKRFSGDYGIPALPASHRFDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFSPSGLNGD 448
            .|...|||  |...|.|           ...|...:|.......|:          || :.|...
Mouse   376 CFSRADSK--SCKEGYP-----------CQDGTCGYCHHRRVKISN----------FS-ANLTRS 416

  Fly   449 ISPGCCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDR 513
            .|.....|..:.:|  |||        |:::.|:..::||:::|.::..:.:::||||||||:||
Mouse   417 SSSESVDAVLSLSA--LSP--------EIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDR 471

  Fly   514 MFLWIFAIACVVGTALIILQ 533
            :|||:|.:.|::|||.:.||
Mouse   472 IFLWVFILVCILGTAGLFLQ 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	109/219 (50%)
		LIC	27..530	CDD:455710	231/505 (46%)
		Cys-loop	149..163	CDD:349832	10/13 (77%)
Chrna3	NP_660111.3	LIC	24..490	CDD:455710	232/514 (45%)
		LGIC_ECD_nAChR_A3	29..235	CDD:349817	109/216 (50%)
		Cys-loop	153..167	CDD:349817	10/13 (77%)