Protein Alignment nAChRalpha1 and chrna4

DIOPT Version :10

• Sequence 1: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Sequence 2: XP_031749826.1
• Gene: chrna4 / 100135106
• XenbaseID: XB-GENE-5994598
• Length: 762
• Species: Xenopus tropicalis
• Alignment Length: 619
• Identity: 250/619 - (40%)
• Similarity: 343/619 - (55%)
• Gaps: 112/619 - (18%)

  Fly     6 FAAVFIALHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQI 70
            |:..|  .|..|...|.   :||...|.|.||:..|||.|.||.:.|:.||.::|||||:.|||:
 Frog   156 FSTAF--CHIETRAHAE---ERLLKGLFSGYNKWSRPVANISDAVMVRFGLSIAQLIDVDEKNQM 215

  Fly    71 MTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTG 135
            |||||||:|||:||||:|:|.:|..|.::.:|||.||.|||||||||||::.||.:|||.|.|.|
 Frog   216 MTTNVWVKQEWHDYKLRWDPLEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDG 280

  Fly   136 KVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDY 200
            ::.|.|||||||.|.|||.:||||:|.|.|||||||||...:||         .::...:|..||
 Frog   281 RIKWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDRAKIDL---------ISMHSHVDQLDY 336

  Fly   201 YISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLP 265
            :.|.||.|:......|.|.|.||.|.|.||.::..:||..||||:||||||:.||.|:|||||||
 Frog   337 WESGEWVIVNAVGNYNIKKYECCTEIYSDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLP 401

  Fly   266 SDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFR 330
            |:.||||:||||:|||||||.||:.||||.|||.:||:|:||||||:.||||:::|:.||||:.|
 Frog   402 SECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHR 466

  Fly   331 SPVTHRMAPWVQRLFIQILPKLLCIERPKKEEPEE----------DQPPEVLTDVYHLPPDVDKF 385
            ||.||.|..||:|.|:.::|::|.::||.|:..::          :.||.:.::....|    .|
 Frog   467 SPRTHTMPAWVRRTFLDVVPRVLFMKRPAKDNCKKLIESLHARSFNPPPRLWSEAEIEP----AF 527

  Fly   386 VNYDSKRFSGDYGIPALPASHR---------FDLAAAGGISAHCFAEPPLPSSLPLPGADDDLFS 441
            .:..|...:.....|.....|:         |..:.:|..|..|  |....:|.|.|..:..|.|
 Frog   528 ASSSSASPTSQRPSPTSSCCHQLEQSPNAQVFCKSPSGQYSMLC--EESALTSCPSPKPETKLLS 590

  Fly   442 PSG-------------------------------------LNGDISPGCCPAAAAAAA------- 462
            .:.                                     |:.|.|.....|:::..|
 Frog   591 EAQPNLMPKVRSVSVQQMYTPKEANEGAVRCRSRSIQYCFLHEDFSQANGQASSSPVAQKGNGNE 655

  Fly   463 ----ADLSPTFEKPYARE-------------------------MEKTIEGSRFIAQHVKNKDKFE 498
                :..||...||.::|                         ::..|||..:||.|::.:|...
 Frog   656 PQPSSHTSPCTCKPKSQEKPVDPSQGAKTVNAKEHHVLLMSPSLKLAIEGVHYIADHLRAEDADF 720

  Fly   499 SVEEDWKYVAMVLDRMFLWIFAIACVVGTALIIL 532
            ||::||||||||:||:|||:|.|.|::||..:.|
 Frog   721 SVKQDWKYVAMVIDRIFLWMFIIVCLLGTVGLFL 754

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	112/217 (52%)
		LIC	27..530	CDD:455710	244/594 (41%)
		Cys-loop	149..163	CDD:349832	9/13 (69%)
chrna4	XP_031749826.1	LGIC_ECD	170..376	CDD:475126	112/217 (52%)
		Neur_chan_memb	383..753	CDD:460753	127/375 (34%)