DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ask1 and Map3k5

DIOPT Version :9

Sequence 1:	NP_477089.3	Gene:	Ask1 / 42366	FlyBaseID:	FBgn0014006	Length:	1363	Species:	Drosophila melanogaster
Sequence 2:	NP_001264623.1	Gene:	Map3k5 / 365057	RGDID:	1306565	Length:	1381	Species:	Rattus norvegicus

Alignment Length:	1347	Identity:	559/1347 - (41%)
Similarity:	782/1347 - (58%)	Gaps:	155/1347 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 ALEEIKQAVQSAGANFQRVQFERLDFGETNVLETFYNADVAIIDLSILTQQRPLSYHYGVRESFG 85
            ||:.:::|.::.||..:.:.|.:||||||.||:.|||||:|::::|...:|..|.||.||||||.
  Rat   119 ALQSLREACEAVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFS 183

  Fly    86 MKENILTYNDIDSKQTLSLK-LSC-------ANYLFLSYKRNAETN-SCHLTSQPNSGNNSKEPN 141
            |..||:.|.|.:|....||| :.|       .||.|:.|....... .|..:|.........:||
  Rat   184 MANNIILYCDTNSDSLQSLKEIICQKNTVCTGNYTFIPYMVTPHNKVYCCDSSFMKGLTELMQPN 248

  Fly   142 AE---GRV--PTLQWRLKRKLQDVEIQSKAHMREKFLSDMRTARDTYATNGAKLQSILHEMRKRL 201
            .|   |.:  |.:. |..:.|:..:..|..:.||..|:|:|.||:.|  .|.:|.:.|..:|:|:
  Rat   249 FELLLGPICLPLVD-RFVQLLKVAQASSSQYFRESILNDIRKARNLY--TGKELAAELARIRQRV 310

  Fly   202 DDPHVLSGEVVHSFMCSLRDVQDYDAMVRLVNDLKNIPNTRKYVETGNMSFLYAFALNRRNRKGD 266
            |:..||:.::|.:.:.|.||:||||::|:||..|:.:| |.......::.|.|||||||||..||
  Rat   311 DNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLP-TFDLASHHHVKFHYAFALNRRNLPGD 374

  Fly   267 REKALESSLKALEKKENHFPDMLCLCGRIYKDIFVESDYTDATSLAHAIKWYRQSFEVQPNEYAG 331
            |.|||:..:..::.:|....||.||.||||||:|::|::||..|..|...|::::||.:|...:|
  Rat   375 RAKALDIMIPMVQSEEQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSG 439

  Fly   332 INLATLLVIEGKEFTNTEELQHIGMTLNNLIGKKGSLSSLSEYWDVATFFEISVLANDYAKAIQA 396
            ||.|.||:..|.:|.::.||:.:|:.|::|:||||:|..|..||:|..|...||||||:.:.|||
  Rat   440 INYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHLRVIQA 504

  Fly   397 AECMFKLKPPNWYLKSTIGNISLIHRFRKKPEERQPPIEEQVFQFWMDFFLEATNTEEVKNSIRF 461
            :|.:|:||.|.|||||.:..|.:...|.|...| ||..::::..|||||.:|||.|:  ...:||
  Rat   505 SEKLFRLKTPAWYLKSIVETILIYKHFVKLTTE-QPTAKQELVDFWMDFLVEATKTD--VTVVRF 566

  Fly   462 PILILEPQKIYMPSYVTINMDADEKSIQIVNICLAHAKNACKKIHDFLFVASKIKSVSLYKRDDR 526
            |:|||||.|||.|||::||.:.:||:|.|.:: |...|   |.||::.|.||.::.||:.|.::|
  Rat   567 PVLILEPTKIYQPSYLSINNEVEEKTISIWHV-LPDDK---KGIHEWNFNASSVRGVSISKFEER 627

  Fly   527 CAYLYVHHNSDDFQIYFPSTERRQKFYDMILEMTADQVVFVNLSN-DDANIEYEYDYDEQNRKMV 590
            |.:|||.||||||||||.:....::|::|:..:|.::.......: :..::||:|::||...::|
  Rat   628 CCFLYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEEGDCEGDSLEYDYEHDENGDRVV 692

  Fly   591 LGKGTYGTVYAARDKQTQVRIAIKEVPEKNSQDVQPLHEEIKLHSQLRHRNIVRYLGSCSENGFF 655
            |||||||.|||.||...||||||||:||::|:..|||||||.||..|:|:|||:||||.|||||.
  Rat   693 LGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFI 757

  Fly   656 KIFMEQVPGGSLSDLLETKWGPLKDNESTMAFYSKQILEGLKYLHEQDIVHRDIKGDNVLVNTYS 720
            |||||||||||||.||.:|||||||||.|:.||:|||||||||||:..||||||||||||:||||
  Rat   758 KIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYS 822

  Fly   721 GVVKISDFGTSKRLARINPMTETFTGTLQYMAPEVIDQGVRGYGPAADIWSFGCTNVEMATGKPP 785
            ||:||||||||||||.|||.||||||||||||||:||:|.||||.||||||.|||.:||||||||
  Rat   823 GVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPP 887

  Fly   786 FIELGSAHAAMFKVGFYKKHPNIPEELSANAKNFILRCFAISVMDRPSASQLLEDPFLQ--DKPR 848
            |.|||...|||||||.:|.||.|||.:||.||.|||:||......|..|:.||.|.||:  .|.:
  Rat   888 FYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKK 952

  Fly   849 KVRPALP----INTEFGRSISVPADRFVHKTTPPLSYNTTCNTPTTPELDITHSSSVDIDELPSN 909
            |.:|.|.    ...|:.||||:|....|..|:....|.:.  :|.|              ||.::
  Rat   953 KTQPKLSGMWLHGEEYLRSISLPVPVLVEDTSSSSEYGSV--SPDT--------------ELKAD 1001

  Fly   910 QFMLE-RRNSHG----------FLLSPE------IEPSTPSLRTSISETSETDGFYRLKKDSQRR 957
            .|..: |..|.|          ||..|:      ..|.:|..:.|        ||:.|:|||:||
  Rat  1002 PFSFKTRAKSCGEKDGKGIRTLFLGIPDENFEDHSAPPSPEEKDS--------GFFMLRKDSERR 1058

  Fly   958 TTLSKVLALDESKICDIWLKKVDADQNSIAIRKSDLEVLIRGLRDYIMNENEKHLEATINELKQK 1022
            .||.::|..|:.|:....::.:........::...:..||..||:::.:.:.|.:..|::.||.:
  Rat  1059 ATLHRILTEDQDKVVRNLMESLAQGPEEPKLKWEHITTLISSLREFVRSTDRKIIATTLSRLKLE 1123

  Fly  1023 LNNDAVALDHLHLALYSFQDAVVCVLRYHCIKPHWMFALDNLVKRAVQAAVTIFSPEL------- 1080
            |:.|:..:..:.:.|:.|||||..|||.|.||||||||||:::::|||.|:||..|||
  Rat  1124 LDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLA 1188

  Fly  1081 -GANLAD-KDLSGNDDESLRTSLMQKGSTESQEKQSLEKVLPTSNTREGVGLTPAESGGDALSSS 1143
             .::.|| :||..:|:..           ||...|:..:..|.:..      ..|.||...|||:
  Rat  1189 SESDTADPEDLDVDDEHE-----------ESPSNQTARRPQPVAED------AVATSGVSTLSST 1236

  Fly  1144 --------------------MECARILRDIRDNQQKLQRQLLEQQRQSMRALHNISQELAHIYMG 1188
                                ||..|:|.::...:::|| .||.|      |:....||:.|:.:
  Rat  1237 VSHDSQNAHRSLNVQLGRMKMETNRLLEELVRKEKELQ-ALLHQ------AIEEKDQEIKHLRL- 1293

  Fly  1189 GRKRLRRTINGFNKKCHRMSTSVGGTSGVRPNIAGNPRRQLNKHHFGLHEVDEQLEQWLTQQEID 1253
                          |...:.|            .|.|...||..  |....|.:|..||.:...|
  Rat  1294 --------------KSQPIDT------------PGFPVCHLNAP--GTAIEDSELTGWLRENGAD 1330

  Fly  1254 EFSKTLILNEGFTFDDFIYNMEKLDLMRLGLRVGIEVRLWKLIIQER 1300
            |.:.:..|.|.:|..|.:|.:.:.||..|.||.|:...|||.||..|
  Rat  1331 EDTISRFLAEDYTLVDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFR 1377

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ask1	NP_477089.3	DUF4071	74..444	CDD:290020	150/383 (39%)
		STKc_ASK	576..843	CDD:270794	192/266 (72%)
		S_TKc	590..843	CDD:214567	187/252 (74%)
Map3k5	NP_001264623.1	DUF4071	172..551	CDD:290020	150/383 (39%)
		STKc_ASK	678..945	CDD:270794	192/266 (72%)
		S_TKc	692..945	CDD:214567	187/252 (74%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166351741
Domainoid	1	1.000	385	1.000	Domainoid score	I775
eggNOG	1	0.900	-	-		E1_KOG4279
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	908	1.000	Inparanoid score	I351
OMA	1	1.010	-	-		QHG45642
OrthoDB	1	1.010	-	-		D122434at33208
OrthoFinder	1	1.000	-	-		FOG0001687
OrthoInspector	1	1.000	-	-		otm44515
orthoMCL	1	0.900	-	-		OOG6_104013
Panther	1	1.100	-	-	O	PTHR11584
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1591
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	14	13.810