Protein Alignment Ire1 and RAD53

DIOPT Version :10

• Sequence 1: NP_001097839.1
• Gene: Ire1 / 42358
• FlyBaseID: FBgn0261984
• Length: 1074
• Species: Drosophila melanogaster
• Sequence 2: NP_015172.1
• Gene: RAD53 / 855950
• SGDID: S000006074
• Length: 821
• Species: Saccharomyces cerevisiae
• Alignment Length: 790
• Identity: 160/790 - (20%)
• Similarity: 269/790 - (34%)
• Gaps: 270/790 - (34%)

  Fly   359 INDVLQEHAGIMLGHYNMPNEGNGNLQLSPTTASSKVVQSLATIHNYNDGYGLLANNEKNAADIG 423
            |:.:..:|..|:||.       :|||.|:..:.:...:.......|.|.   ||:..::....:|
Yeast    81 ISRLSNKHFQILLGE-------DGNLLLNDISTNGTWLNGQKVEKNSNQ---LLSQGDEITVGVG 135

  Fly   424 VQTDPELVEIGIDQRTNGNTINRTKTIILQNSNKVQAFINEWFMEHPSGKVHQILIVIVLGMIAL 488
            |::|                       ||    .:..|||:.|.:
Yeast   136 VESD-----------------------IL----SLVIFINDKFKQ-------------------- 153

  Fly   489 FWYTCSTMRELQKQSENGSKTFAIAQNGSNGSTGSNGSNANAEDLVDLGNGQVRVGKISFS-TNE 552
                |....::.:...|...|..||..|...||.|:        :|....|..:    .|| .:|
Yeast   154 ----CLEQNKVDRIRSNLKNTSKIASPGLTSSTASS--------MVANKTGIFK----DFSIIDE 202

  Fly   553 VLGKGCEGTFVFKG-------TFEERFVAVKRLLPECFTFADREVALLRESDAHENVVRYFCTEQ 610
            |:|:|...| |.|.       ||..:.::.::::.. .....||:.:|::.: |..:||.....:
Yeast   203 VVGQGAFAT-VKKAIERTTGKTFAVKIISKRKVIGN-MDGVTRELEVLQKLN-HPRIVRLKGFYE 264

  Fly   611 DRQFRYIAVE-LCAATLQDYTEG------DRSLELQNHIDVWQVLSQAASGLSHLHSLDIVHRDI 668
            |.:..|:.:| :....|.|:...      |...|:..         |..:.:.::||:.|.|||:
Yeast   265 DTESYYMVMEFVSGGDLMDFVAAHGAVGEDAGREISR---------QILTAIKYIHSMGISHRDL 320

  Fly   669 KPQNVLISLPDAKGKVRVMISDFGLCKKLNFGKTSFSRRSGVTGTDGWIAPEMMRSQRTTTA--- 730
            ||.|:||...|   .|.|.|:||||.|..  |..||.:.  ..||..::|||::|.:.|:.:
Yeast   321 KPDNILIEQDD---PVLVKITDFGLAKVQ--GNGSFMKT--FCGTLAYVAPEVIRGKDTSVSPDE 378

  Fly   731 ----------VDIFSLGCVYYYVLSGGHHAFG----DNLKRQANILS-HEYNLAKLRPEDDSEDS 780
                      ||::|:||:.|.:|: ||..|.    |.|.:|....| ||..|...|..:::.|
Yeast   379 YEERNEYSSLVDMWSMGCLVYVILT-GHLPFSGSTQDQLYKQIGRGSYHEGPLKDFRISEEARD- 441

  Fly   781 RIILAEQLISDMIHKDPQSRPPARCIGNHPLFWDEPKMLSFLQDVSDRVEKLQFHAEPLKSLEKN 845
                   .|..::..||.:|..|....|||  |                    ....||.|
Yeast   442 -------FIDSLLQVDPNNRSTAAKALNHP--W--------------------IKMSPLGS---- 473

  Fly   846 GRIVVLDDWNVHLDPMITDDLRKYRGYMGASVRDLL---RALRNKKHHYHELTPAAQKMLGCIPH 907
                                 :.|..:...|:...|   :.|.|.....:|...|.:|:
Yeast   474 ---------------------QSYGDFSQISLSQSLSQQKLLENMDDAQYEFVKAQRKL------ 511

  Fly   908 EFTNYWVDRFPQLISHAYHAFSICSNEPIFKPYYSAGYLFTRPWYFDADDALFPMLMLDPKPLPK 972
            :......::..:........|.|.::.||         .:|:|...:|:.....:|
Yeast   512 QMEQQLQEQDQEDQDGKIQGFKIPAHAPI---------RYTQPKSIEAETREQKLL--------- 558

  Fly   973 IGSPKKTPSPASSQAQQLKQRKGLYNFRKPNDEL----PIPGVGLQRNLELDGQSLEP------- 1026
                      .|:..:.:|..|     :|.|...    |:|...:|.:||:. |.:.|
Yeast   559 ----------HSNNTENVKSSK-----KKGNGRFLTLKPLPDSIIQESLEIQ-QGVNPFFIGRSE 607

  Fly  1027 -------DGKRDVFANFKFRR--------YSKP-------------------GNNRNYNGGHKEA 1057
                   |.:......|.|::        |..|                   .|||...|.....
Yeast   608 DCNCKIEDNRLSRVHCFIFKKRHAVGKSMYESPAQGLDDIWYCHTGTNVSYLNNNRMIQGTKFLL 672

  Fly  1058 QDKEKY-VIW 1066
            ||.::. :||
Yeast   673 QDGDEIKIIW 682

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ire1	NP_001097839.1	Luminal_IRE1	53..321	CDD:188875
		STKc_IRE1	545..813	CDD:270884	82/300 (27%)
		RNase_Ire1	817..941	CDD:199217	15/126 (12%)
RAD53	NP_015172.1	FHA_RAD53-like_rpt1	35..148	CDD:438741	19/103 (18%)
		STKc_Rad53_Cds1	196..465	CDD:271000	82/298 (28%)
		FHA_RAD53-like_rpt2	577..695	CDD:438742	20/107 (19%)