Protein Alignment Ire1 and ern2

DIOPT Version :10

• Sequence 1: NP_001097839.1
• Gene: Ire1 / 42358
• FlyBaseID: FBgn0261984
• Length: 1074
• Species: Drosophila melanogaster
• Sequence 2: XP_001919350.4
• Gene: ern2 / 567021
• ZFINID: ZDB-GENE-090312-15
• Length: 931
• Species: Danio rerio
• Alignment Length: 975
• Identity: 389/975 - (39%)
• Similarity: 529/975 - (54%)
• Gaps: 166/975 - (17%)

  Fly    53 EALMVFSTLGGGLTAIDPVTSEIRWTIADDPPIVAEHQENVQVPHFLPDPRDGSIYQLG--QMGS 115
            |:|:..|||.|.|.|:...|.:|:||:.:||  :.:..|..|.|.|||||.|||:|.||  :...
Zfish    35 ESLLFVSTLDGSLHAVSKQTGDIKWTLKEDP--IIQVPEYFQEPGFLPDPNDGSLYVLGGKRKEG 97

  Fly   116 LKKLPYTIPQLVANAPCRSSDGILYSGKKSDTWYMVDPKTGRREKVMGFGDATVDGKEGEQIGWA 180
            |.|||:||.:||.::|||||||:||:|||.|||::|||:||.::       .::.....:.| ..
Zfish    98 LMKLPFTIQELVQSSPCRSSDGVLYTGKKQDTWFVVDPQTGEKQ-------TSLSTSSSDSI-CP 154

  Fly   181 TSRAIYLGRTQYTVMMYDSLAKNKDAKPWNITFYDYNAVSAPPELAKEYEY--IHLTTTTNGQIV 243
            ::..:|:|||:|.:.|||:  |.::.: ||.|:.||   |||....|:|||  .|..::.:|.:|
Zfish   155 SAPLLYIGRTEYMITMYDT--KTQELR-WNATYNDY---SAPLYDDKKYEYKMSHFASSGDGLVV 213

  Fly   244 TLDRKHGKFLWQRDLSSPVVAAFLLGPDGLLSVPFTTVSDEAYQAILEESKTGNVNTVK------ 302
            |:||..|:.||.:...|||...:|...|.|...|..||:.|..:.:...::....:|:|
Zfish   214 TVDRDSGEVLWMQKFDSPVAGFYLWSQDSLRRAPHLTVATETLRYLTFTAENTQSHTMKWTYQFT 278

  Fly   303 ---------LFQSLYVGEHQKGLYALPSLVDKNTPRISTAPPIKLLDGP------TGDQNSNQET 352
                     |..:||||:.:..|||..|||.:....:.....:..::||      .|:.....|.
Zfish   279 KESHSTKTQLVPTLYVGKTESHLYASASLVHQGVAIVPKGLTLARIEGPMTAGVTMGNGRPECEI 343

  Fly   353 DPRTIYINDVLQ--------EHAGIMLGHYNMPNEGNGNLQLSPTTASSKVVQSLATIHNYNDGY 409
            .|.|    ||..        ::..:::||:.:|       .|:.||......::|
Zfish   344 TPST----DVKYPPGSSSSLQNQWLLIGHHEIP-------PLAHTTMLRDFPENL---------- 387

  Fly   410 GLLANNEKNAADIGVQTDPELVEIGIDQRTNGNTINRTK----TIILQNSNKVQAFINEWFMEHP 470
                   :.:.|:            |..|.:|::.....    .:..|:|:.|       ..:..
Zfish   388 -------QRSGDV------------IPPRGSGSSYRSPSHPLPPVRKQSSSPV-------LPDSL 426

  Fly   471 SGKVHQILIV-IVLGMIALF-----W----------------------YTCSTMRELQ------- 500
            |.:...:|:| ::||....|     |                      |:.||  ||.
Zfish   427 SSEPMTVLVVTLLLGAWITFLLTHKWPVKKSQRSEEPVDSTPLSGLTNYSAST--ELNTPPSTSS 489

  Fly   501 -KQSENGSKTFAIAQN-----GSNGSTGSNGSNANAEDLVDLGNGQVRVGKISFSTNEVLGKGCE 559
             ..|....||.::|.|     .|..|.....::.:..:..|:    |.|||||||..||||.|.|
Zfish   490 YSNSSRSEKTSSVASNQTQPFSSKDSAPVTAASQSQNEQADV----VEVGKISFSPAEVLGHGTE 550

  Fly   560 GTFVFKGTFEERFVAVKRLLPECFTFADREVALLRESDAHENVVRYFCTEQDRQFRYIAVELCAA 624
            |||||:|.|:.|.|||||:||||..||:|||.||||||.|.||:||||||:||||.|||:|||||
Zfish   551 GTFVFRGHFDGRRVAVKRILPECVEFAEREVQLLRESDEHPNVIRYFCTERDRQFTYIAIELCAA 615

  Fly   625 TLQDYTEGDRSLELQNHIDVWQVLSQAASGLSHLHSLDIVHRDIKPQNVLISLPDAKGKVRVMIS 689
            |||.|.| |.|.. .:.::...:|.|...||||||||:|||||:||:|:|:|||.|.|:||.:||
Zfish   616 TLQQYVE-DPSCP-HSELNPVSLLEQTMCGLSHLHSLNIVHRDLKPRNILLSLPGALGRVRALIS 678

  Fly   690 DFGLCKKLNFGKTSFSRRSGVTGTDGWIAPEMMRSQ---RTTTAVDIFSLGCVYYYVLSGGHHAF 751
            ||||||||..|:.|||.|||:.||:||||||::.:.   ..|:||||||.|||:|||.|.|.|.|
Zfish   679 DFGLCKKLPDGRHSFSLRSGIPGTEGWIAPELLINAPKGNPTSAVDIFSAGCVFYYVTSKGQHPF 743

  Fly   752 GDNLKRQANILSHEYNLAKLRPEDDSEDSRIILAEQLISDMIHKDPQSRPPARCIGNHPLFWDEP 816
            ||.|:|||||||..|||.... ||..||   ::...||..||..:|:|||.|..|..||.||...
Zfish   744 GDTLRRQANILSGVYNLDHFM-EDIHED---VIGRDLIERMISAEPESRPSAASILKHPFFWSPE 804

  Fly   817 KMLSFLQDVSDRVEKLQFHAEPLKS-----LEKNGRIVVLDDWNVHLDPMITDDLRKYRGYMGAS 876
            |.|.|.||||||:||     ||.:|     ||.:||.||..:|.:|:...:..||||:|.|.|.|
Zfish   805 KQLQFFQDVSDRIEK-----EPTESPIVARLENSGRSVVRTNWRMHISAPLQADLRKFRTYKGNS 864

  Fly   877 VRDLLRALRNKKHHYHELTPAAQKMLGCIPHEFTNYWVDRFPQLISHAYHAFSICSNEPIFKPYY 941
            |||||||:||||||||||.|..|..||.:|..|..|:..|||:|:.|.:.|.|||:.|..|.|||
Zfish   865 VRDLLRAMRNKKHHYHELPPEVQSALGEVPDGFVAYFTSRFPRLLLHTHTALSICAPERPFHPYY 929

  Fly   942  941
            Zfish   930  929

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ire1	NP_001097839.1	Luminal_IRE1	53..321	CDD:188875	107/286 (37%)
		STKc_IRE1	545..813	CDD:270884	164/270 (61%)
		RNase_Ire1	817..941	CDD:199217	66/128 (52%)
ern2	XP_001919350.4	None