Protein Alignment ClC-a and GEF1

DIOPT Version :10

• Sequence 1: NP_788639.1
• Gene: ClC-a / 41428
• FlyBaseID: FBgn0051116
• Length: 1193
• Species: Drosophila melanogaster
• Sequence 2: NP_012574.1
• Gene: GEF1 / 853497
• SGDID: S000003801
• Length: 779
• Species: Saccharomyces cerevisiae
• Alignment Length: 772
• Identity: 198/772 - (25%)
• Similarity: 318/772 - (41%)
• Gaps: 168/772 - (21%)

  Fly    98 DDPIG----YIDTLMYGRY--TKDLGEFA-----KDEARKLKILEKRRKQEDKQRN-KELLGKHS 150
            :.|||    .|||..:...  |::..:|.     .:|.|.|.:...|.....|.|: :|::
Yeast     9 NQPIGDGEDVIDTNRFTNIPETQNFDQFVTIDKIAEENRPLSVDSDREFLNSKYRHYREVI---- 69

  Fly   151 TRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALL-----------------SFIMDKGISICTN 198
                    |....|...|....:   ::|.|...|                 :::::|  |.|.|
Yeast    70 --------WDRAKTFITLSSTAI---VIGCIAGFLQVFTETLVNWKTGHCQRNWLLNK--SFCCN 121

  Fly   199 ARIWLYRDLTS--------QPFV-----QYIAW----------VSLPVCLILFSAGFVHLIAPQS 240
            .   :..::||        |.|.     .:|||          :.|.|...|.|...|..:||.:
Yeast   122 G---VVNEVTSTSNLLLKRQEFECEAQGLWIAWKGHVSPFIIFMLLSVLFALISTLLVKYVAPMA 183

  Fly   241 IGSGIPEMKTILRGVQL-KEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLV 304
            .||||.|:|..:.|.:. ||:|.|.|||.|.:.|...:.||:.:|||||.||.|:....||:|.:
Yeast   184 TGSGISEIKVWVSGFEYNKEFLGFLTLVIKSVALPLAISSGLSVGKEGPSVHYATCCGYLLTKWL 248

  Fly   305 TSFQGIYENESRNSEMLAAACAVGVGACFAAPVGGVLFSIE--VTTTYFAVRNYWRGFFAAVCGA 367
            ......|   |...|.:.||...||...|.||:|||||.:|  .:...|.....|:.::.|:...
Yeast   249 LRDTLTY---SSQYEYITAASGAGVAVAFGAPIGGVLFGLEEIASANRFNSSTLWKSYYVALVAI 310

  Fly   368 TVFRLLAVWFQNADTVRALFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYV--W----VHRRY 426
            |..:.:.. |:|...:    |.|.|.:..:..||:.:|..:|:..||.| .|:  |    :|.|.
Yeast   311 TTLKYIDP-FRNGRVI----LFNVTYDRDWKVQEIPIFIALGIFGGLYG-KYISKWNINFIHFRK 369

  Fly   427 VLFMRSNKRMNKFLQKNRFLYPGFLALLVSSISFPLGTGQFLAGELST------HEQV----TQL 481
            :..  |:..:.:.|         |||.|.:.||:   ..:||..:::.      ||.|    |..
Yeast   370 MYL--SSWPVQEVL---------FLATLTALISY---FNEFLKLDMTESMGILFHECVKNDNTST 420

  Fly   482 FSNFTWSRDDLTVEQAAVVTHWMTSYTSVFGNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKIGA 546
            ||:.....|:        .|| ...:..:|.:|...|:...::.:::....||:|:|:|...:||
Yeast   421 FSHRLCQLDE--------NTH-AFEFLKIFTSLCFATVIRALLVVVSYGARVPAGIFVPSMAVGA 476

  Fly   547 GFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAA-FSGSVTHTVSVAVIIFEMTGQITHV 610
            .|||.|...:.       |:....|.|.||.||.:|||| .||....|::|.||:||:||...::
Yeast   477 TFGRAVSLLVE-------RFISGPSVITPGAYAFLGAAATLSGITNLTLTVVVIMFELTGAFMYI 534

  Fly   611 VPVMIAVLVANAVAAL--LQPSIYDSIILIKKLPYLPDLLPSSSGMYSIFVEDFMVRDVKYIWHG 673
            :|:||.|.:...:.:.  :...|.|.:|::...|||.|......       |:.:   .||....
Yeast   535 IPLMIVVAITRIILSTSGISGGIADQMIMVNGFPYLEDEQDEEE-------EETL---EKYTAEQ 589

  Fly   674 ISYQKLKEVLKLNKT-----LRSLPLVDSPDNMILLGSVQRYELIKMIEKH------IGREKRME 727
            :...||   :.:|:|     |.|| |.||....    ||..:.:.|..:|.      ||...:..
Yeast   590 LMSSKL---ITINETIYLSELESL-LYDSASEY----SVHGFPITKDEDKFEKEKRCIGYVLKRH 646

  Fly   728 VAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKK 784
            :|.|...::      ....|.:..|.....|..|:....:.:..:.|.|:..:..||
Yeast   647 LASKIMMQS------VNSTKAQTTLVYFNKSNEELGHRENCIGFKDIMNESPISVKK 697

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ClC-a	NP_788639.1	ClC_1_like	171..646	CDD:239655	150/536 (28%)
		CBS_pair_SF	659..>718	CDD:449531	16/63 (25%)
		CBS repeat	664..715	CDD:341358	14/55 (25%)
		CBS_pair_SF	<1032..1096	CDD:449531
		CBS repeat	1053..1095	CDD:341358
GEF1	NP_012574.1	ClC_3_like	83..569	CDD:239656	147/532 (28%)
		CBS_pair_voltage-gated_CLC_euk_bac	586..736	CDD:341367	26/126 (21%)
		CBS repeat	592..687	CDD:341367	22/108 (20%)
		CBS repeat	693..735	CDD:341367	2/5 (40%)