Protein Alignment pug and mthfd1a

DIOPT Version :10

• Sequence 1: NP_731489.2
• Gene: pug / 41279
• FlyBaseID: FBgn0020385
• Length: 968
• Species: Drosophila melanogaster
• Sequence 2: XP_695978.4
• Gene: mthfd1a / 567587
• ZFINID: ZDB-GENE-041001-127
• Length: 919
• Species: Danio rerio
• Alignment Length: 925
• Identity: 450/925 - (48%)
• Similarity: 611/925 - (66%)
• Gaps: 32/925 - (3%)

  Fly    38 AKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAA 102
            |.::||...::.:::.|:.||..|..|...|.|||.::|||.|||||:||.||:|||.||||:|.
Zfish    25 ATVVSGNKTSQQVKQRLKEEVEQMKSQFPGFRPGLVVLQVGDREDSNLYISMKLKAAAEIGINAN 89

  Fly   103 HVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEG 167
            |::||.:.||.|:|..|..:||:|..||:|||:|||...||::.:||:||:||||||||.::|.|
Zfish    90 HIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAG 154

  Fly   168 RLAIGDLGG-FLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSK 231
            :|:.||||. |:||||.||:|||.::||.:.|..|||:|||||||.|..:||.|::||||.||||
Zfish   155 KLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSK 219

  Fly   232 TRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQ 296
            |.:|.|....|||||||.|.||||||.|:|.||||||||||..||.|||||.::||||.|..|.|
Zfish   220 TPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQ 284

  Fly   297 VAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKP 361
            .||.:||||||||||||||||||||.||.|||:.....:|.|.....:|..|.|||..|:.:.:.
Zfish   285 RAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCES 349

  Fly   362 KDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGL 426
            |.:..||:|:||.:.||..||..:|||.|..|:|::.:..|.||||.|:|.:||||||.||.:||
Zfish   350 KPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGL 414

  Fly   427 VQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAA 491
            .||||||...|..|.:::.|....||.:|.|.||||:|:||:||.:|..:|....||||..|:..
Zfish   415 AQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMD 479

  Fly   492 QLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDI 556
            .:.....:|....:|||:|.|||..:|||..|..||.||:||.|.|:||.|||..|...|.||||
Zfish   480 TVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDI 544

  Fly   557 DPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLAD 621
            |.|                      |.|:         |.|:.:|:||||:||.|.|:|:.|||:
Zfish   545 DTD----------------------SATD---------SFSLENEMMAVLSLSSSEEEMQHRLAN 578

  Fly   622 MVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADE 686
            ::||.:|.|.|:|.:||.|:|.||:|||:||:|.|||::|||||.:|..|..|:|.|..||:||:
Zfish   579 IIVATNKSGDPITTEDLNVSGPLAMLLKEALKPVLMQTVEGTPVFLHTSPLVNVAQGGPSILADK 643

  Fly   687 VGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAP 751
            :.||:||..|||.||:|.|:|:|:|||.||||..||.:|:.:|:|||:|.:||.||..|:..|.|
Zfish   644 MALKIVGSEGFVVTESGHGADVGLEKFFNIKCLYSGLQPDVLVMVATIRTLKMQGGAPPIQAGLP 708

  Fly   752 LNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAV 816
            |.|:|.:|||:.:::|..::.:.:|..:|||:||:|::|..|:||.||.|||...|.:||||.||
Zfish   709 LPKEYLQENLKQLERGCCHMRRQVEKAQAFGLPVIVAVNKFSSDTDAELELVCGQARQAGAFDAV 773

  Fly   817 VSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEK 881
            ..:.|::|||||:.||:.|.:|.:....|:.||||:.|..||:..:|..|||...|.|||.|:||
Zfish   774 QCSSWSEGGAGALALAETVQRAAKLPACFKFLYDLQTPASDKLRTVAQKMYGLKDVKLSPKAKEK 838

  Fly   882 VKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGL 946
            :...|..||.|||:|::|...|.:.|..:||:...|.|.:.|:..:|||||:..:.|........
Zfish   839 LALYTKQGFENLPVCIAKTPLSLSNDPMMKGSSTDFMLPITDIKANAGAGFLCCLTGSTNAEMDD 903

  Fly   947 PTRPAIYDIDLNTET 961
            |..|..:..:|.:::
Zfish   904 PMWPCFHGNNLYSDS 918

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_731489.2	FolD	35..326	CDD:439960	174/288 (60%)
		FTHFS	350..968	CDD:460143	269/612 (44%)
mthfd1a	XP_695978.4	FolD	25..314	CDD:439960	174/288 (60%)
		P-loop containing Nucleoside Triphosphate Hydrolases	327..910	CDD:476819	270/613 (44%)