Protein Alignment Ufl1 and UFL1

DIOPT Version :10

• Sequence 1: NP_649690.1
• Gene: Ufl1 / 40843
• FlyBaseID: FBgn0037467
• Length: 782
• Species: Drosophila melanogaster
• Sequence 2: NP_056138.1
• Gene: UFL1 / 23376
• HGNCID: 23039
• Length: 794
• Species: Homo sapiens
• Alignment Length: 813
• Identity: 290/813 - (35%)
• Similarity: 471/813 - (57%)
• Gaps: 53/813 - (6%)

  Fly     1 MGSDWDEIKRLAADFQKAQLTSTLQKLSERNCVEIVTLLLEKQMLEVVFTNDGKEYITPDHLERE 65
            |...|:||:|||||||:||.....|:||||||:|||..|:.::.||||.|.||||||||..:.:|
Human     1 MADAWEEIRRLAADFQRAQFAEATQRLSERNCIEIVNKLIAQKQLEVVHTLDGKEYITPAQISKE 65

  Fly    66 IQDELYVNGGRANLVEVSKTLNVDLSRIELLAERISAENQSVHLVLGQLIDEDYISHIAQEINEK 130
            ::|||:|.|||.|:|::.:.:||||..||.....|....:.|.|||||||||:|:..:|:|:|:|
Human    66 MRDELHVRGGRVNIVDLQQVINVDLIHIENRIGDIIKSEKHVQLVLGQLIDENYLDRLAEEVNDK 130

  Fly   131 LVQRGEVSISELASQFDLPSDFLQHDVVEKHLGKIIKGRQDASNPRVFFTQAYIQRCKAKIRGAL 195
            |.:.|:|:||||...:|||.:||...:.:: ||:||.|..|..|..|.||:|::.|.||:|||..
Human   131 LQESGQVTISELCKTYDLPGNFLTQALTQR-LGRIISGHIDLDNRGVIFTEAFVARHKARIRGLF 194

  Fly   196 AAITRPINVAVILQKIGVQEKIFYSLLDEIAPAGQ-----VTSKQANSQYVPHIYAKTQADWVNS 255
            :|||||..|..::.|.|.||::.||:|:|:..:|:     |..:|..:.:||.||::||:.||:|
Human   195 SAITRPTAVNSLISKYGFQEQLLYSVLEELVNSGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDS 259

  Fly   256 FYKQNSFLEYDAIQKLGISDAKSYIRKQFPNEEFLYLKRVALGARLVELTVVTALNECSATKQYL 320
            |::||.:||:||:.:|||.||.|||:|::...:.|:||...:|..||: .|..::.|..::..::
Human   260 FFRQNGYLEFDALSRLGIPDAVSYIKKRYKTTQLLFLKAACVGQGLVD-QVEASVEEAISSGTWV 323

  Fly   321 DLTTILPSNLSEEDIEEVFSTIMAQKHSNPSNFVYLDGIVFSQPYLAQLVQPCQALAESQAKAAI 385
            |:..:||::||.||...:...:|.......|..|:.|.:|.|:.::....:..:.|...:|:..:
Human   324 DIAPLLPTSLSVEDAAILLQQVMRAFSKQASTVVFSDTVVVSEKFINDCTELFRELMHQKAEKEM 388

  Fly   386 DGGVYQQYIVEKTLAQKGNVSTQELEDEGKVDKRDERRKKASSG----KAGGGAQGRETKTKSTK 446
            ..... ..|.|:.|.|   :||.|.....|.||:||||:||:.|    :.|||...||.|.|..|
Human   389 KNNPV-HLITEEDLKQ---ISTLESVSTSKKDKKDERRRKATEGSGSMRGGGGGNAREYKIKKVK 449

  Fly   447 KHQRGKAAAQFDSDDEDDAQQGSRGGGGASKKAVKP-LELVKTADIVKLITASLEEEGLEHLSKS 510
            |..|       ..||.||..|.|..|    ||  || :..:...:|...:...:::...|.:|:
Human   450 KKGR-------KDDDSDDESQSSHTG----KK--KPEISFMFQDEIEDFLRKHIQDAPEEFISE- 500

  Fly   511 IASLYTNQFNQTALARAQELFEATPQ----TNRRQTHAAIQDRINTLLIDIRLYEKGLKLFPQDT 571
            :|.......|:|.|...:.:|.::..    |.|::|...:|:.::.|..:|||:|||:|.|..||
Human   501 LAEYLIKPLNKTYLEVVRSVFMSSTTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKFFADDT 565

  Fly   572 QTQLVKYLLKSLGNEICNELSLYVASECNLTVKN-TNLNVDQRNKLAQECEAQYRAALLEQNKAL 635
            |..|.|:||||:..:|.|.:..::||:..:.|.: ..:..:.|.|:..:...:.:.||.:.:.:|
Human   566 QAALTKHLLKSVCTDITNLIFNFLASDLMMAVDDPAAITSEIRKKILSKLSEETKVALTKLHNSL 630

  Fly   636 N-KSIDDFELATETVLKTCSMIIKKVDKKKDRLLIADHKKKLQKQLLECNEPALLLHLAALILFT 699
            | |||:||....::..:.|.:::|:.|||::|.::..|::.|.:||....:|||:|||.:::||.
Human   631 NEKSIEDFISCLDSAAEACDIMVKRGDKKRERQILFQHRQALAEQLKVTEDPALILHLTSVLLFQ 695

  Fly   700 TITGSILHASGKFVSAILQHIRGSLNEDQNALLLRYHDLVLQVLQA---------IPDSNE---- 751
            ..|.|:|||.|:.|..|:..:...:.|||:|||::|..||::.|.:         .|.:||
Human   696 FSTHSMLHAPGRCVPQIIAFLNSKIPEDQHALLVKYQGLVVKQLVSQSKKTGQGDYPLNNELDKE 760

  Fly   752 ----SKLANEHLQTMQNQVVELAQNFSRASISK 780
                :....:.||.:.:.:.:|.....::|:::
Human   761 QEDVASTTRKELQELSSSIKDLVLKSRKSSVTE 793

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ufl1	NP_649690.1	E3_UFM1_ligase	7..280	CDD:462874	135/277 (49%)
UFL1	NP_056138.1	Required for E3 UFM1-protein ligase activity. /evidence=ECO:0000269|PubMed:20018847	2..212		104/210 (50%)
		Mediates interaction with DDRGK1. /evidence=ECO:0000269|PubMed:25219498	2..200		100/198 (51%)
		E3_UFM1_ligase	7..284	CDD:462874	135/277 (49%)
		Involved in CDK5RAP3-binding. /evidence=ECO:0000269|PubMed:20164180	121..250		53/129 (41%)
		Mediates interaction with TRIP4. /evidence=ECO:0000269|PubMed:25219498	200..400		63/201 (31%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	407..473		31/78 (40%)
		Mediates interaction with CDK5RAP3. /evidence=ECO:0000250|UniProtKB:B2GV24	490..684		56/194 (29%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	745..770		3/24 (13%)