Protein Alignment PEK and GCN2

DIOPT Version :10

• Sequence 1: NP_649538.1
• Gene: PEK / 40653
• FlyBaseID: FBgn0037327
• Length: 1162
• Species: Drosophila melanogaster
• Sequence 2: NP_010569.3
• Gene: GCN2 / 851877
• SGDID: S000002691
• Length: 1659
• Species: Saccharomyces cerevisiae
• Alignment Length: 1095
• Identity: 250/1095 - (22%)
• Similarity: 399/1095 - (36%)
• Gaps: 369/1095 - (33%)

  Fly   210 EEDQL----EDGEQLRDEAGYIVRHDPLLDDVIIVRRQTQTVRAVESRTGVERWNFSVGQHEL-- 268
            |:|:|    |..|||..|..               .:|.:|::   .|:..:|....:.|.||
Yeast   138 EDDRLQRIKETKEQLEKEER---------------EKQQETIK---KRSDEQRRIDEIVQRELEK 184

  Fly   269 ------DLV--RPSECQLQPRDELELAVLDVDIKVVVPEGIICAFSKS----EPQTMLWKYKFDH 321
                  ||:  |.::..|||..|.            |..|....|||:    .|...::|:|
Yeast   185 RQDDDDDLLFNRTTQLDLQPPSEW------------VASGEAIVFSKTIKAKLPNNSMFKFK--- 234

  Fly   322 PIVSAWNTNADDELQPI----DLFS-SAQWLWDQDENDTELPNAPQSPPSIYL---GMYDKQLYI 378
            .:|:.         :||    |:|| |.|:|     ....:|  |:||.:.:|   .|.:...|:
Yeast   235 AVVNP---------KPIKLTSDIFSFSKQFL-----VKPYIP--PESPLADFLMSSEMMENFYYL 283

  Fly   379 QESIRLRQEIMDQTKVYQQLTGDTSLMPRIPWKPISASSKSLVIFRKDQEDPEMIAEGAVAQGGE 443
            ...|.|      ....:....|.         |.|:...|.|....|.:.|          ....
Yeast   284 LSEIEL------DNSYFNTSNGK---------KEIANLEKELETVLKAKHD----------NVNR 323

  Fly   444 LVPYDDE----NFAVAAQSVLNASEFVNGNGFYFYTTGDLNGPQECSTQNNPTDLPAITAPTSPT 504
            |..|..|    |.|.....:...:|:.|     :|..|||                 |.:.....
Yeast   324 LFGYTVERMGRNNATFVWKIRLLTEYCN-----YYPLGDL-----------------IQSVGFVN 366

  Fly   505 NATS--------EGTEATG-----NHSVNDDLGFSLDDID--APVKVVILSLWFW---------- 544
            .||:        ||.||..     :..:|.:....:.|.|  :.:..::.|.:.:
Yeast   367 LATARIWMIRLLEGLEAIHKLGIVHKCINLETVILVKDADFGSTIPKLVHSTYGYTVLNMLSRYP 431

  Fly   545 -------------WKEIVVIAFTSA-------------VILNIFMGQRNQRVEREYLVIERHVPV 583
                         |....::.|.:|             :.:.|..|..        :|:....|
Yeast   432 NKNGSSVELSPSTWIAPELLKFNNAKPQRLTDIWQLGVLFIQIISGSD--------IVMNFETP- 487

  Fly   584 QTAIEAT--EASTQALLGPVV---PMQRPG------------------NRFSFPPGQANQ----- 620
            |..:::|  :.:...||..::   |.:|.|                  |||:......|.
Yeast   488 QEFLDSTSMDETLYDLLSKMLNNDPKKRLGTLELLPMKFLRTNIDSTINRFNLVSESVNSNSLEL 552

  Fly   621 -------------RTISESTT-----HSGEHYT-------SRFQSDFELMQCLGRGGFGVVFEAK 660
                         ||:|:|:.     :.|..::       ||:.||||.:..||:|.||.|.:|:
Yeast   553 TPGDTITVRGNGGRTLSQSSIRRRSFNVGSRFSSINPATRSRYASDFEEIAVLGQGAFGQVVKAR 617

  Fly   661 NKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTETPPTGWQEEEDRKLLA 725
            |.||...||||:|.  :.|.....:|.|...|||..|..:|||:.:|.         |||
Yeast   618 NALDSRYYAIKKIR--HTEEKLSTILSEVMLLASLNHQYVVRYYAAWL---------EED----- 666

  Fly   726 HELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHLPGESSLKNIREEYD 790
                              |:.|.:.|...::     ||.:.|::...:..|..:||:...|..:|
Yeast   667 ------------------SMDENVFESTDEE-----SDLSESSSDFEENDLLDQSSIFKNRTNHD 708

  Fly   791 YDEEEDSLIEFRSESQSAALRAEEEDDTDDDYEEDEEQQGDHEKRHRSSVSIDIHSASFDLKNIN 855
            .|   :|..:|.|.|....:..|...      .:||.:..||:....||
Yeast   709 LD---NSNWDFISGSGYPDIVFENSS------RDDENEDLDHDTSSTSS---------------- 748

  Fly   856 YSQHQLVSNSFQIESVRPKSSGSDDANDDNKARRKPLTLALAQNHNNNQNGSQPTPSSATILNGT 920
                               |...||.:.::|:         .||....:|..:|          .
Yeast   749 -------------------SESQDDTDKESKS---------IQNVPRRRNFVKP----------M 775

  Fly   921 VAKPSKVYLYIQMQLCRKESLRDWLRDNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSN 985
            .|...|..|:|||:.|...:|.|.:.......:......:|.||::|:.|:|.:|:|||||||.|
Yeast   776 TAVKKKSTLFIQMEYCENRTLYDLIHSENLNQQRDEYWRLFRQILEALSYIHSQGIIHRDLKPMN 840

  Fly   986 IFFSQDGQIKIGDFGLVTDM---ADIPNLVAKCGDQSGLPSCARH-TQQVGTHLYMSPEQLLGQ- 1045
            ||..:...:|||||||..::   .||..|     |...||..:.: |..:||.:|::.|.|.|.
Yeast   841 IFIDESRNVKIGDFGLAKNVHRSLDILKL-----DSQNLPGSSDNLTSAIGTAMYVATEVLDGTG 900

  Fly  1046 HYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDG--QYPKDFAVNYPQ-QYDLLQQMLSAQ 1107
            ||:.|:|:||||:||||:...|||.|||:..|:.||..  ::|.||..|..: :..:::.::...
Yeast   901 HYNEKIDMYSLGIIFFEMIYPFSTGMERVNILKKLRSVSIEFPPDFDDNKMKVEKKIIRLLIDHD 965

  Fly  1108 PEQRPQTKQLKSQLRNILQLPHLLSEGQSEQAELAERARRLSRSRTFSSSSEPHQ 1162
            |.:||..:.|.:.  ..|.:.|        |.|:.:.|     .::.|:.|.|.|
Yeast   966 PNKRPGARTLLNS--GWLPVKH--------QDEVIKEA-----LKSLSNPSSPWQ 1005

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PEK	NP_649538.1	Luminal_EIF2AK3	73..401	CDD:188874	48/216 (22%)
		Protein Kinases, catalytic domain	635..1113	CDD:473864	139/492 (28%)
GCN2	NP_010569.3	RWD_GCN2	12..129	CDD:467659
		PK_eIF2AK_GCN2_rpt1	273..526	CDD:270914	47/308 (15%)
		STKc_EIF2AK4_GCN2_rpt2	593..979	CDD:270948	141/494 (29%)
		HisRS-like_core	1038..1362	CDD:238396
		HGTP_anticodon2	1387..1657	CDD:432758