Protein Alignment polo and PLK1

DIOPT Version :10

• Sequence 1: NP_524179.2
• Gene: polo / 40232
• FlyBaseID: FBgn0003124
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: NP_005021.2
• Gene: PLK1 / 5347
• HGNCID: 9077
• Length: 603
• Species: Homo sapiens
• Alignment Length: 581
• Identity: 305/581 - (52%)
• Similarity: 415/581 - (71%)
• Gaps: 32/581 - (5%)

  Fly     2 AAKPEDKSTDIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQ 66
            ||.|..|  :||:.|||...|:.|.|.||.||||||||:||.|.:|.:|||||||.|.|::|.:|
Human    32 AAAPPAK--EIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQ 94

  Fly    67 KEKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQ 131
            :||.:.||:|||||.|.::|.||.:|||:..:::|||||::||::|||||||::||.|.|||:.|
Human    95 REKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQ 159

  Fly   132 IIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILT 196
            |:.|.:|||.||:|||||||||||||:.|.||||||||||::||:|||||||||||||||||:|:
Human   160 IVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLS 224

  Fly   197 KKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPN 261
            |||||||||:|||||:|||||||:|||||..||:||.:|||.||.:|.::...||.::..|||.:
Human   225 KKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTD 289

  Fly   262 PESRPAIGQLLNFEFLKGSKVPMFLPSSCLTMAPRIGSNDTIEDSMHRKPLMEMN-GIR---PDD 322
            |.:||.|.:|||.||.....:|..||.:|||:.||.....:..|..:||||..:| |:.   |:.
Human   290 PTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPER 354

  Fly   323 TRLESTFLKANLHDAITASAQV--CRHSEDYRSDIESLYQQLTNLINGKPR----ILQGNLGDEN 381
            .|.:.       ...:..:.:|  |..|:        :.|||.::...||.    :.|    :|.
Human   355 PREKE-------EPVVRETGEVVDCHLSD--------MLQQLHSVNASKPSERGLVRQ----EEA 400

  Fly   382 TDPAAQPLFWISKWVDYSDKYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTT 446
            .|||..|:||:|||||||||||.||||||..:||:|||:|:|||..:..::.:|::||.|:|:|.
Human   401 EDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTV 465

  Fly   447 TDYCKSLDKKMKLLSYFKRYMIEHLVKAGANNVNIESDQISRMPHLHSWFRTTCAVVMHLTNGSV 511
            :.:..||.||:.||.||:.||.|||:|||||....|.|:::|:|:|.:||||..|:::||:||||
Human   466 SSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSV 530

  Fly   512 QLN-FSDHMKLILCPRMSAITYMDQEKNFRTYRFSTIVENGVSKDLYQKIRYAQEKLRKML 571
            |:| |.||.||||||.|:|:||:|::::|||||.|.:.|.|..|:|..::|||:..:.|:|
Human   531 QINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLL 591

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
polo	NP_524179.2	STKc_PLK	23..277	CDD:271001	164/253 (65%)
		POLO_box_1	388..475	CDD:240561	49/86 (57%)
		POLO_box_2	490..570	CDD:240560	42/80 (53%)
PLK1	NP_005021.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..35		2/2 (100%)
		STKc_PLK1	45..309	CDD:271089	168/263 (64%)
		Activation loop	194..221		23/26 (88%)
		D-box that targets the protein for proteasomal degradation in anaphase	337..340		2/2 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	338..364		7/32 (22%)
		POLO_box_1	407..494	CDD:240561	49/86 (57%)
		Linker	493..507		6/13 (46%)
		POLO_box_2	509..590	CDD:240560	42/80 (53%)
		Important for interaction with phosphorylated proteins. /evidence=ECO:0000250	538..540		1/1 (100%)