Protein Alignment gig and Tsc2

DIOPT Version :10

• Sequence 1: NP_524177.1
• Gene: gig / 40201
• FlyBaseID: FBgn0005198
• Length: 1847
• Species: Drosophila melanogaster
• Sequence 2: NP_001273649.1
• Gene: Tsc2 / 22084
• MGIID: 102548
• Length: 1814
• Species: Mus musculus
• Alignment Length: 1995
• Identity: 597/1995 - (29%)
• Similarity: 944/1995 - (47%)
• Gaps: 407/1995 - (20%)

  Fly     3 SKD---KSKFKLFL-----KSLPAGYVGERT---LRPEFERELRPEQPVAQRCRMLKELGDTQLH 56
            |||   |.|||:.|     :..|....|::|   :..|..|||..|..:..|.||:.::.|. ..
Mouse     6 SKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDV-AK 69

  Fly    57 NFNLDESAITILFNLTNDLIVPNKPAETRQIALSFYKRLIHTQYKNLTIMREKFFLVIQNHEARE 121
            ...|:|.|:..|:...:||:.|.:|.|.|...|:..|.::..|...|.::|..||.||:::.:.|
Mouse    70 TKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNE 134

  Fly   122 DLRHLLELLDTLTDNGKDITNFEEKIGKFMLLWIPAITEANLLTPYLDILVNLIKFNAAHLDKDI 186
            ||...||:...|||||:.||..||::.:|:|.|:    :..|.:.:|.:||||:|||:.:|| :.
Mouse   135 DLHERLEVFKALTDNGRHITYLEEELAEFVLQWM----DVGLSSEFLLVLVNLVKFNSCYLD-EY 194

  Fly   187 LVGIVQNACDLSCSVTVNEIGLQ-CLTILEMVIGYTIFPSEPLPQCITTLCRTVNHAPYCPSSFK 250
            :..:|...| |.|..||:.:.:: .|.:|:.|:.|...|:|.||..|.|||||:|....|...:|
Mouse   195 IASMVHMIC-LLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWK 258

  Fly   251 IMKNLLGTQLGYHSMKMMCSILNDRALYDDAHLLRGAVFHLNMNIFGSNIIFQV--SPMTYATNV 313
            :|:|||||.||:.::..||.|:.||:..:||.|||||||.:.|.::|::.::.:  ||    |:|
Mouse   259 LMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSP----TSV 319

  Fly   314 LTAFLRALDSRQVIVTFEVILSVRMVITK--RQLSEIIWDLICDIMSSIVSNIEYYEEVNINKDR 376
            |.:|..|:.....:|::|::||:..:|.|  ::|..:.||::.||:..::..::     |::...
Mouse   320 LPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQ-----NLDSPE 379

  Fly   377 LHHLQINFHENIDCIEKLLQQDRSQILGNVERIYDLIERVADRRPEASVLALIEYRSRRVTATRP 441
            |..:   .|:.:..:|:|.  |:::..|:.||.|:|:|..||:|||:|:|.||.||::.:...:.
Mouse   380 LKTI---VHDLLTTVEELC--DQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKD 439

  Fly   442 DWLQVLAQFVRRYYRMS-NVNVRIKTIEALVQIMDQNRACYEEEILSRVVLVHLSHIHLESSVQV 505
            .|:|.|...:.|::|.. ...||||.::.|..::..||..||||:::.||:..||||..:...||
Mouse   440 GWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQV 504

  Fly   506 RVAVARALSNFATHCDTKRCMDLLDILEALINRPFEHTRHGSSSGEGTLAEVTFGLVNNESEISD 570
            |....:.|.:.|..|.|.....||||:|.::.|        |.|....|.|  ..|..:.:.:.|
Mouse   505 RKLATQLLVDLAEGCHTHHFNSLLDIIEKVMAR--------SLSPPPELEE--RDLAMHSASLED 559

  Fly   571 IIAAVDGLVKVFAIKLHRLPGIHALKIFNILMDHLELHYERPKIFEHISVVRYKIFAWLLKARAN 635
            :..||.||:.:...||:.||..||.:::..|:.|::|||:........|.:|.:.|.:||..||:
Mouse   560 VKTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRAD 624

  Fly   636 GSYHIGYPEGSTEVVKFSPYLGID-------------SPLLPQAHPTA------------ISIRR 675
            ..:.:|.| ....||:||||...|             .||.|...|.:            :....
Mouse   625 SLHRLGLP-NKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSL 688

  Fly   676 VCKLIVRCLEHDTDYQVFQLVIRELPKVLQNKALVQGN--DIEELANTLLKINLVSNNK------ 732
            :.:::::||:.::|::|.:||:..||:.|:.|.|:..:  .:::|::.|  .:::|..|
Mouse   689 LFRVLLQCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSAL--CSMLSAPKTLERLR 751

  Fly   733 -----FKRPTDEFHALVLPAIASLVIYHESLQPQQHYGIITALNSRVLTGIASVCINTMTILILE 792
                 |.| || .|..|:|.:.:|:.||..|...:...::..|...::...||.|:..:.|..:|
Mouse   752 GTPEGFSR-TD-LHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVE 814

  Fly   793 MPEALMRKLPDVLLQMSKMSDTNALATPVLEFLSLLIHLSKHLFANFTSMHNMYVFAITLPYTKP 857
            ||:.:::.||.::::::.:|.|.::|.|:|||||.|..| .||:.||.:.....||||:||||.|
Mouse   815 MPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARL-PHLYRNFAAEQYASVFAISLPYTNP 878

  Fly   858 HRYDHYTVSLAHHVIAGWFLKCKLELRRNCVNYIKSSIQSNAKMLSSDIVNLNSLNEDSSNRKRS 922
            .:::.|.|.|||||||.||::|:|..|::.|.||...::||.      :::.:...|..|.|.||
Mouse   879 SKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNV------LLSFDDTPEKDSFRARS 937

  Fly   923 TSLTER------------GSRNN-----------ANAWN-------------------------- 938
            |||.||            |..|:           |.|:.
Mouse   938 TSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGS 1002

  Fly   939 -DLEMRPQMNNGLRNFHAELAETCFDFLARHTYSPCPSMPKRLPAAEFLLKDGVSQTWLVGNNLV 1002
             |.....|.::.|:|.|.||.|||.|.:||:.:|...::|||.|..||||..|.::||||||.||
Mouse  1003 ADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLV 1067

  Fly  1003 TITTSGCPSAPARSGLCERCAQLGKAPSISLNSKSLSDAAPPLSPERERRYTKVSLQHSSGNE-S 1066
            |:|||......:..||.....|.|        |.|.||   |.:..|:.:.....|:..:|.: |
Mouse  1068 TVTTSVGTGTRSLLGLDSGDLQGG--------SDSSSD---PSTHVRQTKEAPAKLESQAGQQVS 1121

  Fly  1067 AGSTELTSSSSSNSAAAGGH-----------PHRQISNSSTASLDALSRRGSNPEALGSALGEGA 1120
            .|:.:...|.|      |||           |:....::|.....|::.:...|.|     |...
Mouse  1122 RGARDRVRSMS------GGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPA-----GAQL 1175

  Fly  1121 HTGSNTSLLGNSLSQSSVSMSPSSGSVVQQPVCVRACTGWAEVLVRRPTGNVSWITRIQNPIT-- 1183
            .|...|:|                  ....|:..:   ||||:||||||||.||:..::||::
Mouse  1176 PTAEKTNL------------------AAYVPLLTQ---GWAEILVRRPTGNTSWLMSLENPLSPF 1219

  Fly  1184 ----NDCFGQELPFNNVV----------------SLFLPTAHGGVFGPDNTIQAPPHTLADPEPE 1228
                |:...|||  :|.:                ||.:|.|.          .|.|.||  |...
Mouse  1220 SSDINNMPLQEL--SNALMAAERFKEHRDTALYKSLSVPAAG----------TAKPPTL--PRSN 1270

  Fly  1229 PEAEVNAATAPQASALRSRMVAKARALQRQEEIHSVGGNGNGNGNGNGSGNAPSTGGAAAIPIPR 1293
            ..|..::...|.......|.|:.|.:....||                     .:.|...:|
Mouse  1271 TVASFSSLYQPSCQGQLHRSVSWADSAMVLEE---------------------GSPGETQVP--- 1311

  Fly  1294 VPASGKRGKEAALCGSVSDGEADDDSLAFEDAQSRARNPVRRVNSSPEMSSSWRQSFLTNKPTPL 1358
                            |...|.:|    ||.|....|:..|....|...|:|
Mouse  1312 ----------------VEPPELED----FEAALGTDRHCQRPDTYSRSSSAS------------- 1343

  Fly  1359 SQEPVKTTADEPQSLLTLKKKTVQYSTKDMRVSCEAIPEEIAGSTPPSQAAA-LALQP----ESG 1418
            |||. |:..:|                    ::...||.|.|.|:..::.|. |:.||    ...
Mouse  1344 SQEE-KSHLEE--------------------LAAGGIPIERAISSEGARPAVDLSFQPSQPLSKS 1387

  Fly  1419 TLPPKQHSADDVSSHVAGGSNLQAGGSTLKLGKPPLSP-----GQPPLLGTGRVTSFGGT----- 1473
            :..|:..:..|:...:         |..:.:|:  |||     .|..:| .|...::..|
Mouse  1388 SSSPELQTLQDILGDL---------GDKIDIGR--LSPEAKVRSQSGIL-DGEAATWSATGEESR 1440

  Fly  1474 -SVPQPGALAKSSSSGSNGTNVGVITSDYDNGNNGNGDMMRGRSKTISVV------REVNNGNTR 1531
             :||..|.|..||....:|                    :|.|..|||..      :.|...|.:
Mouse  1441 ITVPPEGPLPSSSPRSPSG--------------------LRPRGYTISDSAPSRRGKRVERDNFK 1485

  Fly  1532 PPPASSFRNFGAAKPPINTKLCMNPSFIFMQLYTTGQLG-VTDEPLKVGPEN-SSAVSLIDLVPP 1594
            ...|:|    .|.|.|     .:||||:|:|||.:...| .:::|:.:..|: ..:|.|:|.:|.
Mouse  1486 SRAAAS----SAEKVP-----GINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPS 1541

  Fly  1595 FETHKIGVLYVGQGQCNNEVEILRNSHGSARYVEFLRNIGTLVSLKEAEQNNLFI-MLDRNGADG 1658
            ::||||.|||||:||.::|:.||.|.|||.||.|||..:|.|:.||:.:.:.::: .||..|.||
Mouse  1542 YDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDG 1606

  Fly  1659 KFAYIWKDDILQVTFHVATLMPTNLQDDPNCNEKKSHIGNDFVKIIYNESGEEYNLNTISGQFNY 1723
            :|.|.|.|||:|..||:||||||...|...| :||.|:|||||.||||:|||::.|.||.||||:
Mouse  1607 QFTYCWHDDIMQAVFHIATLMPTKDVDKHRC-DKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNF 1670

  Fly  1724 ACVIVEPLDLNSNRVYVKARSEISKFVCHAEY------------RIVSDRSAPLLARQMALHANL 1776
            ..||:.|||...|.:.::.|.:..  .|..|:            .:|.:.:..:|    ..|..:
Mouse  1671 VHVIITPLDYKCNLLTLQCRKDGP--ACKCEWWRQPGEIVVWALPVVMELTVTIL----LCHLQM 1729

  Fly  1777 ASLVYQSVQKKHP---YASNWLERLRKLKRLRSKLIEGLNSQQKS 1818
            ||.|:.|  :.:|   |.|.|:.|||.:||||.::.|.::....|
Mouse  1730 ASQVHHS--RSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPS 1772

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
gig	NP_524177.1	DUF3384	40..421	CDD:463376	130/385 (34%)
		Tuberin	566..882	CDD:460965	108/353 (31%)
		PRK12727	<1219..>1418	CDD:237182	38/203 (19%)
		Rap_GAP	1617..1802	CDD:460463	81/200 (41%)
Tsc2	NP_001273649.1	DUF3384	54..419	CDD:463376	130/385 (34%)
		Tuberin	555..903	CDD:460965	108/353 (31%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1078..1174		25/117 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1326..1357		10/64 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1429..1491		16/81 (20%)
		Rap_GAP	1564..1756	CDD:460463	81/200 (41%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1772..1798		1/1 (100%)