Protein Alignment ash1 and Nsd2

DIOPT Version :10

• Sequence 1: NP_524160.2
• Gene: ash1 / 40133
• FlyBaseID: FBgn0005386
• Length: 2226
• Species: Drosophila melanogaster
• Sequence 2: NP_001178481.2
• Gene: Nsd2 / 680537
• RGDID: 1307955
• Length: 1365
• Species: Rattus norvegicus
• Alignment Length: 1504
• Identity: 321/1504 - (21%)
• Similarity: 507/1504 - (33%)
• Gaps: 469/1504 - (31%)

  Fly   234 GQTVNAKLAEE---KVAKAKEETLVQTAVPRKRRGRPKKVVPTVPAPG---------NSGPAINE 286
            |:|.|.::..|   .::||:....:|..|.:|..|.  ..:|.:||..         |..|..::
  Rat    33 GKTQNCEVNHECSVFLSKAQLSNSLQEGVMQKFNGH--DALPFLPAEKLKDLTSCVFNGEPGAHD 95

  Fly   287 SA---DSGVISTTSTTQSTTPSPKMQNENAVPTGSLPIASSSKPKIDMAYLDKRMYATERVLYPP 348
            :.   ::..:....|..:|||         :..||..|    |.||...|:
  Rat    96 TKLCFETQEVKGIGTPPNTTP---------IKNGSPEI----KLKITKTYM-------------- 133

  Fly   349 PRSKRRQNNKKTACSSSNKEELQLDPLWREIDVNKKFRLRSMSVGAASGTGASTTICSKVLAAKS 413
                   |.|               ||:.               .:..|.||:....|:    ::
  Rat   134 -------NGK---------------PLFE---------------SSICGDGAADMSQSE----EN 157

  Fly   414 GYVSDYGSVRHQRSSHNHNSGYKS---DASCKSRYSTKSCMSRRSRAKSCGYRSDCKESGKSGLR 475
            |..||..:.|:::.|..::|..:.   :|:..|:.|:..  .::...|    :..|..||
  Rat   158 GQKSDNKTRRNRKRSIKYDSLLEQGLVEAALVSKISSPE--DKKIPVK----KESCPNSG----- 211

  Fly   476 MRRKRRASMLLKSSADDTVEDQDILQLAGLSLGQSSEESNEYISKPSLKSLPTTSASKKYGEINR 540
                |...:|||.:..|.|..    :::|.........::     |.|.:.......||  ...:
  Rat   212 ----RDRDLLLKYNVGDLVWS----KVSGYPWWPCMVSAD-----PLLHNHTKLKGQKK--SARQ 261

  Fly   541 YVTTGQYFGRG--------GSLSATNPDNFISKMMNQRKETPAPSKSSCKIKSRRSSAASMCSSY 597
            |..  |:||..        .||.|...:....|:. |.....||:|:. |||..:..:..:.:.:
  Rat   262 YHV--QFFGDAPERAWIFEKSLVAFEGEEQFEKLC-QESAKQAPTKAE-KIKLLKPISGRLRAQW 322

  Fly   598 VSGV------SRMRRRHRRKSFSH---NKSLNIDSKLLTEIEIIT---------STFNSRCRI-- 642
            ..|:      :.|....|:..|:.   ...|.::.::..|..|.|         |..|....:
  Rat   323 EMGIVQAEEAASMSVEERKAKFTFLYVGDQLRLNPQVAKEAGIATEPLGEMVDSSVANEEAAVDP 387

  Fly   643 ----QDD-------RLTGSSGKEKLLADANKLQAT---LAAPSPAQQLTLNGGGPASTLSKPLKR 693
                ::|       |...||..|....|....::|   :|...|.:.:....|...||.|....|
  Rat   388 GTMREEDIPVKRRRRAKRSSSAENQEGDPGTEKSTPPKMADAEPKRGVGSPAGRKRSTGSASRSR 452

  Fly   694 ------------------------GLKKRKLSEPL-VDFAMLSASASGTPN---------GSGSS 724
                                    ...:.::.|.| ..::||:.......|         .|...
  Rat   453 KGDSAAQFLVFCQKHRDEVVAEHPDASEEEIEELLGSQWSMLNEKQKARYNTKFSLMISAQSEED 517

  Fly   725 NGNT---KRRHKKSQSNDSSSPDDHKLPLKK----RHYLLTPGERPPAEVAFANGKLNAEAWAAA 782
            :|||   ||.|.|...:.....|....|.|:    :|.|     |....:.....:.::.....|
  Rat   518 SGNTSGKKRTHTKRTDDPPEDVDVEDAPRKRLRTDKHSL-----RKRETITDKTARTSSYKAIEA 577

  Fly   783 AAAAKSTASTKSQAQFNARSVKSALTPKKRHLLEQPTSVSGAGSSASNSPLRIVVDNN-SISGGK 846
            |::.||.|:||        ::..|..|.|:......|:.|..|.:.|:||...:.:|. |.:.|.
  Rat   578 ASSLKSQAATK--------NLSDACKPLKKRNRASATASSALGFNKSSSPSASLTENEVSDNPGD 634

  Fly   847 LLDISP---------SSLCSLKQQRRGGAAKQKVSAAKDLVQLQSPAGSY-----PPPGVFEPSV 897
            ....||         .:..|.|:..||.|||::.     :.||....||.     |..|.|..:.
  Rat   635 EPSESPYESADETQTEASVSSKKSERGMAAKKEY-----VCQLCEKTGSLLLCEGPCCGAFHLAC 694

  Fly   898 ELEIQIPLSKLNESVITKAEVESPLLSALDIKEDTKKEVGQRVVETLLHKTGGNLLLKRKRKKIN 962
                 :.||:..|...|..|..|.:.|....|| :|.||                    ||..:|
  Rat   695 -----LGLSQRPEGRFTCTECASGIHSCFVCKE-SKMEV--------------------KRCMVN 733

  Fly   963 RTGFPTVRRKKRKVSVEQQTTAVIDEHEPEFDPDDEPLQSLRETRSSNNVNVQAAPNPPLDCERV 1027
            :.|     :...:..|::....|.:......     ||.|.....:||..|.:.:....:.|.|.
  Rat   734 QCG-----KFYHEACVKKYPLTVFESRGFRC-----PLHSCMSCHASNPSNPRPSKGKMMRCVRC 788

  Fly  1028 PQAGEARETFVARTNQKAPRLSVVALERLQRPQTPARGRPRGRKPKNR----------------- 1075
            |.|....:..:      |...||:|...:     ...|....||.|..
  Rat   789 PVAYHGGDACL------AAGCSVIASNSI-----ICTGHFTARKGKRHHTHVNVSWCFVCSKGGS 842

  Fly  1076 ----EQAEAAPQPPPKSEPEIRPAKKRGRQPKQPVLEEPPPTPPPQQKKNKMEPNIRLPDGIDPN 1136
                |...||..|...|                                      |.:|||
  Rat   843 LLCCEACPAAFHPDCLS--------------------------------------IEMPDG---- 865

  Fly  1137 TNFSCKIRLKRRKNLEAGTQPKKEK------------PVQPVTVEEIPPEIPVSQEEIDAEAEAK 1189
             ::.|       .:..||.:...:.            |.:....:.:||.|...:.||
  Rat   866 -SWFC-------NDCRAGKKLHFQDIIWVKLGNYRWWPAEVCHPKNVPPNIQKMKHEI------- 915

  Fly  1190 RLDSIPTEHDPLPASESHNP----GPQDYASCSES------SEDKAST-TSLRKLSKVKKTYLVA 1243
                            ...|    |.:||....::      ..|:.|. ..:|.:.:|
  Rat   916 ----------------GEFPVFFFGSKDYYWTHQARVFPYMEGDRGSRYQGVRGIGRV------- 957

  Fly  1244 GLFSNHYKQSLMPPPAKVNK-KPGLEEQVGPASLLPPPPYCEKYLRRTEMDFELPYDIWWAYTNS 1307
                  :|.:|....|:.|: |...|.:....|...||||  |:::..:     ||.
  Rat   958 ------FKNALQEAEARFNEIKLQREARETQESERKPPPY--KHIKVNK-----PYG-------- 1001

  Fly  1308 KLPTRNVVPSWNYRKIRTNVYAESVRPNLAGFDHPTCNCKNQGEKSCLDN--CLNRMVYTECSPS 1370
                            :..:|...:.      :.|.||||...|..|..:  |||||:..||.|.
  Rat  1002 ----------------KVQIYTADIS------EIPKCNCKPTDENPCGSDSECLNRMLMFECHPQ 1044

  Fly  1371 NCPAGEKCRNQKIQRHAVAPGVERFMTADKGWGVRTKLPIAKGTYILEYVGEVVTEKEFKQRMAS 1435
            .|||||.|:||...:... |..:...|..||||:..|..|.||.::.|||||::.|:|...|:..
  Rat  1045 VCPAGEYCQNQCFTKRQY-PETKIIKTDGKGWGLVAKRDIRKGEFVNEYVGELIDEEECMARIKY 1108

  Fly  1436 IYLND-THHYCLHLDGGLVIDGQRMGSDCRFVNHSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGE 1499
            .:.|| ||.|.|.:|...:||....|:..||:||||:||||..||:|||.:|:.|||...|..|.
  Rat  1109 AHENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQPNCETLKWTVNGDTRVGLFAVCDIPAGT 1173

  Fly  1500 ELTYDYNFSLFNPSEGQPCRCNTPQCRGVIGGKSQRVKPLPAVEAKPSGEGLSGRNGRQRKQKAK 1564
            |||::||..... :|...|||....|.|.:|.:       |......|.|        ::.:|||
  Rat  1174 ELTFNYNLDCLG-NEKTVCRCGASNCSGFLGDR-------PKTSTSLSSE--------EKSKKAK 1222

  Fly  1565 KHAQRQAGK 1573
            |..:|:..|
  Rat  1223 KKTRRRRAK 1231

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ash1	NP_524160.2	PHA03247	<1018..1281	CDD:223021	46/307 (15%)
		AWS	1340..1388	CDD:197795	23/49 (47%)
		SET_ASH1L	1391..1531	CDD:380951	59/140 (42%)
		Bromo_ASH1	1680..1787	CDD:99955
		PHD_ASH1L	1858..1900	CDD:277023
		BAH_polybromo	1929..2073	CDD:240068
Nsd2	NP_001178481.2	PWWP_NSD2_rpt1	220..347	CDD:438990	26/141 (18%)
		HMG-box_NSD2	452..507	CDD:438807	6/54 (11%)
		TNG2	<546..710	CDD:227367	43/186 (23%)
		PHD1_NSD1_2	669..711	CDD:277118	12/46 (26%)
		PHD_SF	716..762	CDD:473978	13/76 (17%)
		PHD3_NSD2	763..816	CDD:277124	12/63 (19%)
		PHD_SF	833..873	CDD:473978	10/89 (11%)
		PWWP_NSD2_rpt2	879..974	CDD:438993	18/130 (14%)
		AWS	1012..1062	CDD:197795	23/49 (47%)
		SET_NSD2	1062..1203	CDD:380988	60/142 (42%)
		PHD5_NSD2	1241..1283	CDD:277130
		C5HCH	1282..1327	CDD:465605