Protein Alignment AGO2 and Ago1

DIOPT Version :10

• Sequence 1: NP_730054.1
• Gene: AGO2 / 39683
• FlyBaseID: FBgn0087035
• Length: 1217
• Species: Drosophila melanogaster
• Sequence 2: NP_001304110.1
• Gene: Ago1 / 313594
• RGDID: 1304619
• Length: 895
• Species: Rattus norvegicus
• Alignment Length: 838
• Identity: 278/838 - (33%)
• Similarity: 416/838 - (49%)
• Gaps: 77/838 - (9%)

  Fly   389 PPQQQ--QAAPLPLPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDVKIMPER-PKKF 450
            ||.||  ||     |.:|.    .||:|||.::..||.::|:.|:.  .|||:|.|.|:: |::.
  Rat    15 PPLQQVFQA-----PRRPG----IGTVGKPIKLLANYFEVDIPKID--VYHYEVDIKPDKCPRRV 68

  Fly   451 YRQAFE----QFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVT-DRNGRTLRYTIEIKET 510
            .|:..|    .|:....|.....||||.:.|:|..||:.::..:..|| ...|:...:.:.||..
  Rat    69 NREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWL 133

  Fly   511 GDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIRVGRSFFKMSDPNN-RHELDDGY 574
              :.:..:.|...:.......|:.::|.::|.:......:...||||||  |.|.. .|.|..|.
  Rat   134 --AIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFF--SPPEGYYHPLGGGR 194

  Fly   575 EALVGLYQAFMLGDRP-----FLNVDISHKSFPISMPMIEYLERFSLKAKINNTTNLDYSR---- 630
            |...|.:|:.    ||     .||:|:|..:|..:.|:||::      .::.:..|:|...
  Rat   195 EVWFGFHQSV----RPAMWKMMLNIDVSATAFYKAQPVIEFM------CEVLDIRNIDEQPKPLT 249

  Fly   631 -----RFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFE---HDGKKV--TIASYF 685
                 ||.:. ::|:.|..|.....:   |.|||..::|.|||.:||.   ..|:.|  |:|.||
  Rat   250 DSQRVRFTKE-IKGLKVEVTHCGQMK---RKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYF 310

  Fly   686 HSR-NYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVRKR 749
            ..: |..||:|.|.||.||...|...||:|:|:|..||...:|....|.:.|||..|.|...|:.
  Rat   311 KQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQE 375

  Fly   750 KIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEY--HSKRFTMVKNGSWRMDGMKF 812
            :|..|::...:||||.|..|||::.:|...|:.|||..|.::|  .::.......|.|.|.|.:|
  Rat   376 EISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQF 440

  Fly   813 ---LEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFTDDERSL 874
               :|.|..|..|..    |:  ::.....|.:|.:.:....|...:.:.....:..:.....|:
  Rat   441 YNGIEIKVWAIACFA----PQ--KQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSV 499

  Fly   875 DTIFADLKR--SQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILL 937
            :.:|..||.  |...|.|||:|.....|..:|:..:...|:.|||::...|. |.:.||:.|:.|
  Rat   500 EPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV-KTSPQTLSNLCL 563

  Fly   938 KINSKLNGINHKIKDDPRLPMMKN-TMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYR 1001
            |||.||.|||:.:....|..:.:. .:::|||||||.....:.||:..|..|.|.:.:.|....|
  Rat   564 KINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVR 628

  Fly  1002 LQRGALEEIEDMFSITLEHL-RVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKV 1065
            :||...|.|||:..:..|.| :.||..| ..|..||:|||||.:||.|:|.:.||..|:.||.|:
  Rat   629 VQRPRQEIIEDLSYMVRELLIQFYKSTR-FKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKL 692

  Fly  1066 --GCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGT 1128
              ..:|.|..::|.||||||.|.:.......|..|:..||.||..|.||.|..|::.||..||||
  Rat   693 ERDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGT 757

  Fly  1129 AKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYL 1186
            ::|:.|.|:.:......|.||.|||.|||.:.||.||||.|||||.|.|||.|.|.:|
  Rat   758 SRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHL 815

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO2	NP_730054.1	ArgoN	471..543	CDD:465134	16/72 (22%)
		ArgoL1	554..601	CDD:462567	20/52 (38%)
		PAZ	605..719	CDD:239207	40/128 (31%)
		Piwi_ago-like	764..1186	CDD:240015	155/432 (36%)
Ago1	NP_001304110.1	ArgoN	34..164	CDD:465134	34/133 (26%)
		ArgoL1	174..224	CDD:462567	21/55 (38%)
		PAZ_argonaute_like	225..345	CDD:239212	40/129 (31%)
		Piwi_ago-like	390..815	CDD:240015	155/432 (36%)