Protein Alignment Best4 and BEST4

DIOPT Version :8

• Sequence 1: NP_001097609.1
• Gene: Best4 / 39660
• FlyBaseID: FBgn0036491
• Length: 526
• Species: Drosophila melanogaster
• Sequence 2: NP_695006.1
• Gene: BEST4 / 266675
• HGNCID: 17106
• Length: 473
• Species: Homo sapiens
• Alignment Length: 552
• Identity: 209/553 (38%)
• Similarity: 293/553 (53%)
• Gaps: 127/553 (23%)

  Fly     1 MTVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLVNEEGKRFFEA 65
            |||:||.:|:.:. ...|.|||:|||||||||::.:.::|..||.:|:||||||:.:|.:..:..
Human     1 MTVSYTLKVAEAR-FGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQ 64

  Fly    66 IMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDDRARVMRRTIL 130
            :..||.::..||||||||||:|.:|:||||.|||:||.||.:..:||.|:||.|.|.|::|||::
Human    65 VARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLI 129

  Fly   131 RYVCLCQVIVFTMISPRVKRRFPTYTQIIEAGFLLENEKKIIEAMDQAFPSYPKHWMPIVWAASI 195
            ||..|..|:|...:|.||.:||||...:::|||:.:.|:|..|::...|   .|:|:|.||..::
Human   130 RYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDF---NKYWVPCVWFTNL 191

  Fly   196 VMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQ 260
            ..:|||:.:||||.|:..:::|||:.|..|..|.:|||||:|||||||||:|.||||..|::|:|
Human   192 AAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQ 256

  Fly   261 WNESHS--------------------DEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGED 305
            :.|..:                    |.|     .:.|:.|:||||||.|||||||.:|||||||
Human   257 FVEPEAGAAKPQKLLKPGQEPAPALGDPD-----MYVPLTTLLQFFFYAGWLKVAEQIINPFGED 316

  Fly   306 DDDFELNWIIDRNLTVSYCIVDEMHQEHPELVKDQYWEEVFPNEIPYAQPKMRQNPPAASTAYME 370
            |||||.|.:|||||.||...||||:|..|...|||||:|        .||:    ||.......|
Human   317 DDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDE--------DQPQ----PPYTVATAAE 369

  Fly   371 SQSKLQKRHESAMSTLSTRSRNNFRQNFLGRPSATGSYSDSIQMEDGPSRVTFSENQFDLPHDEA 435
            |.                            |||..|| :.:::|.|.|.:        .|..:.:
Human   370 SL----------------------------RPSFLGS-TFNLRMSDDPEQ--------SLQVEAS 397

  Fly   436 NSSHKPSQSTIDFNDLIEQRRRERRERIRHRFLDMQSTHLRHDTSGAPYTITVL----------R 490
            ..|.:|:.:.              :..:..|||.:          |||.....|          |
Human   398 PGSGRPAPAA--------------QTPLLGRFLGV----------GAPSPAISLRNFGRVRGTPR 438

  Fly   491 PP---------------SDETLSESQEAGDRA 507
            ||               :.....||.|:||.|
Human   439 PPHLLRFRAEEGGDPEAAARIEEESAESGDEA 470

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Best4	NP_001097609.1	Bestrophin	8..319	CDD:279411	156/331 (47%)
BEST4	NP_695006.1	Bestrophin	8..330	CDD:307280	156/331 (47%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930				C63317130
eggNOG	1	0.900				E1_KOG3547
Hieranoid	1	1.000
Homologene	1	1.000				H129743
Inparanoid	1	1.050	396	1.000	Inparanoid score	I1938
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010
OrthoDB	1	1.010				D145964at33208
OrthoFinder	1	1.000				FOG0000272
OrthoInspector	1	1.000				T130037
orthoMCL	1	0.900				OOG5_149342
Panther	1	1.100			O	PTHR10736
Phylome	1	0.910		0.889	Consistency score
RoundUp	1	1.030		avgDist	Average_Evolutionary_Distance	R2974
TreeFam	1	0.960
User_Submission	0	0.000	Not matched by this tool.
	14	13.800